Diagnosis of tospoviruses by reverse-transcription polymerase chain reaction

Acta Horticulturae

Volumn 431, Issue , 1996, Pages 159-166

  Weekes, R J ^a   Mumford, R A ^a   Barker, I ^b   Wood, K R ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b DEFRA   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

IMPATIENS; IMPATIENS NECROTIC SPOT VIRUS; LYCOPERSICON ESCULENTUM; TOMATO SPOTTED WILT VIRUS;

EID: 0004375234     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/ActaHortic.1996.431.12     Document Type: Conference Paper

References (12)

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2. de Haan, P., Kormelink, R., Resende, R. de O., Van Poelwijk, F., Peters, D. and Goldbach, R. 1991. TSWV L RNA encodes a putative RNA polymerase. J. Gen. Virol. 71,2207-2216.
3. de Haan, P., de Avila, A.C., Kormelink, R., Westerbroek, A., Gielen, J.J.L., Peters, D., and Goldbach, R. 1992. The nucleotide sequence of the S RNA of Impatiens necrotic spot virus, a novel tospovirus. FEBS Lett. 306, 27-32.
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8. Maliyakal, E.J. 1992. An efficient method for isolation of RNA and DNA from plants containing polyphenolics. Nucleic Acids Res. 20, 2381.
9. Mukhopadhyay, T. and Roth, J.A. 1993. Silicone lubricant enhances recovery of nucleic acids after phenol-chloroform extraction. Nucleic Acids Res. 21, 781-782.
10. Mumford, R.A. 1995. PhD thesis, University of Birmingham, Birmingham, UK.
11. Mumford, R.A., Barker, I., and Wood, K.R. 1994. The detection of tomato spotted wilt virus using the polymerase chain reaction. J. Virol. Methods, 46, 303-311.
12. Nolasco, G., de Bias, C, Torres, V., and Ponz, F. 1993. A method combining IC and PCR in a microtitre plate for the detection of plant viruses and sub-viral pathogens. J. Virol. Methods, 45, 201-218.