Identification of dry-pinned museum specimens of the sibling species Anopheles gambiae and An.arabiensis (Diptera: Culicidae) using non-radioactively labelled DNA probes

Systematic Entomology

Volumn 22, Issue 2, 1997, Pages 173-180

  Vij, Rajni ^a,b   Harbach, Ralph E ^a   Service, M W ^a   Crampton, Julian M ^a   Molyneux, D H ^a  

^a DEPARTMENT OF LIFE SCIENCES   (United Kingdom)

^b LIVERPOOL SCHOOL OF TROPICAL MEDICINE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANOPHELES GAMBIAE; CUCURBITA; CULICIDAE; DIPTERA;

EID: 0003323458     PISSN: 03076970     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-3113.1997.d01-37.x     Document Type: Article

References (17)

1. Dillon, N., Austin, A.D. & Bartowsky, E. (1996) Comparison of preservation techniques for DNA extraction from hymenopterous insects. Insect Molecular Biology, 5, 21-24.
2. Gillies M.T. & Coetzee, M. (1987) A supplement to the Anophelinae of Africa south of the Sahara (Afrotropical Region). Publications of the South African Institute for Medical Research, 55, 1-143.
3. Harbach, R.E. (1994) Review of the internal classification of the genus Anopheles (Diptera: Culicidae): the foundation for comparative systematics and phylogenetic research. Bulletin of Entomological Research, 84, 331-342.
4. Hill, S.M. & Crampton, J.M. (1994a) Synthetic DNA probes to identify members of the Anopheles gambiae complex and to distinguish the two major vectors of malaria within the complex, Anopheles gambiae s.s. and Anopheles arabiensis. American Journal of Tropical Medicine and Hygiene, 50, 312-321.
5. Hill, S.M. & Crampton, J.M. (1994b) DNA-based methods for the identification of insect vectors. Annals of Tropical Medicine and Parasitology, 88, 227-250.
6. Hill, S.M., Urwin, R. & Crampton, J.M. (1991) A comparison of non- radioactive labeling and detection systems with the synthetic oligonucleotide probes for the species identification of mosquitoes in the Anopheles gambiae complex. American Journal of Tropical Medicine and Hygiene, 44, 609-622.
7. Hill, S.M., Urwin, R. & Crampton, J.M. (1992) A simplified, non- radioactive DNA probe protocol for the field identification of insect vector specimens. Transactions of the Royal Society of Tropical Medicine and Hygiene, 86, 213-215.
8. International Code of Zoological Nomenclature (1985) Third edition. International Trust for Zoological Nomenclature, London, xx + 338 pp.
9. Paskewitz, S.M. & Collins, F.H. (1990) Use of the polymerase chain reaction to identify mosquito species of the Anopheles gambiae complex. Medical and Veterinary Entomology, 4, 367-373.
10. Post, R.J., Flook, P.K. & Millest, A.L. (1993) Methods for the preservation of insects for DNA studies. Biochemical Systematics and Ecology, 21, 85-92.
11. Reiss, R. A., Schwert, D.P. & Ashworth, A.C. (1995) Field preservation of Coleoptera for molecular genetic studies. Environmental Entomology, 24, 716-719.
12. Ribbands, C.R. (1944) Differences between Anopheles melas (A. gambiae var. melas) and Anopheles gambiae. II. Salinity relations of larvae and maxillary palp banding of adult females. Annals of Tropical Medicine and Parasitology, 38, 87-99.
13. Service, M.W. (1982) Importance of vector ecology in vector disease control in Africa. Bulletin of the Society of Vector Ecologists, 7, 1-13.
14. Service, M.W. (1988) New tools for old taxonomic problems in bloodsucking insects. In: Biosystematics of Haematophagous Insects (ed. by M. W. Service), pp. 325-345. The Systematics Association, special volume no. 37, Clarendon Press, Oxford.
15. Vij, R. (1995) DNA probes and cytogenetics for the identification of Anopheles gambiae sibling species. PhD Thesis, University of Liverpool.
16. White, G.B. & Muniss, J.N. (1972) Taxonomic value of spermatheca size for distinguishing four members of the Anopheles gambiae complex in East Africa. Bulletin of the World Health Organization. 46, 793-799.
17. Zahar, A.R. (1993) Review of advances made in the recognition of members of the Anopheles gambiae complex and their bionomics in the Afrotropical Region. In: Entomologist Extraordinary (ed. by M. Coetzee), pp. 64-77. South African Institute for Medical Research, Johannesburg.