Comparative PTA staining of molluscan spermatozoa

Journal of Submicroscopic Cytology and Pathology

Volumn 30, Issue 1, 1998, Pages 183-187

  Sousa, M ^a   Guerra, R ^b   Oliveira, E ^b   Torres, A ^b  

^a UNIVERSITY OF PORTO   (Portugal)

^b NONE

Author keywords

Acrosome; Cytochemistry; Mollusca; PTA staining; Spermatazoa

Indexed keywords

EID: 0003243609     PISSN: 11229497     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. AZEVEDO C., CASTILHO F. and BARANDELA T., 1986. Silver staining analysis on spermatozoa of Nucella lapillus (Gastropoda, Prosobranchia). Int. J. Invert. Reprod. Dev., 9, 299-307.
2. AZEVEDO C. and OLIVEIRA E., 1988. Presence of argyrophilic cytoplasmic and nuclear proteins in the spermatic cells of Nucella lapillus (Gastropoda, Prosobranchia). J. Submicrosc. Cytol. Pathol., 20, 81-87.
3. BOZZO M.G., RIBES E., SAGRISTA E., POQUET M. and DURFORT M., 1993. Fine structure of the spermatozoa of Crassostrea gigas (Mollusca, Bivalvia). Mol. Reprod. Dev., 34, 206-211.
4. ENDO S., 1976. Silver methenamine and phosphotungstic acid staining of the acrosome of Mytilus edulis. Exp. Cell Res., 100, 71-78.
5. FRANZEN A., 1955. Comparative morphological investigations into the spermiogenesis among Mollusca. Zool. Bijdr. Uppsala, 30, 399-456.
6. FRANZEN A., 1970. Phylogenetic aspects of the morphology of spermatozoa and spermiogenesis. In: 'Comparative Spermatology'. Baccetti B. ed., Academic Press, New York, pp. 29-46.
7. GUERRA R., CAMPOS B. and ESPONDA P., 1994. Analysis of the spermatozoa of four bivalves with particular reference to the acrosome and plasma membrane glycoproteins. J. Submicrosc. Cytol. Pathol., 26, 489-495.
8. HEALY J.M., 1995. Comparative spermatozoal ultrastructure and its taxonomic and phylogenetic significance in the bivalve order Veneroida. In: 'Advances in Spermatozoal Phylogeny and Taxonomy'. Jamieson B.G.M., Ausio J. and Justine J.-E. eds., Mém. Mus. Hist. Nat., 166, 155-166.
9. HODGSON A.N., 1995. Spermatozoal morphology of Patellogastropoda and Vetigastropoda (Mollusca: Prosobranchia). In: 'Advances in Spermatozoal Phylogeny and Taxonomy'. Jamieson B.G.M., Ausio J. and Justine J.-L. eds., Mém. Mus. Natl. Hist. Nat., 166, 167-178.
10. JAMIESON B.G.M., 1987. A biological classification of sperm types, with special reference to Annelids and Molluscs, and an example of spermiocladistics. In: 'New Horizons in Sperm Cell Research'. Mohri H. ed., Japan Scientific Press, Tokyo and Gordon & Breach, New York, pp. 311-332.
11. POPHAM J.D., 1979. Comparative spermatozoon morphology and bivalve phylogeny. Malacol. Rev., 12, 1-20.
12. ROUSE G.W. and JAMIESON B.G.M., 1987. An ultrastructural study of the spermatozoa of the polychaetes Eurythoe complanata (Amphinomidae), Clymenella sp. and Micromaldane sp. (Maldanidae), with definition of sperm types in relation to reproductive biology. J. Submicrosc. Cytol. Pathol., 19, 573-584.
13. SOUSA M. and AZEVEDO C., 1988. Comparative silver staining analysis on spermatozoa of various invertebrate species. Int. J. Invert. Reprod. Dev., 13, 1-8.
14. SOUSA M. and OLIVEIRA E., 1994a. Ultrastructural and cytochemical study of spermatogenesis in Donax trunculus (Mollusca, Bivalvia). J. Submicrosc. Cytol. Pathol., 26, 305-311.
15. SOUSA M. and OLIVEIRA E., 1994b. An ultrastructural study of spermatogenesis in Helcion pellucidus (Gastropoda, Prosobranchia). Inv. Reprod. Dev., 26, 119-126.
16. SOUSA M. and OLIVEIRA E., 1994c. An ultrastructural study of Crassostrea angulata (Mollusca, Bivalvia) spermatogenesis. Mar. Biol., 120, 545-551.
17. SOUSA M., OLIVEIRA E., CARVALHEIRO J. and OLIVEIRA E., 1995. Comparative silver staining of molluscan spermatozoa. In: 'Advances in Spermatozoal Phylogeny and Taxonomy'. Jamieson B.G.M., Ausio J. and Justine J.-L. eds., Mém. Mus. Natl. Hist. Nat., 166, 179-187.