Differentiation of yam virus isolates using symptomatology, western blot assay, and monoclonal antibodies

Journal of Phytopathology

Volumn 144, Issue 5, 1996, Pages 235-240

  Goudou Urbino, C ^a,e   Givord, L ^c,e   Quiot, J B ^d,e   Boeglin, M ^d,e   Konate, G ^b,e   Dubern, J ^a,e  

^a INSTITUT DE RECHERCHE POUR LE DÉVELOPPEMENT   (France)

^b Laboratoire de Virologie   (France)

^c INSTITUT DE BIOLOGIE MOLÉCULAIRE ET CELLULAIRE   (France)

^d CEMAGREF   (France)

^e Lab Phytovirologie Regions Chaudes

Author keywords

[No Author keywords available]

Indexed keywords

DIOSCOREA; DIOSCOREA ALATA; YAM MOSAIC VIRUS;

EID: 0003125601     PISSN: 09311785     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1439-0434.1996.tb01522.x     Document Type: Article

References (19)

1. Anonymous (1988): FAO Production Year Book, 41. FAO, Rome.
2. Boeglin, M., G. Labonne, L. Degras, L. Quiot-Douine, J. B. Quiot (1993): Mise en évidence de la présence du virus de la mosaique de l'igname sur igname (Dioscorea trifida) en Guyane. Communication to the Caribbean Food Crop Society Martinique, July 1993.
3. Clark. B. R., E. Engvall (1980): Enzyme linked immunosorbent assay (ELISA): theoretical and practical aspects In E. T. Maggio (ed.) Enzyme Immunosassay, pp. 167-179. CRC Press, Boca Raton, FL.
4. Fazekas de St Groth, S., D. Scheidegger (1980): Production of monoclonal antibodies: strategy and tactics. J. Immunol. Meth. 35, 1-21.
5. Garnsey, S. M., C T. Henderson (1982): Procedures for "bead" ELISA. Workshop on Plant Virus Detection, Puerto Rico, pp. 88-105.
6. Goudou-Urbino, C., G. Konate, J B. Quiot, J. Dubern (1996): Etiology and ecology of a yam mosaic disease in Burkina Faso. Trop. Sci., 36, 34-40.
7. Laemmli, U. K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
8. Lecoq, H., D. E. Purcifull (1992): Biological variability of potyviruses, an example: zucchini yellow mosaic virus. In. Barnet, O. W. (Ed.) Potyvirus Toxonomy, pp. 229-234, Arch. Virol. (Suppl. 5) Springer-Verlag, Wien.
9. Marchoux, G. (1980): Pathologie des ignames en Guadeloupe; maladies virales In: Les Colloques de l'I.N.R.A., l'Igname. Séminaire International de Pointe-à-Pitre, 28 July-02 August 1980, pp. 93-105.
10. Migliori, A., B. Cadilhac (1976): Contribution à l'étude de la maladie à virus de l'igname D. trifida en Guadeloupe. Ann. Phytopathol. 8, 73-78
11. Porth, A., D.-E. Lesemann, H. J. Vetten (1987): Characterization of potyvirus isolates from West African yams (Dioscorea spp). J. Phytophatology 120, 166-183.
12. Quiot-Douine, L., H. Lecoq, J. B. Quiot, M. Pitrat, G Labonne (1990). Serological and biological variability of virus isolates related to strains of papaya ringspot virus. Phytopathology 80, 256-263.
13. Reckhaus, P , F Nienhaus (1981): Etiology of a virus disease of white yam (D. rotundata) in Togo. J. Plant Dis. Prot. 88, 492-509
14. Stocker, J W., H. K. Foster, V. Miggiano, C Stahli, G. Staiger, B. Takacs, and Th Staehelin (1982): Generation of two new myeloma cell lines 'PAF' and 'PAI-O' for hybridoma production. Res. Disclosure, 217, 155-157.
15. Terry. E. R. (1976): Incidence, symptomatology and transmission of a yam virus in Nigeria Proc. Fourth Symp. Inter. Soc. Trop. Root Crops, Cali, Columbia, pp. 170-173.
16. Thouvenel, J.-C. and C. Fauquet (1979): Yam mosaic, a new potyvirus infecting Dioscorea cayenensis in the Ivory Coast. Ann. Appl. Biol. 93, 279-283.
17. Thouvenel, J.-C., C. Fauquet (1980): Utilisation de la technique ELISA dans le diagnostic de la mosaïque de l'igname. Compte rendus de la Deuxième Conférence Internationale sur l'Impact des Maladies à Virus sur le Développement des Pays Africains et du Moyen-Orient. Nairobi, Kenya, 02-06 décembre 1980.
18. Torrance, L (1992): Serological methods to detect plant viruses: Production and use of monoclonal antibodies. In: Duncan, J. M. and L. Torrance (eds), Techniques for the Rapid Detection of Plant Pathogens. Blackwell, Oxford, UK.
19. Towbin, H , Th. Staehelin, J. Gordon (1979): Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl Acad. Sci USA 76, 4350-4354.