β4 integrin transfection of UM-UC-2 (human bladder carcinoma) cells: Stable expression of a spontaneous cytoplasmic truncation mutant with rapid loss of clones expressing intact β4

Cancer Research

Volumn 57, Issue 1, 1997, Pages 38-42

  Kim, Sang Yoon ^a,c   Bachman, Nancy J ^a   Nair, Thankam S ^a   Goldsmith, Scott ^a   Liebert, Monica ^b   Grossman, H Barton ^b   Lomax, Margaret I ^a   Carey, Thomas E ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

^b UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

^c University of Ulsan College of Medicine   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

INTEGRIN;

ARTICLE; BLADDER CARCINOMA; CANCER CELL CULTURE; FLOW CYTOMETRY; GENE EXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOBLOTTING; IMMUNOPRECIPITATION; MOLECULAR CLONING; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; TUMOR GROWTH;

ANTIGENS, CD; BLOTTING, WESTERN; FLOW CYTOMETRY; HUMANS; INTEGRIN ALPHA6; INTEGRIN BETA4; NEOPLASM PROTEINS; TRANSFECTION; TUMOR CELLS, CULTURED; URINARY BLADDER NEOPLASMS;

EID: 0002775846     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Lee, E. C., Lotz, M. M., Steele, G. D. Jr., and Mercurio, A. M. The integrin α6β4 is a laminin receptor. J. Cell Biol., 117: 671-678, 1992.
2. Niessen, C. M., Hogervorst, F., Jaspars, L. H., de Melker, A. A., Delwel, G. O., Hulsman, E. H. M., Kuikman, I., and Sonnenberg, A. The α6β4 integrin is a receptor for both laminin and kalinin. Exp. Cell Res., 211: 360-367, 1994.
3. Hogervorst, F., Kuikman, I., von dem Borne, A. E. G., and Sonnenberg, A. Cloning and sequence analysis of beta-4 cDNA: an integrin subunit that contains a unique 118 kd cytoplasmic domain. EMBO J., 9: 765-770, 1990.
4. Stepp, M. A., Spurr-Michaud, S., Tisadale, A., Elwell, J., and Gipson, K. α6β4 integrin heterodimer is a component of hemidesmosomes. Proc. Nat. Acad. Sci. USA, 87: 8970-8974, 1990.
5. Carter, W. G., Kaur, P., Gil, S. G., and Wayner, E. A. Distinct function for integrins α3β1 in focal adhesions and α6α4/bullous pemphigoid antigen in a new stable anchoring contact (SAC) of keratinocytes: relation to hemidesmosomes. J. Cell Biol., 111: 3141-3154, 1990.
6. Spinardi, L., Ren, Y-L., Sanders, R., and Giancotti, F. G. The β4 subunit cytoplasmic domain mediates the interaction of the α6β4 integrin with the cytoskeleton of hemidesmosomes. Mol. Biol. Cell, 4: 871-884, 1993.
7. Spinardi, L., Einheber, S., Cullen, T., Milner, T., and Giancotti, F. G. A recombinant tail-less integrin β4 subunit disrupts hemidesmosome but does not suppress α6β4-mediated cell adhesion to laminins. J. Cell Biol., 129: 473-487, 1995.
8. Mainiero, F., Pepe, A., Wary, K. K., Spinardi, L., Mohammadi, M., Schlessinger, J., and Giancotti, F. G. Signal transduction by the α6β4 integrin: distinct β4 subunit sites mediate recruitment of Shc/Grb2 and association with the cytoskeleton of hemidesmosomes. EMBO J., 14: 4470-4481, 1995.
9. Clarke, A. S., Lotz, M. M., Chao, C. and Mercurio, A. M. Activation of the p21 pathway of growth arrest and apoptosis by the β4 integrin cytoplasmic domain. J. Biol. Chem., 270: 22673-22676, 1995.
10. Kennel, S. J., Foote, L. J., Falcioni, R., Sonnenberg, A., Stringer, C. D., Grouse, C., and Hemler, M. E. Analysis of the tumor-associated antigen TSP-180: identity with α6β4 in the integrin superfamily. J. Biol. Chem., 264: 15515-15521, 1989.
11. E/β4) from human epithelial cells suggests a fourth family of integrin adhesion receptors. EMBO J., 8: 673-680, 1989.
12. Van Waes, C., Kozarsky, K. F., Warren, A. B., Kidd, L., Paugh, D., Lieben, M., and Carey, T. E. The A9 antigen associated with aggressive human squamous carcinoma has structural and functional similarity to the newly defined integrin α6β4. Cancer Res., 51: 2395-2404, 1991.
13. Liebert, M., Wedemeyer, G., Stein, J. A., Washington, R. W., Jr., Van Waes, C., Carey, T. E., and Grossman, H. B. The monoclonal antibody BQ 16 identifies the α6β4 integrin on bladder cancer. Hybridoma, 12: 67-80, 1993.
14. Kimmel, K. A., and Carey, T. E. Altered expression in squamous cells of an orientation restricted epithelial antigen detected by monoclonal antibody A9. Cancer Res., 46: 3614-3623, 1986.
15. Wolf, G. T,. Carey, T. E., Schmaltz, S. P., McClatchey, K. D., Poore, J., Glaser, L., Hayashida, D. J. S., and Hsu, S. Altered antigen expression predicts outcome in squamous cell carcinoma of the head and neck. J. Natl. Cancer Inst., 82: 1566-1572, 1990.
16. Falcioni, R., Kennel, S. J., Giacomini, P., Zupi, G., and Sacchi, A. Expression of tumor antigen correlated with metastatic potential of Lewis lung carcinoma and B16 melanoma clones in mice. Cancer Res., 46: 5772-5778, 1986.
17. Grossman, H. B., Wedemeyer, G., and Ren, L. UM-UC-1 and UM-UC-2: characterization of two new human transitional cell carcinoma cell lines. J. Urol., 132: 834-837, 1984.
18. Kennel, S. J., Epler, R. G., Lankford, T. K., Foote, L. J., Dickas, V., Canamucio, M., Cavalierie, R., Cosimelli, M., Venture, I., Falcioni, R., and Sacchi, A. Second generation monoclonal antibodies to the human integrin α6β4. Hybridoma, 9: 243-255, 1990.
19. Giancotti, F. G., Stepp, M. A., Suzuki, S., Engvall, E., and Ruoslahti, E. Proteolytic processing of endogenous and recombinant β4 integrin subunit. J. Cell Biol., 118: 951-959, 1992.
20. Jones, J. C. R., Kurpakus, M. A., Cooper, H. M., and Quaranta, V. A function for the integrin α6β4 in the hemidesmosome. Cell Regul., 2: 427-438, 1991.
21. Shaw, L. M., Chao, C., Wewer, U. M., and Mercuric, A. M. Function of the integrin α6β1 in metastatic breast carcinoma cells assessed by expression of a dominant-negative receptor. Cancer Res., 56: 959-963, 1996.