Detection of filarial antigen by inhibition enzyme linked immunosorbent assay using fractionated Brugia malayi microfilarial excretory secretory antigen

Journal of Biosciences

Volumn 21, Issue 1, 1996, Pages 27-34

  Chenthamarakshan, V ^a   Reddy, M V R ^a   Harinath, B C ^a  

^a MAHATMA GANDHI INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Brugia malayi; Diagnosis; ELISA; Excretory secretory antigen; Filariasis; Wuchereria bancrofti

Indexed keywords

ANIMALIA; BRUGIA MALAYI; WUCHERERIA BANCROFTI;

EID: 0002570802     PISSN: 02505991     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02716810     Document Type: Article

References (14)

1. Alikhan A, Parkhe K A, Reddy M V R and Harinath B C 1990 Filarial antigen, antibody and circulating immune complexed antigen levels in Bancroftian filariasis by Stick ELISA; Natl. Med. J. India 3 265-267
2. Avarameas S 1969 Coupling of enzymes to proteins with glutaraldehyde-use of conjugates for the detection of antigen and antibodies; Immunochemistry 6 43-52
3. Chandrashekar R, Rao U R, Rajashekariah G R and Subrahmanyam D 1984 Isolation of microfilariae from blood on iso-osmotic percoll gradients; Indian J. Med. Res. 79 447-450
4. Cheirmaraj K, Parkhe K A and Harinath B C 1992 Evaluation of fractionated circulating filarial antigen in diagnosis of bancroftian filariasis; J. Trop. Med. Hyg. 95 47-51
5. Cheirmaraj K, Reddy MVR and Harinath B C 1990 Diagnostic use of polyclonal antibodies raised in mouse ascitic fluid in bancroftian filariasis; J. Immunoassay 11 429-444
6. Hamilton R G, Hussian R and Ottesen E A 1984 Immunoradiometric assay for detection of filarial antigen in human sera; J. Immunol. 133 2237-2242
7. Lowry O H, Rosenbrough N J, Farr A L and Rundall R J 1951 Protein measurement with the Folin-phenol reagent; J. Biol. Chem. 193 265-275
8. Kharat I, Ghirnikar S N and Harinath B C 1983 immunofluorescence studies with W. bancrofti microfilariae ES antigen; Indian J. Med. Res. 78 497-507
9. Maihotra A and Harinath B C 1984 Detection and monitoring of microfilarial ES antigen levels by inhibition ELISA during DEC therapy; Indian J. Med. Res. 79 194-198
10. Overkamp D. Mohammed Ali S, Carteledge G and Landon J 1988 Production of polyclonal antibodies in ascitic fluid of mice: Techniques and applications; J. Immunoassay 9 51-68
11. Parkhe K A, Reddy M V R, Cheirmaraj K, Ramaprasad P and Harinath B C 1990 Analysis, characterization and diagnostic use of circulating filarial antigen in bancroftian filariasis; J. Biosci 15 37-46
12. Reddy M V R, Malhotra A and Harinath B C 1994a Detection of circulating filarial antigen in bancroftian filariasis by sandwhich ELISA using filarial serum IgG; J. Helminthol. 58 259-262
13. Reddy MVR, Harinath B C and Robert Hamilton R G 1984b Detection of filrial antigen in urine of humans with Wuchereria bancrofti infection by immunoradiometri assay; Indian J. Exp. Biol. 22 515-519
14. Zheng H, Tao Z, Reddy MVR, Harinath B C and Peissens W F 1987 Parasite antigens in sera and urine of patients with bancroftian and brugian filariasis detected by sandwich ELISA with monoclonal antibodies; Am. J. Trop. Med. Hyg. 36 554-560