Identification of the four species of human malaria parasites by nested PCR that targets variant sequences in the small subunit rRNA gene

Parasitology International

Volumn 46, Issue 2, 1997, Pages 91-95

  Kimura, Masatsugu ^a   Kaneko, Osamu ^a   Liu, Qing ^b   Zhou, Mian ^b   Fumihiko, Kawamoto ^b   Wataya, Yusuke ^c   Otani, Shuzo ^a   Yamaguchi, Yoshiko ^a   Tanabe, Kazuyuki ^d  

^a OSAKA CITY UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b NAGOYA UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^c OKAYAMA UNIVERSITY   (Japan)

^d OSAKA INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

Human malaria parasites; Identification of species; Nested PCR; PCR; Plasmodium; rRNA; Small subunit rRNA

Indexed keywords

EID: 0002560231     PISSN: 13835769     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5769(97)00013-5     Document Type: Article

References (13)

1. Snounou, G., Viriyakosol, S., Jarra, W., Thaithong, S. and Brown, K.N. (1993) Identification of the four human malaria parasite species in field samples by the polymerase chain reaction and detection of a high prevalence of mixed infections. Mol. Biochem. Parasitol. 58, 283-292.
2. Arai, M., Kunisada, K., Kawai, S., Kimura, M. and Wataya, Y. (1994) DNA diagnosis of ovale malaria and malariae malaria using microtiter plate-hybridization. Nucleosides Nucleotides 13, 1363-1374.
3. Snounou, G., Viriyakosol, S., Zhu, X.P., Jarra, W., Pinheiro, L., do Rosario, V.E., Thaithong, S. and Brown, K.N. (1993) High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol. Biochem. Parasitol. 61, 315-320.
4. Li, J., Wirtz, R.A., McConkey, G.A., Sattabongkot, J., Waters, A.P., Rogers, M.J. and McCutchan, T.F. (1995) Plasmodium: genus-conserved primers for species identification and quantitation. Exp. Parasitol. 81, 182-190.
5. Kawamoto, F., Miyake, H., Kaneko, O., Kimura, M., Dung, N. Thi, Dung, N. The, Liu, Q., Zhou, M., Dao, L. D., Kawai, S., Ismoura, S. and Wataya, Y. (1996) Sequence variation in the 18S rRNA gene, a target for PCR-based malaria diagnosis, in Plasmodium ovale for Southern Vietnam. J. Clinic. Microbiol. 34, 2287-2288.
6. Kimura, M., Kaneko, O., Inoue, A., Ishii, A. and Tanabe, K. (1995) Amplification by polymerase chain reaction of Plasmodium falciparum DNA from Giemsastained thin blood smears. Mol. Biochem. Parasitol. 70, 193-197.
7. Wooden, J., Kyes, S. and Sibley, C.H. (1993) PCR and strain identification in Plasmodium falciparum. Parasitol. Today 9, 303-305.
8. Li, J., McConkey, G.A., Rogers, M.J., Waters, A.P. and McCutchan, T.R. (1994) Plasmodium: the developmentally regulated ribosome. Exp. Parasitol. 78, 437-441.
9. Kawamoto, F. (1991) Rapid diagnosis of malaria by fluorescence microscopy with light microscope and interference filter. Lancet 337, 200-202.
10. In two cases of the four, band forms specific for P. malariae were seen in acridine orange-stained thin smears. Comparison of the morphologies of the same parasites stained with acridine orange and then Giemsa will be published elsewhere.
11. McCutchan, T.F., de la Cruz, V.F., Lal, A.A., Gunderson, J.H., Elwood, H.J. and Sogin, M.L. (1988) Primary sequences of two small subunit ribosomal RNA genes from Plasmodium falciparum. Mol. Biochem. Parasitol. 28, 63-68.
12. Goman, M., Mons, B. and Scaife, J. (1991) The complete sequence of a Plasmodium malariae SSUrRNA gene and its comparison to other plasmodial SSUrRNA genes. Mol. Biochem. Parasitol. 45, 281-288.
13. Qari, S.H., Goldman, I.F., Pieniazek, N.J., Collins, W.E. and Lal, A.A. (1994) Blood and sporozoite stage-specific small subunit ribosomal RNA-encoding genes of the human malaria parasite Plasmodium vivax. Gene 150, 43-49.