Effects of surfactant retreatment in vitro: A method to evaluate changes in cell junctions and in cell viability

Alternatives to Laboratory Animals

Volumn 24, Issue 5, 1996, Pages 859-865

  Clothier, Richard ^a   Sansom, Rebekah ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Alamar Blue; Fluorescein leakage; In vitro; Repeat treatment

Indexed keywords

EID: 0002531416     PISSN: 02611929     EISSN: None     Source Type: Journal    
DOI: 10.1177/026119299602400512     Document Type: Article

References (10)

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2. Cottin, M., Dossou, K.G., De Silva, O., Tolle, M., Roguet, R., Cohen, C., Catroux, P., Delabarre, L, Sicard, C. & Rougier, A. (1995). Relevance and reliability of in vitro methods in ocular safety assessment. In Alternative Methods in Toxicology, Vol. 11, The World Congress on Alternatives and Animal Use in the Life Sciences: Education, Research, Testing (ed. A.M. Goldberg & L.F.M. Van Zutphen), pp. 323-331. New York: Mary Ann Liebert.
3. Garle, M.J , Fentem, J.H. & Fry, J.R. (1994). In vitro eytotoxicity tests for the prediction of acute toxicity in vivo Toxicology in Vitro 8, 1303-1312.
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5. Tchao, R (1987). Trans-epithelial permeability of fluorescein in vitro as an assay to determine eye irritants. In Alternative Methods in Toxicology, Vol 6, Progress in In Vitro Toxicology (ed. A.M. Goldberg), pp. 271-283. New York: Mary Ann Liebert
6. Balls, M., Botham, P.A., Bruner, L.H. & Spielmann, H (1995). The EC/HO international validation study on alternatives to the Draize eye irritation test Toxicology in Vitro 9, 871-929.
7. Page, B., Page, M. & Noel, C. (1993). A new fluorometric assay for cytotoxicity measurements in vitro. International Journal for Oncology 3, 473-476.
8. Goegan, P., Johnson, G. & Vincent, R. (1995). Effects of serum protein and colloid on the Alamar Blue ™ assay in cell cultures. Toxicology in Vitro 9, 257-266.
9. Clothier, R.H., Morgan, S.J., Atkinson, K A., Garle, M.J. & Balls, M. (1994). Development of a fixed-dose approach for the fluorescein leakage test. Toxicology in Vitro 8, 883-884.
10. Ward, R., Shaw, B., Clothier, R. & Balls, M. (1993). Development of a human epidermal equivalent in vitro. Journal of Anatomy 182, 151.