Monoclonal Antibody Production in Gas-Permeable Flexible Flasks, Using Serum-Free Media

Contemporary Topics in Laboratory Animal Science

Volumn 37, Issue 6, 1998, Pages 55-60

  Stang, Bernadette V ^a   Wood, Patricia A ^b   Reddington, John J ^c   Reddington, Ginger M ^c   Heidel, Jerry R ^a  

^a OREGON STATE UNIVERSITY   (United States)

^b Biomedical Research Institute   (United States)

^c DiagXotics Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0002505737     PISSN: 10600558     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Goding, J. W. 1980. Antibody production by hybridomas. J. Immunol. Methods. 39:285-308.
2. Cleveland, W. L., I. Wood, and B. F. Erlanger. 1983. Routine large-scale production of monoclonal antibodies in a protein-free culture medium. J. Immunol. Methods. 56:221-234.
3. Sjögren-Jansson, E. and S. Jeansson. 1985. Large-scale production of monoclonal antibodies in dialysis tubing. J. Immunol. Methods. 84:359-364.
4. Stocks, S. J. and D. E. Brooks. 1989. Production and isolation of large quantities of monoclonal antibody using serum-free medium and fast protein liquid chromatography. Hybridoma. 2:241-247.
5. Backer, M. P., L. S. Metzger, P. L. Slaber, et al. 1988. Large-scale production of monoclonal antibodies in suspension culture. Biotech. Bioeng. 32:993-1000.
6. Klerx, J. P. A. M., C. J. Verplanke, C. G. Blonk, et al 1988. In vitro production of monoclonal antibodies under serum-free conditions using a compact and inexpensive hollow fibre cell culture unit. J. Immunol. Methods. 111:179.
7. Pannell, R. and C. Milstein. 1992. An oscillating bubble chamber for laboratory scale production of monoclonal antibodies as an alternative to ascitic tumours. J. Immunol. Methods. 146:43-48.
8. Sjögren-Jansson, E., M. Ohlin, C. A. K. Borrebaeck, et al. 1991. Production of human monoclonal antibodies in dialysis tubing. Hybridoma. 10:411-419.
9. Barnes, D. and G. Sato. 1980. Methods for growth of cultured cells in serum-free media. Anal. Biochem. 102:255-270.
10. Barnes, D. and G. Sato, G. 1980. Serum-free cell culture: a unifying approach. Cell. 22:649-655.
11. Graf, H., J-N. Rabaud, and J-M. Egly. 1991. Screening of various mammalian cell culture media to establish a downstream purification scheme. J. Immunol. Methods. 139:135-144.
12. Barnes, D. 1987. Serum-free animal cell culture. BioTechniques. 5:534-541.
13. Kolanko, W. 1986. A flexible plastic container suitable for the scale-up and routine cultivation of mammalian cells in suspension. World Biotech Report 1986, Vol. 2:49-66. November, 1986. Online Publications, NY.
14. Kolanko, W. and R. Symbol. 1987. Improved yields of monoclonal antibodies in flexible plastic containers. World Biotech Report 1987, Vol. 3:151-162. November, 1987. Online Publications, NY.
15. Yannelli, J. R., G. B. Thurman, S. G. Dickerson, et al. 1987. An improved method of the generation of human lymphokine activated killer cells. J. Immunol. Methods. 100:137-145.
16. Sun, C-S. and J. Chapman. 1991. Propagation of human immunodeficiency virus in flexible tissue culture vessels. BioP. April, Vol. 4:34-36.
17. Heidner, H. W., N. J. MacLachlan, F. J. Fuller, et al. 1988. Bluetongue virus genome remains stable throughout prolonged infection of cattle. J. Gen. Virol. 69:2629-2636.
18. Rockey, D. D., C. F. Dungan, T. Lunder, et al 1991. Monoclonal antibodies against Aeromonas salmonicida lipopolysaccharide identify differences among strains. Dis. Aquatic Organ. 10:115-120.
19. Wiens, G. D. and S. L. Kaattari. 1989. Monoclonal antibody analysis of common surface protein(s) of Renibacterium salmoninarum. Fish Pathol. 24:1-7.
20. Caswell-Reno, P., V. Lipipun, P. W. Reno, et al. 1989. Use of a group-reactive and other monoclonal antibodies in an enzyme immunodot assay for identification and presumptive serotyping of aquatic birnaviruses. J. Clin. Micro. 27:1924.
21. Mullins, T. D., J. L. Dzimian, R. M. Fike, et al. 1991. Superior hybridoma growth and monoclonal antibody production in a new serum-free medium: Hybridoma-SFM. Focus 13:91-93.
22. Patterson, M. K., Jr. 1979. Measurement of growth and viability of cells in culture. Methods Enzymol. 58:141-152.
23. Fike, R., J. Pfohl, D. Jayme, et al. 1990. Protein-free hybridoma media. Focus 12:79-80.
24. Tarleton, R. L. and A. M. Beyer, A.M. 1991. Medium-scale production and purification of monoclonal antibodies in protein-free medium. BioTechniques. 11:590-593.
25. Wolfe, R. A., J. A. Braatz, D. A. Miller, et al 1988. A new serum-free medium for monoclonal antibody production. BioTechniques 6:62-67.
26. Arkoosh, M. R. and S. L. Kaattari. 1990. Quantitation of fish antibody to a specific antigen by an enzyme-linked immunosorbent assay (ELISA), p. 15-24. In Stolen, J. S., T. C. Fletcher, D. P. Anderson, B. S. Roberson, and W. B. Van Muiswinkel (eds.) Techniques in fish immunology. SOS Publications, Fair Haven, N.J.
27. Reddington, J. J., G. M. Reddington, and N. J. MacLachlan. 1991. A competitive ELISA for detection of antibodies to the group antigen of bluetongue virus. J. Vet. Diagn. Invest. 3:144-147.
28. Ozturk, S. S. and B. Ø. Palsson. 1990. Loss of antibody productively during long-term cultivation of a hybridoma cell line in low serum and serum-free media. Hybridoma. 9:167-175.
29. Eagle, H. and K. Piez. 1962. The population-dependent requirement by cultured mammalian cells for metabolites which they can synthesize. J. Exp. Med. 116:29-43.