Raising of antibodies against Aeromonas salmonicida ssp. salmonicida in chicken and isolation of the immunoglobulins (IGY) from the egg yolk

Bulletin of the European Association of Fish Pathologists

Volumn 16, Issue 2, 1996, Pages 47-50

  Denzin, N ^a   Henrion, E ^a  

^a Fed Inst Hlth Protect C   (Germany)

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EID: 0002457468     PISSN: 01080288     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (13)

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2. Austin, B. and Austin, D. A. (1987). Bacterial fish pathogens: disease in farmed and wild fish. John Wiley and sons, Chichester.
3. Bernoth, E.-M. (1990a). Screening for the fish disease agent Aeromonas salmonicida by an enzyme-linked immunosorbent assay (ELISA). J. Aquatic Animal Health, 2, 99-103.
4. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Blochem., 72, 248-254.
5. Gottstein, B. and Hemmeler, E. (1985). Egg yolk: immunoglobulin Y as an alternative antibody in the serology of echinococcosis. Z Parasitenkd, 71, 273-276.
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8. Leslie, G. A. and Clem, L. W. (1969). Phylogeny of immunoglobulin structure and function. III. Immunoglobulins of the chicken. J. Exp. Med., 130, 1337-1352.
9. Locken, M. R. and Roth, T. F. (1983). Analysis of maternal IgG subpopulations which are transported in the chicken oocyte. Immunol., 49, 21-28.
10. Patterson, R., Youngner, J. S., Weigle, W. O. and Dixon, F. J. (1962). The metabolism of serum proteins in the hen and chick and secretion of serum proteins by the ovary of the hen. J. Gen. Physiol., 45, 501-503.
11. Polsen, A., von Wechmar, M. B. and van Regenmortel, M. H. V. (1980). Isolation of viral IgY antibodies from yolks of immunised hens. Immunol. Comm., 9, 475-493.
12. Rose, M. E., Orlans, E., and Buttress, N. (1974). Immunoglobulin classes in the hen's egg: their segregation in yolk and white. Eur. J. Immunol., 4, 521-523.
13. Wallmann, J., Staak, C. and Luge, E. (1990). Einfache Methode zur Isolierung von Immunglobulinen (Y) aus Eiern immunisierter Hühner. J. Vet. Med. B, 37, 317-320.