Use of confocal laser scanning microscope in conjunction with a conduction heat transfer stage in order to observe dynamically the freeze-thaw cycle in an autofluorescent substance and to measure ice crystal size in situ

Cryobiology

Volumn 33, Issue 1, 1996, Pages 27-33

  Evans, J ^a   Adler, J ^a   Mitchell, J ^a   Blanshard, J ^a   Rodger, G ^b  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^b Marlow Foods Ltd   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

FUSARIUM; GIBBERELLA ZEAE;

EID: 0002127406     PISSN: 00112240     EISSN: None     Source Type: Journal    
DOI: 10.1006/cryo.1996.0003     Document Type: Article

References (8)

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2. Donhowe, D. P., Hartel, R. W., and Bradley, R. L. Determination of ice crystal size distributions in frozen desserts. J. Dairy Sci. 74, 3334-3344, (1991).
3. Mackenzie, A. P., Kuster, T. A., and Luyet, B. J. Freeze-fixation at high subzero temperatures. Cryobiology 12, 427-439, (1975).
4. Masters, B. R. Confocal microscopy and three-dimensional reconstruction of thick, transparent, vital tissue. Scan. Microsc. Suppl. 6, 71-79, (1992).
5. Muhr, A. H., and Blanshard, J.M. V. Effect of polysaccharide stabilizers on the rate of growth of ice. J. Food Technol. 21, 683-710, (1986).
6. Muhr, A. H., Blanshard, J.M. V., and Sheard, S. H. Effect of polysaccharide stabilizers on the nucleation of ice. J. Food Technol. 21, 587-603, (1986).
7. Ploem, J. S. Laser scanning fluorescence microscopy. Appl. Optics 26, 3226-3231, (1987).
8. Shotten, D. M. Confocal scanning optical microscopy and its application for biological specimens. J. Cell Sci. 94, 175-206, (1989).