Characterization of the cardiac holotroponin complex reconstituted from native cardiac troponin T and recombinant I and C

European Journal of Biochemistry

Volumn 261, Issue 1, 1999, Pages 40-47

  Reiffert, Silke ^d   Maytum, Robin ^a,c   Geeves, Michael ^a,c   Lohmann, Karin ^d   Greis, Thomas ^d   Blüggel, Martin ^d   Meyer, Helmut E ^d   Heilmeyer, Ludwig M G ^d   Jaquet, Kornelia ^b  

^a MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^b RUHR UNIVERSITY BOCHUM   (Germany)

^c UNIVERSITY OF KENT   (United Kingdom)

^d NONE

Author keywords

Calcium regulation; Cardiac troponin; Cardiac troponin I mutants; Phosphorylation; Reconstitution

Indexed keywords

CYCLIC AMP DEPENDENT PROTEIN KINASE; MUTANT PROTEIN; MYOSIN SUBFRAGMENT 1; RECOMBINANT PROTEIN; TROPONIN; TROPONIN C; TROPONIN I; TROPONIN T;

AMINO ACID SUBSTITUTION; ARTICLE; CALCIUM BINDING; CONFORMATIONAL TRANSITION; ESCHERICHIA COLI; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; THIN FILAMENT;

ANIMALS; BASE SEQUENCE; BINDING SITES; CALCIUM; CATTLE; DNA PRIMERS; ESCHERICHIA COLI; HUMANS; MACROMOLECULAR SUBSTANCES; MUTAGENESIS, SITE-DIRECTED; MYOCARDIUM; PHOSPHORYLATION; RABBITS; RECOMBINANT PROTEINS; TROPONIN; TROPONIN C; TROPONIN I; TROPONIN T;

EID: 0002035194     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00261.x     Document Type: Article

References (31)

1. 1. Mittmann, K., Jaquet, K. & Heilmeyer, L.M.G. Jr (1990) A common motif of two adjacent phosphoserines in bovine, rabbit and human cardiac troponin I. FEBS Lett. 273, 41-45.
2. 2. Mittmann, K., Jaquet, K. & Heilmeyer, Jr L.M.G. (1992) Ordered phosphorylation of a duplicated minimal motif for the cAMP dependent protein kinase present in cardiac troponin I. FEBS Lett. 302, 133-137.
3. 3. Jaquet, K., Thieleczek, R. & Heilmeyer, L.M.G. Jr (1995) Pattern formation on cardiac troponin I by consecutive phosphorylation and dephosphorylation. Eur. J. Biochem. 231, 486-490.
4. 4. Quirk, P.G., Patchell, V.B., Gao, Y., Levine, B.A. & Perry, S.V. (1995) Sequential phosphorylation of adjacent serine residues on the N-terminal region of cardiac troponin I. Structure-activity implications of ordered phosphorylation. FEBS Lett. 370, 175-178.
5. 5. Ardelt, P., Jaquet, K., Dorka, P., Heilmeyer, L.M.G., Körtke, H., Körfer, R. & Notohamiprodjo, G. (1998) Microanalysis and distribution of cardiac troponin I phospho species in heart areas. Biol. Chem. 379, 341-347.
6. 6. Reiffert, S.U., Jaquet, K., Heilmeyer, Jr L.M.G. & Herberg, F.W. (1998) Stepwise subunit interaction changes by mono-and bisphosphorylation of cardiac troponin I. Biochemistry 39, 13516-13525.
7. 7. Zhang, R., Zhao, J. & Potter, J.D. (1995) Phosphorylation of both serine residues in cardiac troponin I is required to decrease the Ca (2+) affinity to cardiac troponin C. J. Biol. Chem. 270, 30773-30780.
8. 8. Reiffert, S.U., Jaquet, K., Heilmeyer, Jr L.M.G., Ritchie, M.D. & Geeves, M.A. (1996) Bisphosphorylation of cardiac troponin I modulates the Ca (2+)-dependent binding of myosin subfragment S1 to reconstituted thin filaments. FEBS Lett. 384, 43-47.
9. 9. Zhang, R., Zhao, J., Mandveno, A. & Potter, J.D. (1995) Cardiac troponin I phosphorylation increases the rate of cardiac muscle relaxation. Circ. Res. 76, 1028-1035.
10. 10. Johns, E.C., Simnett, S.J., Mulligan, I.P. & Ashley, C.C. (1997) Troponin I does not increase the rate of relaxation following laser flash photolysis of diazo-2 in guinea-pig skinned trabeculae. Pfluegers Arch. Eur. J. Physiol. 433, 842-844.
11. 11. Smillie, L.B. (1985) Preparation and identification of a and α tropomyosin. Methods Enzymol. 85, 234-241.
12. 12. Spudich, J.A. & Watt, S. (1971) The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of tropmyosin-troponin complex with actin and the proteolytic fragments of myosin. J. Biol. Chem. 246, 4866-4871.
13. 13. Criddle, A.H., Geeves, M.A. & Jeffries, T. (1985) The use of actin labelled with (N-(I-pyrenyl) iodoacetamide to study the interaction of actin with myosin subfragments and troponin/tropomyosin. Biochem. J. 232, 343-349.
14. 14. Weeds, A.G. & Taylor, R.S. (1975) Separation of subfragment-1 isoenzymes from rabbit skeletal muscle myosin. Nature 257, 54-56.
15. 15. Beier, N., Jaquet, K., Schnackerz, K. & Heilmeyer, Jr L.M.G. (1988) Isolation and characterisation of a highly phosphorylated cardiac troponin from bovine heart. Eur. J. Biochem. 176, 327-334.
16. 16. Villar-Palasi, C. & Kumon, A. (1981) Purification and properties of dog cardiac troponin T kinase. J. Biol. Chem. 256, 7409-7415.
17. 17. Stull, J.T. & Buss, J.E. (1977) Phosphorylation of cardiac troponin by cyclic adenosine 3: 5′ monophosphate dependent protein kinase. J. Biol. Chem. 252, 851-857.
18. 18. Greaser, M.L. & Gergerly, J. (1971) Reconstitution of troponin activity from three protein components. J. Biol. Chem. 246, 4226-4233.
19. 19. Cole, H.A. & Perry, S.V. (1975) The phosphorylation of troponin I from cardiac muscle. Biochem. J. 149, 525-533.
20. 20. Babu, A., Su, H., Ryu, Y. & Gulati, J. (1992) Determination of residue specificity in the EF hand of troponin C for Ca2+ coordination by genetic engineering. J. Biol. Chem. 267, 15469-15474.
21. 21. AI-Hillawi, E., Minchin, S.D. & Trayer, I.P. (1994) Overexpression of human cardiac troponin I and troponin C in Escherichia coli and their purification and characterisation. Two point mutations allow high-level expression of troponin-I. Eur. J. Biochem. 225, 1195-11201.
22. 22. Herberg, F.W., Bell, S.M. & Taylor, S.S. (1993) Expression of the catalytic subunit of cAMP dependent protein kinase in Escherichia coli: multiple isoenzymes reflect different states. Protein Eng. 6, 771-777.
23. 23. O'Farrell, P.Z., Goodman, H.M. & O'Farrell, P.H. (1977) High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12, 1133-1142.
24. 24. Swiderek, K., Jaquet, K., Meyer, H.E. & Heilmeyer, Jr L.M.G. (1988) Cardiac troponin I, isolated from bovine heart, contains two adjacent phosphoserines. A first example of phosphoserine determination by derivatization to S-ethylcysteine. Eur. J. Biochem. 176, 335-342.
25. 25. Head, J.G., Ritchie, M.D. & Geeves, M.A. (1995) Characterisation of the equilibrium between blocked and closed states of muscle thin filaments. Eur. J. Biochem. 227, 694-699.
26. 26. McKillop, D.F. & Geeves, M.A. (1991) Regulation of the acto.myosin subfragment 1 interaction by troponin/tropomyosin. Evidence for control of a specific isomerization between two acto.myosin subfragment 1 states. Biochem. J. 279, 711-718.
27. 27. Noland, T.A. Jr, Guo, X., Raynor, R.L., Jidema, N.M., Averyhart-Fullard, V., Solaro, R.J. & Kuo, J.F. (1995) Cardiac troponin I mutants. Phosphorylation by proteinkinases C and A and regulation of Ca (2+) stimulated MgATPase of reconstituted actomyosin S1. J. Biol. Chem. 270, 25445-25454.
28. 31P-NMR spectroscopy. Biochemistry 32, 13873-13878.
29. 29. Jaquet, K., Lohmann, K., Czisch, M., Holak, T., Gulati, J. & Jaquet, R. (1998) A model for the function of the bisphosphorylated heart-specific troponin I N-terminus. J. Muscle Res. Cell Motil. 19, 647-659.
30. 30. Fujita-Becker, S., Kluwe, L., Miegel, A., Maeda, K. & Maeda, Y. (1993) Reconstitution of rabbit skeletal muscle troponin from recombinant subunits all expressed in and purified in E. coli. J. Biochem. 114, 438-444.
31. 31. Malhotra, A., Nakouzi, A., Bowman, J. & Buttrick, P. (1997) Expression and regulation of mutant forms of cardiac TnI in a reconstituted actomyosin system: role of kinase dependent phosphorylation. Mol. Cell. Biochem. 170, 99-107.