Catalytic antibodies: Regio- and enantioselectivity in a hetero Diels-Alder reaction

Pure and Applied Chemistry

Volumn 68, Issue 11, 1996, Pages 2025-2028

  Resmini, Marina ^a   Meekel, Arthur A P ^a   Pandit, Upendra K ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0001764387     PISSN: 00334545     EISSN: None     Source Type: Journal    
DOI: 10.1351/pac199668112025     Document Type: Article

References (20)

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14. 1 (K light chain).
15. The background reaction was run simultaneously with each antibody-catalyzed reaction, in order to ensure constant experimental conditions.
16. 2O 4:6 containing 0.1% TFA, 1.5 ml/min, with UV detection at 282 nm, retention time for substrate 2 8.9 min, for product 4 28 min and for product 3 31.5 min. Data are uncorrected for the background reaction, which under these conditions is concurrently taking place.
17. 2, filtered and evaporated. Samples were analyzed using cyclodextrin-modified micellar electrokinetic chromatography. Conditions: capillary 90 cm (60 cm to the detector), 50 μm internal diameter; separation solution: 50 mM sodium deoxycholate in 6 mM borate buffer pH 9 containing 35 mM α-cyclodextrin, applied voltage 25 kV; UV detection at 282 nm; retention times for the enantiomers of 4 28.7 min and 29.6 min, for 3 31.1 min. Under these conditions only the separation of the two enantiomers of 4 was achieved.
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