Antibodies can recognize the chiral center of free α-amino acids

Journal of the American Chemical Society

Volumn 120, Issue 13, 1998, Pages 3251-3252

  Hofstetter, Oliver ^a,b,c   Hofstetter, Heike ^a,b,c   Schurig, Volker ^a,b,c   Wilchek, Meir ^a,b,c   Green, Bernard S ^a,b,c  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^c HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0001759906     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja973680n     Document Type: Letter

References (26)

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15. The end-point titers of total sera obtained were 1:>500000 for antiD-AA and 1:>250000 for anti-L-AA, respectively. There was no detectable recognition of BSA alone. For further use, antibodies were enriched by precipitation with ammonium sulfate and dialyzed against phosphate-buffered saline to give final concentrations of 19.3 mg/mL (anti-D-AA) and 21.2 mg/ mL (anti-L-AA).
16. 50) was determined by interpolation assuming linearity near the point of 50% inhibition.
17. (b) Competitive ELISA procedures provide relative affinities rather than real affinities. For a critical discussion on the determination of affinities by ELISA, see: Goldberg, M. E.; Djavadi-Ohaniance, L. Curr. Opin. Immunol. 1993, 5, 278-281.
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19. We found group-selective specificity for the α-amino acid stereogenic center using a total of 31 amino acids with a different, more sensitive assay (unpublished results).
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21. Preliminary results also suggest that these antibodies may be used for quantification of enantiomeric purity, especially at the outer limits of current methods for estimating enantiomer ratios. Antibody anti-D-AA has been successfully used for this purpose, and the results will be reported separately.
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