Purification and characterization of a cryoprotective protein (cryoprotectin) from the leaves of cold-acclimated cabbage

Plant Physiology

Volumn 111, Issue 1, 1996, Pages 215-221

  Sieg, Frank ^a   Schröder, Werner ^a   Schmitt, Jürgen M ^a   Hincha, Dirk K ^a  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0001737252     PISSN: 00320889     EISSN: None     Source Type: Journal    
DOI: 10.1104/pp.111.1.215     Document Type: Article

References (27)

1. Arnon DJ (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24: 1-15
2. Blum H, Beier H, Gross HJ (1987) Improved silverstaining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8: 93-99
3. Calvete JJ, Sous D, Sanz L, Diaz-Maurino T, Töpfer-Petersen E (1994) Glycosylated boar spermadhesin AWN-1 isoforms. Biological origin, structural characterization by lectin mapping, localization of O-glycosylation sites, and effect of glycosylation on ligand binding. Biol Chem Hoppe-Seyler 375: 667-673
4. Debray H, Decout D, Strecker G, Spik G, Montreuil J (1981) Specificity of twelve lectins towards oligosaccharides and glycopeptides related to N-glycosylproteins. Eur J Biochem 177: 41-55
5. Dwek RA, Edge CJ, Harvey DJ, Wormald MR (1993) Analysis of glycoprotein-associated oligosaccharides. Annu Rev Biochem 62: 65-100
6. Görg A, Postel W, Westermaier R (1978) Ultrathin-layer isoelectric focusing in polyacrylamide gels on cellophane. Anal Biochem 89: 60-70
7. Guy CL (1990) Cold acclimation and freezing stress tolerance: role of protein metabolism. Annu Rev Plant Physiol Plant Mol Biol 41: 187-223
8. Heber U, Kempfle M (1970) Proteine als Schutzstoffe gegenüber dem Gefriertod der Zelle. Z Naturforsch 25b: 834-842
9. Hincha DK, Heber U, Schmitt JM (1989) Freezing ruptures thylakoid membranes in leaves, and rupture can be prevented in vitro by cryoprotective proteins. Plant Physiol Biochem 27: 795-801
10. Hincha DK, Heber U, Schmitt JM (1990) Proteins from frosthardy leaves protect thylakoids against mechanical freeze-thaw damage in vitro. Planta 180: 416-419
11. Hincha DK, Schmitt JM (1992a) Cryoprotective leaf proteins: assay methods and heat stability. J Plant Physiol 140: 236-240
12. Hincha DK, Schmitt JM (1992b) Freeze-thaw injury and cryoprotection of thylakoid membranes. In GN Somero, CB Osmond, CL Bolis, eds, Water and Life. Springer, Berlin, pp 316-337
13. Hincha DK, Schmitt JM (1995) Long-term cryopreservation of thylakoid membranes by sugars. In JG Day, MR McLellan, eds, Methods in Molecular Biology, Vol 38: Cryopreservation and Freeze-Drying Protocols. Humana Press, Totowa, NJ, pp 71-80
14. Hincha DK, Sieg F, Bakaltcheva I, Köth H, Schmitt JM (1996) Freeze-thaw damage to thylakoid membranes: specific protection by sugars and proteins. In PL Steponkus, ed, Advances in Low-Temperature Biology, Vol 3. JAI Press, London (in press)
15. Johnson DA, Gautsch JW, Sportsman JR, Elder JH (1984) Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose. Gene Anal Tech 1: 3-8
16. Kohnke-Godt B, Gabius H-J (1991) Heparin-binding lectin from human placenta: further characterization of ligand binding and structural properties and its relationship to histones and heparin-binding growth factors. Biochemistry 30: 55-65
17. Krause GH, Grafflage S, Rumich-Bayer s, Somersalo S (1988) Effects of freezing on plant mesophyll cells. Symp Soc Exp Biol 42: 311-327
18. Levitt J (1980) Responses of Plants to Environmental Stresses, Vol 1. Academic Press, Orlando, FL
19. Lynch DV, Steponkus PL (1987) Plasma membrane lipid alterations associated with cold acclimation of winter rye seedlings (Secale cereale L. cv Puma). Plant Physiol 83: 761-767
20. Menozzi FD, Mutombo R, Renauld G, Gantiez C, Hannah JH, Leininger E, Brennan MJ, Locht C (1994) Heparin-inhibitable lectin activity of the filamentous hemagglutinin adhesin of Bordetella pertussis. Infect Immun 62: 769-778
21. Redinbaugh MG, Wilbur HC (1985) Adaptation of the dye-binding protein assay to microtiter plates. Anal Biochem 147: 144-147
22. Schagger H, von Jagow G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166: 368-379
23. Steponkus PL (1984) Role of the plasma membrane in freezing injury and cold acclimation. Annu Rev Plant Physiol 35: 543-584
24. Steponkus PL, Uemura M, Webb MS (1993) A contrast of the cryostability of the plasma membrane of winter rye and spring oat: two species that widely differ in their freezing tolerance and plasma membrane lipid composition. In PL Steponkus, ed, Advances in Low-Temperature Biology, Vol 2. JAI Press, London, pp 211-312
25. Thomashow MF (1993) Genes induced during cold acclimation in higher plants. In PL Steponkus, ed, Advances in Low-Temperature Biology, Vol 2. JAI Press, London, pp 183-210
26. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354
27. Volger HG, Heber U (1975) Cryoprotective leaf proteins. Biochim Biophys Acta 412: 335-349