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Spectrophotometer test tubes (Milton Roy No. 33-17-80, optical path = 1 cm) flattened at the bottom and with glass tubes (8 cm long, 1 cm diameter) fused on their tops were used for measurements of optical density because optical cells were cracked by the gels after several cycles. The optical tubes were closed by attaching to them, via #9 Chem threads, capillaries with a #7 Ace electrode adapter/Teflon septum/nylon bushing combination.
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- ions was possible with gels prepared in quartz cells, since the borosilicate optical tubes cut-off light below 300 nm.
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12
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5244362254
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The analyzing light beam of the spectrophotometer is 1.1 mm wide, and the center of the beam strikes the sample about 9 mm from the bottom of the tubes. To ensure detection of the Au particles during the early stages of their formation, the tubes were raised by 5 mm from their normal position in the sample holder, since the beam extends only 3 mm above and below its center. This caused the lower section of the beam to enter the samples about 0.5 mm above the bottom of the gels.
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