Partial Purification and Characterization of Pectin Methylesterase from Green Beans (Phaseolus vulgaris L.)

Journal of Agricultural and Food Chemistry

Volumn 45, Issue 3, 1997, Pages 572-577

  Laats, Marjet M ^a   Grosdenis, Françoise ^a   Recourt, Kees ^a   Voragen, Alfons G J ^a   Wichers, Harry J ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Isoforms; Pectin methylesterase; Phaseolus vulgaris; Purification; Texture

Indexed keywords

PHASEOLUS VULGARIS;

EID: 0001565981     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf960341p     Document Type: Article

References (31)

1. Andersson, P. A. Modelling of potato blanching. Effects of heat treatment on solute transport and texture. Ph.D. dissertation, University of Lund, Lund, Sweden, 1994.
2. Bertheau, Y.; Madgidi-Hervan, E.; Kotoujanski, A.; Nguyen The, C.; Coleno, A. Detection of depolymerase isoenzymes after electrophoresis or electrofocussing, or in titration curves. Anal. Biochem. 1984, 139, 383-389.
3. Bordenave, M.; Goldberg, R. Purification and characterization of pectin methylesterases from mung bean hypocotyl cell walls. Phytochemistry 1993, 33, 999-1003.
4. Castaldo, D.; Quagliuolo, L.; Servillo, L.; Balestrieri, C.; Giovane, A. Isolation and characterization of pectin methylesterase from apple fruit. J. Food Sci. 1989, 54, 653-655, 673.
5. Fayyaz, A.; Asbi, B. A.; Ghazali, H. M.; Che Man, Y. B.; Jinap, S. Purification and molecular properties of papaya pectinesterase. Food Chem. 1994, 49, 373-378.
6. Gaffe, J.; Morvan, C.; Jauneau, A.; Demarty, M. Partial purification of flax cell wall pectin methylesterase. Phytochemistry 1992, 31, 761-765.
7. Gaffe, J.; Tieman, D. M.; Handa, A. K. Pectin methylesterase isoforms in tomato (Lycopersicon esculentum) tissues. Plant Physiol. 1994, 105, 199-203.
8. Giovane, A.; Quagliuolo, L.; Castaldo, D.; Servillo, L.; Balestrieri, C. Pectin methyl esterase from Actenidia chinensis fruits. Phytochemistry 1990, 29, 2821-2823.
9. Glover, H.; Brady, C. Purification of three pectin esterases from ripe peach fruit. Phytochemistry 1994, 37, 949-955.
10. Goldberg, R.; Pierron, M.; Durand, L.; Mutaftschiev, S. In vitro and in situ properties of cell wall pectin methylesterases from mung bean hypocotyls. J. Exp. Botany 1992, 43, 41-46.
11. Hagerman, A. E.; Austin, P. J. Continuous spectrophotometric assay for plant methylesters. J. Agric. Food Chem. 1986, 34, 440-444.
12. Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
13. Laratta, B.; Loiudice, R.; Giovane, A.; Quagliuolo, L.; Servillo, L.; Castaldo, D. Thermostability of three pectinesterase isoenzymes in tomato fruit. Food Chem. 1995, 52, 415-418.
14. Morrissey, S. H. Silver staining for proteins in polyacrylamide gels, a modified procedure with enhanced uniform sensitivity. Anal. Biochem. 1981, 117, 307-310.
15. Moustacas, A.-M.; Nari, J.; Diamantidis, G.; Noat, G.; Grasnier, M.; Borel, M.; Ricard, J. Electrostatic effects and the dynamics of enzyme reactions at the surface of plant cells. Eur. J. Biochem. 1986, 155, 191-197.
16. Nari, J.; Noat, G.; Ricard, J. Pectin methylesterase, metal ions and plant cell-wall extension. Hydrolysis of pectin by plant cell-wall pectin methylesterase. Biochem. J. 1991, 279, 343-350.
17. Pressey, R.; Woods, F. M. Purification and properties of two pectinesterases from tomatoes. Phytochemistry 1992, 31, 1139-1142.
18. Rillo, L.; Castaldo, D.; Giovane, A.; Servillo, L.; Balestrieri, C.; Quagliuolo, L. Purification and properties of pectin methylesterase from mandarin orange fruit. J. Agric. Food Chem. 1994, 40, 591-593.
19. Seymour, T. A.; Preston, J. F.; Wicker, L.; Lindsay, J. A.; Wei, C.; Marshall, M. R. Stability of pectinesterases of Marsh white grapefruit pulp. J. Agric. Food Chem. 1991a, 39, 1075-1079.
20. Seymour, T. A.; Preston, J. F.; Wicker, L.; Lindsay, J. A.; Marshall, M. R. Purification and properties of pectinesterases of Marsh white grape fruit pulp. J. Agric. Food Chem. 1991b, 39, 1080-1085.
21. Stanley, D. W.; Bourne, M. C.; Stone, A. P.; Wismer, W. V. Low-temperature blanching effects on chemistry, firmness and structure of canned green beans and carrots. J. Food Sci. 1995, 60, 327-333.
22. Steinbuch, E. De stevigheid van diepvries sperziebonen en boterbonen (The firmness of frozen green beans and butter beans). Voedingsmiddelen-technologie (Food Technol.) 1986 (Juni), 19-22.
23. Summers, W. L. Pectinesterase and D-galacturonase activities in eight snap bean cultivars. Hortscience 1989, 24, 484-486.
24. Tieman, D. M.; Handa, A. K. Reduction in methyl esterase activity modifies tissue integrity and cation levels in ripening tomato (Lycopersicon esculentum Mill.) fruits. Plant Physiol. 1994, 106, 429-436.
25. Van Buren, J. P.; Moyer, J. C.; Robinson, W. B. Pectin methylesterase in snap beans. J. Food Sci. 1962, 27, 291-294.
26. Versteeg, C.; Rombouts, F. M.; Pilnik, W. Purification and some characteristics of two pectinesterase isoenzymes from orange. Lebensm.-Wiss. Technol. 1978, 11, 267-274.
27. Versteeg, C.; Rombouts, F. M.; Spaansen, C. H.; Pilnik, W. Thermostability and orange juice cloud stabilizing properties of multiple pectinesterases from orange. J. Food Sci. 1980, 45, 969-971, 998.
28. Warrilow, A.; Jones, M. G. Different forms of tomato pectinesterase have different kinetic properties. Phytochemistry 1995, 39, 277-282.
29. Warrilow, A. G. S; Turner, R. J.; Jones, M. G. A novel form of pectinesterase in tomato. Phytochemistry 1994, 35, 863-868.
30. Wicker, L.; Vassallo, M. R.; Escheverria, E. J. Solubilization of cell wall bound, thermostable pectinesterase from Valencia orange. J. Food Sci. 1988, 53, 1171-1174, 1180.
31. Zhang, J. Pectinesterase and cell wall degradation in normal and transgenic tomatoes. Ph.D. dissertation, University of Nottingham, Leicestershire, U.K., 1994, 217 pp.