Preparation of poly(β-malic acid) by enzymatic ring-opening polymerization of benzyl β-malolactonate

Chemistry Letters

Volumn , Issue 9, 1996, Pages 795-796

  Matsumura, Shuichi ^a   Beppu, Hideki ^a   Nakamura, Kazuhiro ^a   Osanai, Shuichi ^a   Toshima, Kazunobu ^a  

^a KEIO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0001517140     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.1996.795     Document Type: Article

References (12)

1. F. Kawai, Adv. Biochem. Eng./Biotech., 52, 151 (1995).
2. G. Swift, Polym. Degrad. Stabil., 45, 215 (1994).
3. T. Ouchi and A. Fujino, Makromol. Chem., 190, 1523 (1989).
4. Y. Abe, S. Matsumura, and K. Imai, J. Jpn. Oil Chem. Soc., 35, 937 (1986).
5. M. Vert and R.W. Lenz, Polym. Prepr. (Am. Chem. Soc., Div. Polym. Chem.), 20, 608 (1979).
6. S.C. Arnold and R.W. Lenz, Makromol. Chem., Macromol. Symp., 6, 285 (1986).
7. H. Uyama and S. Kobayashi, Chem. Lett., 1993, 1149.
8. R.T. MacDonald, S.K. Pulapura, Y.Y. Svirkin, R.J. Gross, D.L. Kaplan, J. Akkara, G. Swift, and S. Wolk, Macromolecules, 28, 73 (1995).
9. Enzymes: Porcine pancreatic lipase (PP, 41 U/mg protein, according to the supplier) was purchased from Sigma Chemical Co. (St. Louis, MO, USA). Novozyme 435 (triacylglycerol hydrolase + carboxylesterase) having 7000 PLU/g (propyl laurate units) was kindly supplied by Novo Nordisk A/S (Bagsvaerd, Denmark).
10. Benzyl β-D,L-malolactonate (BM) was prepared by the cyclization of benzyl hydrogen bromosuccinate which was prepared using the bromosuccinic anhydride obtained by the treatment of maleic anhydride with hydrogen bromide according to Refs. 4 and 5.
11. The number-averge molecular weight (M̄n), weight-averge molecular weight (M̄w) and molecular weight dispersion (M̄w/M̄n) were measured by a gel permeation chromatography (GPC) using GPC columns (Shodex 80M, Showa Denko Co., Ltd., Tokyo) with a reflective index detector. Chloroform was used as the eluent. The GPC system was calibrated with a polystyrene standard.
12. P. Guerin, M. Vert, C. Braud, and R.W. Lenz, Polym. Bull., 14, 187 (1985).