Mechanism of 3-methylaspartase probed using deuterium and solvent isotope effects and active-site directed reagents: Identification of an essential cysteine residue

Bioorganic and Medicinal Chemistry

Volumn 7, Issue 5, 1999, Pages 949-975

  Pollard, John R ^b   Richardson, Susan ^b   Akhtar, Mahmoud ^b   Lasry, Philippe ^b   Neal, Tracy ^b   Botting, Nigel P ^b   Gani, David ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b NONE

Author keywords

Enzyme inhibitors; Enzymes and enzymic reactions; Isotope effects; Kinetics

Indexed keywords

AMMONIA LYASE; ASPARTIC ACID; ASPARTIC ACID DERIVATIVE; CYSTEINE; DEUTERIUM; ISOTOPE; REAGENT; SOLVENT;

AMINATION; ARTICLE; CHEMICAL MODIFICATION; DEAMINATION; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX;

AMMONIA; AMMONIA-LYASES; BINDING SITES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYSTEINE; DEUTERIUM; DOSE-RESPONSE RELATIONSHIP, DRUG; HYDROGEN-ION CONCENTRATION; KINETICS; MAGNESIUM; MODELS, CHEMICAL; MODELS, MOLECULAR; PROTEIN CONFORMATION; TIME FACTORS;

EID: 0001508740     PISSN: 09680896     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0968-0896(99)00044-9     Document Type: Article

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