Synthesis of a Three-Helix Bundle Protein by Reductive Amination

Journal of Organic Chemistry

Volumn 63, Issue 3, 1998, Pages 728-731

  Tahmassebi, Deborah C ^a   Sasaki, Tomikazu ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0001494674     PISSN: 00223263     EISSN: None     Source Type: Journal    
DOI: 10.1021/jo970812m     Document Type: Article

References (21)

1. (a) Lieberman, M.; Sasaki, T. J. Am. Chem. Soc. 1991, 113, 1470-1471.
2. (b) Ghadiri, M. R.; Soares, C.; Choi, C. J. Am. Chem. Soc. 1992, 114, 825-831.
3. (c) Ghadiri, M. R.; Case, M. A. Angew. Chem., Int. Ed. Engl. 1993, 32, 1594-1597.
4. (a) Sasaki, T.; Kaiser, E. T. J. Am. Chem. Soc. 1989, 777, 380-381.
5. (b) Sasaki, T.; Kaiser, E. T. Biopolymers 1990, 29, 79-88.
6. (c) Akerfeldt, K. S.; Kim, R. M.; Camac, D.; Groves, J. T.; Lear, J. D.; DeGrado, W. F. J. Am. Chem. Soc. 1992, 114, 9656-9657.
7. (a) Tuchscherer, G. Tetrahedron Lett. 1993, 34, 8419-8422.
8. (b) Rose, K. J. Am. Chem. Soc. 1994, 116, 30-33.
9. (c) Dawson, P. E.; Kent, S. B. H. J. Am. Chem. Soc. 1993, 115, 7263-7266.
10. Tahmassebi, D. C.; Sasaki, T. J. Org. Chem. 1994, 59, 679-681.
11. -1), indicating that there is negligible contribution to the CD spectrum by the template itself.
12. (a) Kim, P. S . Baldwin R. L. Annu. Rev. Biochem. 1982, 51, 459-89
13. (b) Ptitsy O. B.; Pain, R.; Semisotnov, G. V.; Zerovnik, E.; Razgulyaev, O. I. Febs. Lett. 1990, 262, 20-4
14. (c) Kawajima, K.; Proteins 1989, 6, 87-103.
15. (d) Lecomte, J. T.; Matthews, C. R. Protein Eng. 1993, 6, 1-10.
16. (a) Stryer, L. J. Mol. Biol. 1965, 13, 482-495.
17. (b) Semisotnov, G. V.; Rodionova, N. A.; Razgulyaev, O. I.; Uversky V. N.; Gripas, A. F.; Gilmanshin, R. I. Biopolymers 1991, 31, 119-128.
18. 3 did not bind ANS to the TRIPOD core. The studies also indicate that the addition of pep has very little effect on the emission of ANS and does not shift the maximum at all.
19. Handel, T.; Williams, S.; DeGrado, W. Science 1993, 261, 879-885.
20. Rink, H. Tetrahedron Lett. 1987, 28, 3787-3790.
21. There is degradation if the product is not frozen directly off of the HPLC. There is also degradation during Sephadex G-15 gel column purification. The product can be isolated with very high purity if frozen directly off of the HPLC.