Enhanced biosensor performance using an avidin-biotin bridge for antibody immobilization

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 2980, Issue , 1997, Pages 187-194

  Narang, Upvan ^a   Anderson, George P ^a   King, Keeley D ^a   Liss, Heidi S ^a   Ligler, Frances S ^a  

^a NAVAL RESEARCH LABORATORY   (United States)

Author keywords

Antibody immobilization techniques; Avidin biotin; Biosensors; Fiber optics

Indexed keywords

ADSORPTION; BACTERIOLOGY; BIOSENSORS; COENZYMES; FIBER OPTICS; FIBERS; FLUORESCENCE; OPTICAL FIBERS; SIGNAL TRANSDUCTION;

ANALYTE; ANTIBODY IMMOBILIZATION TECHNIQUES; AVIDIN-BIOTIN; BACILLUS GLOBIGII; CONFORMATIONAL CHANGES; CONVENTIONAL METHODS; COVALENT ATTACHMENTS; CROSS LINKERS; EVANESCENT WAVES; IMPROVED PERFORMANCES; LINEAR DYNAMIC RANGES; LOWER LIMIT OF DETECTIONS; OVALBUMIN; PARTIAL DENATURATIONS; SPECIFIC DETECTIONS;

ANTIBODIES;

EID: 0001486485     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.273534     Document Type: Conference Paper

References (12)

1. O. S. Wolfbeis. Fiber Optic Chemical Sensors and Biosensors, Vol. I, CRC Press, Boca Raton, 1991.
2. R. A. Ogert, L. C. Shriver-Lake, and F. S. Ligler. "Toxin detection using a fiber optic-based biosensor," SPIE, Vol. 1885, pp. 11-17, 1993.
3. L. A. Templelman, K. D. King, G. P. Anderson, and F. S. Ligler. "Quantitating staphylococcal enterotoxin B in diverse media using a portable fiber optic biosensor," Anal. Biochem., Vol. 223, pp. 50-57, 1996.
4. R. A. Ogert, J. E. Brown, B. R. Singh, L. C. Shriver-Lake, and F. S. Ligler. "Detection of Clostridium botulinum toxin A using a fiber optic-based biosensor," Anal. Biochem., Vol. 205, pp. 306-312, 1992.
5. L. K. Cao, G. P. Anderson, F. S. Ligler, and J. Ezzell. "Detection of Yersinia pestis F1 antigen by a fiber optic biosensor," J. Clin. Micro, Vol. 33, pp. 336-341, 1995.
6. L. C. Shriver-Lake, K. A. Breslin, P. T. Charles, D. W. Conrad, J. P. Golden, and F. S. Ligler. "Detection of TNT in water using an evanescent wave fiber optic biosensor," Anal. Chem., Vol. 67, pp. 2431-2435, 1995.
7. R. F. Taylor. Protein immobilization: Fundamentals and applications, Chap. 8, pp. 263-303, Marcel Dekker, Inc., New York, 1991.
8. H. H. Weetall. Immobilized enzymes, antigens, antibodies, and peptides: Preparation and characterization, Chap. 6, pp. 246-292 and chap. 8, pp. 419-497, Marcell Dekker, New York, 1975.
9. D. Savage, G. Mattson, S. Desai, G. Nielander, S. Morgensen, and E. Conklin. Avidin-biotin chemistry: A handbook. Pierce, Rockford, Illinois, 1994.
10. G. P. Anderson, J. P. Golden, L. K. Cao, D. Wijesuriya, L. C. Shriver-Lake, and F. S. Ligler. "Development of an evanescent wave fiber optic biosensor," IEEE Eng. Med. Biol., Vol. 13, pp. 358-363, 1994.
11. S. K. Bhatia, L. C. Shriver-Lake, K. J. Prior, J. H. Georger, J. M. Calvert, R. Bredehorst, and F. S. Ligler. "Use of thiol-terminal silanes and heterobifunctional crosslinkers for immobilization of antibodies on silica surfaces," Anal. Biochem., Vol. 178, pp. 408-413, 1989.
12. S. Zhao and W. M. Reichert. "Influence of biotin lipid surface density and accessibility on avidin binding to the tip of an optical fiber sensor," Langmuir, Vol. 8, pp. 2785-2791, 1992.