Effect of surfactants on activity and stability of native and chemically modified lipases A and B from Candida rugosa

Biocatalysis and Biotransformation

Volumn 13, Issue 4, 1996, Pages 271-285

  Calvo, María V ^a   Plou, Francisco J ^a   Ballesteros, Antonio ^a  

^a CSIC   (Spain)

Author keywords

Candida rugosa; Chemical modification; Enzyme acylation; Lipases; Polyethylene glycol; Stability

Indexed keywords

EID: 0001432554     PISSN: 10242422     EISSN: None     Source Type: Journal    
DOI: 10.3109/10242429609003605     Document Type: Article

References (25)

1. Alba, F., Arenas, J. C. and Lopez, M. A. (1995) "Properties of rat brain dipeptidyl aminopeptidases in the presence of detergents". Peptides, 16, 325-329.
2. Bardsley, G. W. (1993) SIMFIT: "A package for simulation, curve-fitting and statistical analysis in the life sciences", Manchester University, UK.
3. Bornemann, S., Crout, D. H. G., Dalton, H. and Hutchinson, D. W. (1994) "The effects of surfactants on lipase-catalysed hydrolysis of esters: activities and stereoselectivity". Biocatalysis, 11, 191-211.
4. Calvo, M. V., Plou, F. J., Pastor, E. and Ballesteros, A. (1995) "Effect of chemical modification of isoenzymes A and B from C. rugosa on their activity and stability". Botechnol. Lett., 17, 171-176.
5. Deleuze, H., Langrand, G., Millet, H., Baratti, J., Buono, G. and Triantaphylides, C. (1987) "Lipase-catalyzed reactions in organic media: competition and applications". Biochim. Biophys. Acta, 911, 117-120.
6. Gaertner, H. F. and Puigserver A. J. (1992) "Increased activity and stability of poly(ethylene glycol)-modified trypsin". Enzyme Microb. Technol., 14, 150-155.
7. Habbeb, A. F. S. A. (1966) "Determination of free amino groups in proteins by trinitrobenzenesulfonic acid". Anal. Biochem., 14, 328-336.
8. Ibel, K., May, R. P., Kirschner, K., Szadkowski, H., Mascher, E. and Lundahl, P. (1990) "Protein-decorated micelle structure of sodium-dodecyl-sulfate-protein complexes as determined by neutron scattering". Eur. J. Biochem., 190, 311-318.
9. Jones, M. N. (1992) "Surfactant interactions with biomembranes and proteins". Chem. Soc. Rev., 127-136.
10. Kawasaki, Y., Takahashi, Y., Nakatani, M., Murakami, M., Dosako, S. and Okai, H. (1994) "Enzymatic functions of fatty acid-modified chymotrypsin and trypsin in aqueous organic media". Biosci. Biotech. Biochem., 58, 512-516.
11. Ljunger, G., Adlercreutz, P. and Mattiasson, B. (1993). "Reactions catalyzed by PEG-modified α-chymotrypsin in organic solvents. Influence of water content and degree of modification". Biocatalysis, 7, 279-288.
12. Lotti, M., Tramontano, A., Longhi, S., Fusetti, F., Brocca, S., Pizzi, E. and Alberghina, L. (1994) "Variability within the Candida rugosa lipases family". Protein Eng., 7, 531-537.
13. Malmos, H. (1990) "Enzymes for detergents". Chem. Ind., 183-186.
14. Nascimento, A. E. and Campos-Takaki, G. M. (1994) "Effect of sodium dodecyl sulfate on lipase of Candida lipolytica". Appl. Biochem. Biotechnol., 49, 93-99.
15. Otero, C., Pastor, E., Fernández, V. M. and Ballesteros, A. (1990a) "Influence of the support on the reaction course of tributyrin hydrolysis catalyzed by soluble and immobilized lipases". Appl. Biochem. Biotechnol., 23, 237-247.
16. Otero, C., Pastor, E. and Ballesteros, A. (1990b) "Synthesis of monobutyrylglycerol by transesterification with soluble and immobilized lipases". Appl. Biochem. Biotechnol., 26, 35-44.
17. Pasta, P., Riva, S. and Carrea, G. (1988) "Circular dichroism and fluorescence of polyethylene glycol-subtilisin in organic solvents". FEBS Lett., 236, 329-332.
18. Peled, N. and Krenz, M. C. (1981) "A new assay of microbial lipases with emulsified trioleyl glycerol". Anal. Biochem., 112, 219-222.
19. Plou, F. J. and Ballesteros, A. (1994) "Acylation of subtilisin with long fatty acyl residues affects its activity and thermostability in aqueous medium". FEBS Lett., 339, 200-204.
20. Redondo, O., Herrero, A., Bello, J. F., Roig, M. G., Calvo, M. V., Plou, F. J. and Burguillo, F. J. (1995) "Comparative kinetic study of lipases A and B from Candida rugosa in the hydrolysis of lipid ρ-nitrophenyl esters in mixed micelles with Triton X-100". Biochim. Biophys. Acta, 1243, 15-24.
21. Rúa, M. L., Díaz-Mauriño, T., Fernández, V. M., Otero, C. and Ballesteros, A. (1993) "Purification and characterization of two distinct lipases from Candida cylindracea". Biochim. Biophys. Acta, 1156, 181-189.
22. Rúa, M. L. and Ballesteros, A. (1994) "Rapid purification of two lipase isoenzymes from Candida rugosa". Biotechnol. Tech., 8, 21-26.
23. Shami, E. Y., Rothstein, A. and Ramjeesingh, M. (1989) "Stabilization of biologically active proteins". Trends Biotechnol., 7, 186-190.
24. Stocks, S. J., Jones, A. J. M., Ramey, Ch. W. and Brooks, D. E. (1986) "A fluorimetric assay of the degree of modification of protein primary amines with polyethylene glycol". Anal. Biochem., 154, 232-234.
25. Takahashi, K., Kodera, Y., Yoshimoto, T., Ajima, A., Matsushima, A. and Inada, Y. (1985) "Ester-exchange catalyzed by lipase modified with polyethylene glycol". Biochem. Biophys. Res. Commun., 131, 532-536.