Stress responses in alfalfa (Medicago sativa L.): II. Purification, characterization, and induction of phenylalanine ammonia-lyase isoforms from elicitor-treated cell suspension cultures

Plant Physiology

Volumn 92, Issue 2, 1990, Pages 447-455

  Jorrin, Jesus ^a,b   Dixon, Richard A ^a  

^a PLANT BIOLOGY DIVISION   (United States)

^b UNIVERSITY OF CÓRDOBA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0001360385     PISSN: 00320889     EISSN: None     Source Type: Journal    
DOI: 10.1104/pp.92.2.447     Document Type: Article

References (27)

1. Bolwell GP, Bell JN, Cramer CL, Schuch W, Lamb CJ, Dixon RA (1985) L-Phenylalanine ammonia-lyase from Phaseolus vulgaris. Characterization and differential induction of multiple forms from elicitor-treated cell suspension cultures. Eur J Biochem 149:411-419
2. Bolwell GP, Sap J, Cramer CL, Schuch W, Lamb CJ, Dixon RA (1985) L-Phenylalanine ammonia-lyase from Phaseolus vulgaris: partial degradation of enzyme subunits in vitro and in vivo. Biochim Biophys Acta 881: 210-221
3. Chen R-Y, Chang T-C, Liu M-S (1988) Phenylalanine ammonialyase of bamboo shoots. Agric Biol Chem 52: 2137-2142
4. Cramer CL, Edwards K, Dron M, Liang X, Dildine SL, Bolwell GP, Dixon RA, Lamb CJ, Schuch W (1989) Phenylalanine ammonia-lyase gene organization and structure. Plant Mol Biol 12: 367-383
5. Dalkin K, Edwards R, Edington B, Dixon RA (1989) Stress responses in alfalfa (Medicago saliva L.). I. Elicitor induction of phenylpropanoid biosynthesis and hydrolytic enzymes in cell suspension cultures. Plant Physiol 92: 440-446
6. Dixon RA (1985) Isolation and maintenance of callus and cell suspension cultures. In RA Dixon, ed, Plant Cell Culture. A Practical Approach. IRL Press, Oxford, pp 1-20
7. Dixon RA (1986) The phytoalexin response: elicitation, signalling and control of host gene expression. Biol Rev 61: 239-291
8. Dixon RA, Dey PM, Lamb CJ (1983) Phytoalexins: enzymology and molecular biology. Adv Enzymol 53: 1-136
9. Dixon RA, Lamb CJ (1979) Stimulation of de novo synthesis of L-phenylalanine ammonia-lyase in relation to phytoalexin accumulation in Colletotrichum lindemuthianum elicitor-treated cell suspension cultures of French Bean (Phaseolus vulgaris). Biochim Biophys Acta 586: 453-463
10. Edwards K, Cramer CL, Bolwell GP, Dixon RA, Schuch W, Lamb CJ (1985) Rapid transient induction of phenylalanine ammonia-lyase mRNA in elicitor-treated bean cells. Proc Natl Acad Sci USA 82: 6731-6735
11. Given NK, Venis MA, Grierson D (1988) Purification and properties of phenylalanine ammonia-Iyase from strawberry fruit and its synthesis during ripening. J Plant Physiol 133: 31-37
12. Hames BD (1981) Gel Electrophoresis of Proteins. A Practical Approach. IRL Press, Oxford
13. Jones DH (1986) Phenylalanine ammonia-lyase: regulation of its induction and its role in plant development. Phytochemistry 23: 1349-1359
14. Jorrin J, Lopez-Valbuena R, Tena M (1988) Purification and properties of phenylalanine ammonia-lyase from sunflower (Helianthus annuus L.) hypocotyls. Biochim Biophys Acta 964: 73-82
15. Koster JA, Zuzok A, Barz W (1983) High performance liquid chromatography of isoflavones and phytoalexins from Cicer arietinum. J Chromatogr 270: 392-395
16. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
17. Latunde-Dada AO, Dixon RA, Lucas JA (1987) Induction of phytoalexin biosynthetic enzymes in resistant and susceptible lucerne callus lines infected with Verticillium albo-atrum. Physiol Mol Plant Pathol 31: 15-23
18. Lawton MA, Dixon RA, Hahlbrock K, Lamb CJ (1983) Elicitor induction of mRNA activity. Rapid effects of elicitor on phenylalanine ammonia-lyase and chalcone synthase mRNA activities in bean cells. Eur J Biochem 130: 131-139
19. Lawton MA, Lamb CJ (1987) Transcriptional activation of plant defense genes by fungal elicitor, wounding and infection. Mol Cell Biol 7: 335-341
20. Liang X, Dron M, Cramer CL, Dixon RA, Lamb CJ (1989) Differential regulation of phenylalanine ammonia-lyase genes during plant development and by environmental cues. J Biol Chem 264: 14486-14492
21. Mathur RS, Barnet HL, Lilly VG (1949) Sporulation of Colletotrichum lindemuthianum in culture. Phytopathology 40: 104-111
22. Neuhoff V, Azold N, Taube D, Ehrhardt W (1988) Improved staining of proteins in polyacrylamide gels, including isoelectric focusing gels, with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9: 255-262
23. O'Farrell PH (1975) High resolution two-dimensional electro-phoresis of proteins. J Biol Chem 250: 4007-4021
24. Palmiter RD (1974) Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undegraded polysomes and messenger ribonucleic acid. Biochemistry 13: 3606-3615
25. Read SH, Northcote DH (1981) Minimization of variation in the response to different proteins of the Coomassie blue G dye binding assay for protein. Anal Biochem 116: 53-64
26. Schroder J, Betz B, Hahlbrock K (1976) Light induced enzyme synthesis in cell suspension culture of Petroselinum hortense. Demonstration in a heterologous cell-free system of rapid changes in the rate of phenylalanine ammonia-lyase synthesis. Eur J Biochem 67: 527-541
27. Shahin EA, Spielmann A, Sukhapinda JK, Simpson RB, Yashar M (1986) Transformation of cultivated alfalfa using disarmed Agrobacterium tumefaciens. Crop Sci 26: 1235-1239