Purification and Characterization of Four Pectinesterases from Sweet Cherry (Prunus avium L.)

Journal of Agricultural and Food Chemistry

Volumn 44, Issue 11, 1996, Pages 3416-3422

  Alonso, Jesús ^a   Rodríguez, M Teresa ^a,b   Canet, Wenceslao ^a  

^a INSTITUTO DEL FRÍO CSIC   (Spain)

^b UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

Author keywords

Cell wall; Isoenzymes; Pectinesterase; Prunus avium L; Rosaceae; Sweet cherries

Indexed keywords

MALUS X DOMESTICA; PRUNUS AVIUM; ROSACEAE;

EID: 0001286614     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf960204s     Document Type: Article

References (50)

1. Alonso, J. Estudio de la influencia de distintos tratamientos en la textura de cerezas (Prunus avium L.) congeladas. Función de la pectinesterasa. Ph.D. Dissertation, Universidad Complutense de Madrid, Madrid, Spain, 1993.
2. Alonso, J.; Canet, W.; Rodríguez, M. T. Effect of various thermal pre-treatment on the texture of frozen cherries (Prunus avium L.). Related enzyme activities. Z. Lebensm.-Unters. Forsch. 1993, 196, 214-218.
3. Alonso, J.; Rodríguez, M. T.; Canet, W. Effect of calcium pretreatments in the texture of frozen cherries. role of pectinesterase in the changes in the pectic materials. J. Agric. Food Chem. 1995a, 43, 1011-1016.
4. Alonso, J.; Rodríguez, M. T.; Canet, W. Detection of pectinesterase in polyacrylamide gels. Electrophoresis 1995b, 16, 39-42.
5. Alonso, J.; Howell, N.; Canet, W. Purification and character-ization of persimmon fruit pectinesterases. 7th Cell Wall Meeting; Universidad de Santiago de Compostela: Santiago de Compostela, Spain, 1995c.
6. Balaban, M. O.; Arreola, A. G.; Marshall, M.; Peplow, A.; Wei, C. I.; Cornell, J. Inactivation of pectinesterase in orange juice by supercritical carbon dioxide. J. Food Sci. 1991, 56, 743-746, 750.
7. Baldwin, E. A.; Pressey, R. Tomato polygalacturonase elicits ethylene. Production in tomato fruit. J. Am. Soc. Hortic. Sci. 1988, 113, 92-95.
8. Barrett, D. M.; Gonzalez, C. Activity of softening enzymes during cherry maturation. J. Food Sci. 1994, 59, 574-577.
9. Bartolome, L. G.; Hoff, J. E. Firming of potatoes. Biochemical effects of preheating. J. Agric. Food Chem. 1972, 20, 266-270.
10. Blum, H.; Beier, H.; Gross, H. J. Improved silver staining of plant proteins, RNA, and DNA in polyacrylamide gels. Electrophoresis 1987, 8, 93-99.
11. Bordenave, M.; Goldberg, R. Purification and characterization of pectin methylesterases from mung bean hypocoltyl cell wall. Phytochemistry 1993, 33, 999-1003.
12. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 1976, 72, 248-254.
13. Castaldo, D.; Quagliuolo, L.; Servillo, L.; Ballestrieri, C.; Giovane, A. Isolation and characterization of pectin methylesterase from apple fruit. J. Food Sci. 1989, 54, 653-673.
14. Elkashif, M. E.; Huber, D. J. Enzymic hydrolysis of placental cell wall pectins and cell separation in watermelon (Citrullus lanatus) fruit exposed to ethylene. Physiol. Plant 1988, 73, 432-439.
15. Englard, S.; Seifter, S. Precipitation techniques. In Methods in Enzymology; Deutscher, M. P., Ed.; Academic Press: San Diego, CA (EEUU), 1990; Vol. 182.
16. Facteau, T. J.; Rowe, K. E.; Chestnut, N. E. Response of Bing and Lambert sweet cherry fruit to preharvest calcium chloride applications. HortScience 1987, 22, 271-273.
17. Fayyaz, A.; Asbi, B. A.; Ghazali, H. M.; Man, Y. B. C.; Jinap, S. Kinetics of papaya pectinesterase. Food Chem. 1995, 53, 129-135.
18. Gaffe, J.; Morvan, C.; Jauneau, A.; Demarty, M. Partial purification of flax cell wall pectin methylesterase. Phytochemistry 1992, 31, 761-765.
19. Gaffe, J.; Tieman, D. M.; Handa, A. K. Pectin methylesterase isoforms in tomato (Lycopersicon esculentum) tissues. Effects of expression of a pectin methylesterase antisense gene. Plant Physiol. 1994, 105, 199-203.
20. Giovane, A.; Quagliuolo, D.; Servillo, L.; Balestrieri, C. Pectin methyl esterase from Actinidia chinensis fruits. Phytochemistry 1990, 29, 2821-2823.
21. Glenn, G. M.; Poovaiah, B. W. Cuticular properties and postharvest calcium applications influence cracking of sweet cherries. J. Am. Soc. Hortic. Sci. 1989, 114, 781-788.
22. Glover, H.; Brady, C. Purification of three pectin esterases from ripe peach fruit. Phytochemistry 1994, 37, 949-955.
23. Goldberg, R. Changes in the properties of cell wall pectin methylesterase along the Vigna radiata hypocotyl. Physiol. Plant. 1984, 61, 58-63.
24. Hagerman, A. E.; Austin, P. J. Continuous spectrophotometric assay for plant pectin methyl esterase. J. Agric. Food Chem. 1986, 34, 440-444.
25. Heukeshoven, J.; Dernik, R. Simplified method for silver staining of proteins in polyacrilamide gels and the mechanism of silver staining. Electrophoresis 1985, 6, 103-112.
26. Javeri, H.; Wicker, L. Partial purification and characterization of peach pectinesterase. J. Food Biochem. 1991, 15, 241-252.
27. Laemmli, U. K. Cleavage of structural proteins during the assembly of head of bacteriophage T4. Nature 1970, 227, 680-685.
28. Lee, M.; Macmillan, J. D. Mode of action of pectic enzymes. I. Purification and certain properties of tomato pectinesterase. Biochemistry 1968, 7, 4005-4010.
29. Lidster, P. D.; Porrit, S. W.; Tung, M. A. Effects of a delay in storage and calcium chloride dip on surface disorder incidence in Van cherry. J. Am. Soc. Hortic. Sci. 1979, 104, 298-300.
30. Lin, T-P.; Liu, Ch.-Ch.; Chen, S.-W.; Wang, W.-Y. Purification and characterization of pectinmethylesterase from Ficus awkeotsang Makino Achenes. Plant Physiol. 1989, 91, 1445-1453.
31. Manjon, A.; Iborra, J. L.; Romero, C.; Canovas, M. Properties of pectinesterase and endo-D-polygalacturonase coimmobilized in a porous-glass support. Appl. Biochem. Biotechnol. 1992, 37, 19-31.
32. MAPA Norma de Calidad para Cerezas; Secretaría General Técnica del Ministerio de Agricultura, Pesca y Alimentación: Madrid, Spain, 1987.
33. Markovič, O.; Jörnvall, H. Pectinesterase. The primary structure of the tomato enzyme. Eur. J. Biochem. 1986, 158, 455-462.
34. Markovič, O.; Heinrichová, K.; Lenkey, B. Pectolytic enzymes from banana. Collect. Czech. Chem. Commun. 1975, 40, 769-774.
35. McFeeters, R. F.; Armstrong, S. A. Measurement of pectin methylation in plant cell walls. Anal. Biochem. 1984, 139, 212-217.
36. Moustacas, A.; Nari, J.; Borel, M.; Noat, G.; Ricard, J. Pectin methylesterase, metal ions in plant cell-wall extension. The role of metal ions in plant cell-wall extension. Biochem. J. 1991, 279, 351-354.
37. Nari, J.; Noat, G.; Ricard, J. Pectin methylesterase, metal ions and plant cell-wall extension. Hydrolysis of pectin by plant cell-wall pectin methylesterase. Biochem. J. 1991, 279, 343-350.
38. Pitkaenen, K.; Heikinheimo, R.; Pakkanen, R. Purification and characterization of Erwinia chrysanthemi B374 pectin methylesterase produced by Bacillus subtilis. Enzyme Microb. Technol. 1992, 14, 832-836.
39. Pressey, R.; Woods, F. M. Purification and properties of two pectinesterases from tomatoes. Phytochemistry 1992, 31, 1139-1142.
40. Randhawa, J. S.; Dhillon, B. S.; Bal, J. S.; Bhullar, J. S. Studies on the pectin methylesterase activity during cold storage of pathernakh pear. J. Food Sci. Technol. 1987, 24, 71-73.
41. Rexová-Benková, L.; Markovič, O. Pectic enzymes. Adv. Carbohydr. Chem. Biochem. 1976, 33, 323-385.
42. Rhodes, M. J. C. The maturation and ripening of fruit. In Senescence in Plants; Thimann, K. V., Ed.; CRC Press: London, UK, 1980.
43. Ricard, J.; Noat, G. Electrostatic effects on the dynamic of enzyme reactions at the surface of plant cells. 1. A theory of the ionic control of a complex multi-enzyme system. Eur. J. Biochem. 1986, 155, 183-190.
44. Sedmak, J. J.; Gossberg, S. A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G-250. Anal. Biochem. 1977, 79, 544-552.
45. Seymour, T. A.; Preston, J. F.; Wicker, L.; Lindsay, J. A.; Wei, Ch.; Marshall, M. R. Purification and properties of pectinesterases of marsh white grapefruit pulp. J. Agric. Food Chem. 1991a, 39, 1080-1085.
46. Seymour, T. A.; Preston, J. F.; Wicker, L.; Lindsay, J. A.; Wei, Ch.; Marshall, M. R. Stability of pectinesterases of marsh white grapefruit pulp. J. Agric. Food Chem. 1991b, 39, 1075-1079.
47. Taillan, E.; Ambid, C.; Pech, J. C.; Raynal, J. Demethylation of pectic substances: relationship to methylesterase activity during brine storage of cherries. J. Food Sci. 1992, 57, 682-685.
48. 2 brining. J. Food Sci. 1967, 32, 435-436.
49. Versteeg, C.; Rambouts, F. M.; Spaansen, C. H.; Pilnik, W. Thermostability and orange juice cloud destabilizing properties of multiple pectinesterase from orange. J. Food Sci. 1980, 45, 969-972.
50. Wegrzyn, T. F.; McRae, E. A. Pectinesterase, polygalacturonase, and β-galactosidases during softening of ethylenetreated kiwi fruit. HortScience 1992, 27, 900-902.