Determination of Cyanogenic Glycosides in Flaxseed by Barbituric Acid-Pyridine, Pyridine-Pyrazolone, and High-Performance Liquid Chromatography Methods

Journal of Agricultural and Food Chemistry

Volumn 44, Issue 10, 1996, Pages 3178-3181

  Kobaisy, M ^a   Oomah, B D ^b   Mazza, G ^a  

^a SUMMERLAND RESEARCH AND DEVELOPMENT CENTRE   (Canada)

^b AGRICULTURE AND AGRI FOOD CANADA   (Canada)

Author keywords

Barbituric acid pyridine; Crude enzyme extract; Cyanogenic glycosides; Flaxseed; HPLC; Linum usitatissimum; Method comparison

Indexed keywords

LINUM USITATISSIMUM;

EID: 0001260056     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf950838j     Document Type: Article

References (21)

1. Amarowicz, R.; Wanasundara, P. K. J. P. D.; Shahidi, F. TLC separation of linamarin, linustatin and neolinustatin. Nahrung 1993, 37, 88-90.
2. Bhatty, R. S. Further compositional analyses of flax: mucilage, trypsin inhibitors and hydrocyanic acid. J. Am. Oil Chem. Soc. 1993, 70, 899-904.
3. Brimer, L.; Christensen, S. G.; Molgard, P.; Nartey, P. Determination of cyanogenic compounds by thin-layer chromatography. 1. A densitometric method for quantification of cyanogenic glycosides, employing enzyme preparations (β-glucuronidase) from Helix pomatia and picrate-impregnated ion-exchange sheets. J. Agric. Food Chem. 1983, 31, 78-793.
4. Chadha, R. K.; Lawrence, J. F.; Ratnayake, W. M. N. Ion Chromatographic determination of cyanide released from flaxseed under autohydrolysis conditions. Food Addit. Contam. 1995, 12, 527-533.
5. Conn, E. E. β-glycosidases in plants: substrate specificity. In β-glucosidases: Biochemistry and Molecular Biology; Esen, A., Ed.; American Chemical Society: Washington, DC, 1994; pp 15-26.
6. Cooke, R. D. An enzymatic assay for the total cyanide content of cassava (Manilot esculenta Crantz). J. Sci. Food Agric. 1978, 29, 345-352.
7. Cui, W.; Mazza, G.; Oomah, B. D.; Biliaderis, C. G. Optimization of an aqueous extraction process for flaxseed gum by response surface methodology. Lebensm.-Wiss. Technol. 1994, 27, 363-369.
8. Cunnane, C. S.; Thompson, L. U. Flaxseed in Human Nutrition; AOCS Press: Champaign, IL, 1995; p 384.
9. Fan, T. W.-M.; Conn, E. E. Isolation and characterization of two cyanogenic β-glucosidases from flax seeds. Arch. Biochem. Biophys. 1985, 243, 361-373.
10. Glew, R. H.; Gopalan, V.; Forsyth, G. W.; VanderJagt, D. J. The mammalian cytosolic broad-specificity β-glucosidase. In β-glucosidases: Biochemistry and Molecular Biology; Esen., A., Ed.; American Chemical Society: Washington, DC, 1994; pp 83-112.
11. Harris, J. R.; Merson, G. H. J.; Hardy, M. J.; Curtis, D. J. Determination of cyanide in animal feeding stuffs. Analyst 1980, 105, 974-980.
12. Jenkins, D. J. A. Incorporation of flaxseed or flaxseed components into cereal foods. In Flaxseed in Human Nutrition; Cunnane, S. C., Thompson, L. U., Eds.; AOCS Press: Champaign, IL, 1995; pp 281-294.
13. Kolodziejczyk, P. P.; Fedec, P. Processing flaxseed for human consumption. In Flaxseed in Human Nutrition; Cunnane, S. C., Thompson, L. U., Eds.; AOCS Press: Champaign, IL, 1995; pp 261-280.
14. Mazza, G.; Oomah, B. D. Flaxseed, dietary fiber and cyanogens. In Flaxseed in Human Nutrition; Cunnane, S. C., Thompson, L. U., Eds.; AOCS Press: Champaign, IL, 1995; pp 56-81.
15. Nambisan, B.; Sundaresan, S. Spectrophotometric determination of cyanoglucosidases in cassava. J. Assoc. Off. Anal. Chem. 1984, 67, 631-643.
16. O'Brien, G. M.; Taylor, A. J.; Poulter, N. H. Improved enzymic assay for cyanogens in fresh and processed cassava. J. Sci. Food Agric. 1991, 56, 277-289.
17. Oomah, B. D.; Mazza, G.; Kenaschuk, E. O. Cyanogenic compounds in flaxseed. J. Agric. Food Chem. 1992, 40, 1346-1348.
18. Oomah, B. D.; Kenaschuk, E. O.; Mazza, G. Safety considerations of flaxseed and flaxseed components. In Proceedings of the 55th Flax Institute of the United States; Flax Institute: Fargo, ND, 1994; pp 178-182.
19. Palmer, I. S. Effects of flaxseed on selenium toxicity. In Flaxseed in Human Nutrition; Cunnane, S. C., Thompson, L. U., Eds.; AOCS Press: Champaign, IL, 1995; pp 165-173.
20. SAS Institute. SAS/STAT User's Guide, version 6, 4th ed.; SAS Institute: Cary, NC, 1990.
21. Schilcher, H.; Wilkens-Sauter, M. Quantitative determination of cyanogenic glycosides in Linum usitatissimum using HPLC. Fette, Seife, Anstrichm. 1986, 8, 287-290.