Modified method for competitive reverse transcription polymerase chain reaction for rapid and automated quantitation of messenger RNA in multiple samples

Molecular Diagnosis

Volumn 2, Issue 4, 1997, Pages 235-240

  Vats, Abhay ^a   Katayama, Hajime ^a   Kim, Youngki ^a   Mauer, Michael ^a   Fish, Alfred J ^a   McGlennen, Ronald C ^a  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

Author keywords

Competitive rt pcr; Fluorescent primers; mRNA quantitation; Polymerase chain reaction

Indexed keywords

EID: 0001238113     PISSN: 10848592     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1084-8592(97)80034-3     Document Type: Article

References (3)

1. dementi M, Menzo S, Bagnarelli P, Manzin A, Valenza A, Varaldo PE: Quantitative PCR and RTPCR in virology. PCR Methods Appl 1993;2:191-196
2. Gilland G, Perrin S, Blanchard K, Bunn HF: Analysis of cytokine mRNA and DNA: detection and quantitation by the polymerase chain reaction. Proc Natl Acad Sci USA 1990;87:2725-2729
3. Clementi M, Bagnarelli P, Manzin A, Menzo S: Competitive polymerase chain reaction and analy-sis of viral activity at the molecular level. Genet Anal Tech Appl 1994;11:l-6