Surface site heterogeneity and lateral interactions in multipoint protein adsorption

Journal of Physical Chemistry

Volumn 100, Issue 12, 1996, Pages 5134-5139

  Johnson, R D ^a,b   Wang, Z G ^a   Arnold, F H ^a  

^a California Institute of Technology ^*  (United States)

^b GENENTECH INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0001202917     PISSN: 00223654     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp9523682     Document Type: Article

References (29)

1. Jennissen, H. P. J. Chromatogr. 1978, 159, 71-83.
2. Dowd, V.; Yon, R. J. J. Chromatogr. 1992, 627, 145-151.
3. Gill, D. S.; Roush, D. J.; Willson, R. C. J. Colloid Interface Sci. 1994, 167, 1-7.
4. Todd, R. J.; Johnson, R. D.; Arnold, F. H. J. Chromatogr. A 1994, 662, 13-26.
5. Johnson, R. D.; Arnold, F. H. Biochim. Biophys. Acta 1995, 1247, 293-297.
6. Tompkins, F. C. Chemisorption of Gases on Metals, Academic Press: San Francisco, CA, 1978.
7. Levitzki, A. Receptors: A Quantitative Approach, Benjamin/ Cummings: Menlo Park, CA, 1984.
8. Mallik, S.; Plunkett, S.; Dhal, P. K.; Johnson, R. D.; Pack, D.; Shnek, D.; Arnold, F. H. New J. Chem. 1994, 18, 299-304.
9. Johnson, R. D.; Arnold, F. H. Biotechnol. Bioeng. 1995, 48, 437-443.
10. Vunnum, S.; Gallant, S.; Kim, Y.; Cramer, S. M. Chem. Eng. Sci., in press.
11. Johnson, R. D.; Todd, R. J.; Arnold, F. H. J. Chromatogr. A 1996, 725, 225-235.
12. Drager, R. R.; Regnier, F. E. J. Chromatogr. 1986, 359, 147-155.
13. Velayudhan, A.; Horvath, C. J. Chromatogr. A 1994, 663, 1-10.
14. Gerstner, J. A.; Bell, J. A.; Cramer, S. M. Biophys. Chem. 1994, 52, 97-106.
15. 5 An additional difficulty comes from the fact that the adsorbate can bind to two or more surface sites. An elegant discussion of the multisite effects for irreversible adsorption of homogeneous objects has been given by Jin et al. (Jin, X.; Wang, N.-H. L.; Tarjus, G.; Talbot, J. J. Phys. Chem. 1993, 97, 4256-4258). It is not clear, however, how this treatment can be generalized to the case of reversible adsorption of objects that have imbedded inhomogeneity (e.g., the distribution of histidines on a protein).
16. 0).
17. The lattice size should not be taken literally as the true lattice size of the surface, but rather as some effective size corresponding to the "sphere of influence" of a binding site.
18. At very low p, eq 6 is not expected to be valid because of possible restrictions in translation, rotation, etc., due to artifacts arising from dividing the surface into discrete cells.
19. Bolis, V.; Morterra, C.; Fubini, B.; Ugliengo, P.; Garrone, E. Langmuir 1993, 9, 1521-1528.
20. Braag, W. L.; Williams, E. J. Proc. R. Soc. A 1934, 145, 699-730.
21. pm - Zw).
22. Yang, C.-H. J. Phys. Chem. 1993, 97, 7097-7101.
23. For simplicity we have presumed an imidazole solution standard state of 1 M.
24. Arnold, F. H.; Schofield, S. A.; Blanch, H. W. J. Chromatogr. 1986, 335, 1-12.
25. p).
26. Wu, D.; Walters, R. J. Chromatogr. 1992, 598, 7-13.
27. p = 3.8 μmol protein/mL TSK (see Figure 4).
28. 19 as a result it is also temperature-dependent.
29. 4