HPLC analysis of anomeric formation and cleavage pattern by chitinolytic enzyme

Bioscience, Biotechnology and Biochemistry

Volumn 62, Issue 8, 1998, Pages 1643-1646

  Koga, Daizo ^a   Yoshioka, Takanori ^a   Arakane, Yasuyuki ^b  

^a YAMAGUCHI UNIVERSITY   (Japan)

^b Research Laboratory   (Japan)

Author keywords

Chitinase; Chitinolytic enzyme; Lysozyme; N acetylchitooligosaccharide

Indexed keywords

EID: 0001199167     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.62.1643     Document Type: Article

References (9)

1. Terwisscha van Scheltinga, A. C., Kalk, K. H., Beintema, J. J., and Dijkstra, B. W., Crystal structures of hevamine, a plant defence protein with chitinase and lysozyme activity, and its complex with an inhibitor. Structure, 2, 1181-1189 (1994).
2. Terwisscha van Scheltinga, A. C., Armand, S., Kalk, K. H., Isogai, A., Henrissat, B., and Dijkstra, B. W., Stereochemistry of chitin hydrolysis by a plant chitinase/lysozyme and x-ray structure of a complex with allosamidin: evidence for substrate assisted catalysis. Biochemistry, 34, 15619-15623 (1995).
3. Tews, I., Terwisscha van Scheltinga, A. C., Perrakis, A., Wilson, K S., and Dijkstra, B. W., Substrate-assisted catalysis unifies two families of chitinolytic enzymes. J. Am. Chem. Soc., 119, 7954-7959 (1997).
4. Armand, S., Tomita, H., Heyraud, A., Gey, C., Watanabe, T., and Henrissat, B., Stereochemical course of the hydrolysis reaction catalyzed by chitinases A1 and D from Bacillus circulans WL-12. FEBS lett., 343, 177-180 (1994).
5. Fukamizo, T., Koga, D., and Goto, S., Comparative biochemistry of chitinases-anomeric form of the reaction products. Biosci. Biotech. Biochem., 59, 311-313 (1995).
6. Koga, D., Tsukamoto, T., Sueshige, N., Utsumi, T., and Ide, A., Kinetics of chitinase from Yam, Dioscorea opposita THUNB. Agric. Biol. Chem., 53, 3121-3126 (1989).
7. Tsukamoto, T., Koga, D., Ide, A., Ishibashi, T., Horino-Matsushige, M., Yagishita, K., and Imoto, T., Purification and some properties of chitinases from Yam, Dioscorea opposita THUNB. Agric. Biol. Chem., 53, 3121-3126 (1984).
8. Dahlquist, F. W., Borders, C. L., Jacobson, G., and Raftery, M. A., The spectrospecificity of human, hen, and papaya lysozymes. Biochemistry, 8, 694-700 (1969).
9. Iseli, B., Armand, S., Boiler, T., Neuhaus, J. M., Henrissat, B., Plant chitinases use two different hydrolytic mechanisms. FEBS Lett., 382, 186-188 (1996).