Molecular cloning and expression of Pfu DNA polymerase gene and its application in long-distance PCR

Chinese Science Bulletin

Volumn 43, Issue 10, 1998, Pages 863-867

  Li, Bing ^a   Gu, Cheng ^a   Zhao, Jindong ^a  

^a PEKING UNIVERSITY   (China)

Author keywords

Expression; Long distance PCR; Pfu enzyme

Indexed keywords

ESCHERICHIA COLI; PYROCOCCUS FURIOSUS;

EID: 0001089242     PISSN: 10016538     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF03182756     Document Type: Article

References (10)

1. Barnes, W. M., PCR amplification of upto 35 kb DNA with high fidelity and high yield from bacteriophage template, Proc. Natl. Acad. Scie. USA, 1994, 91: 2 216.
2. Mattila, P., Korpela, J., Tenkanen, T. et al., Fidelity of DNA synthesis by the thermococcus litoralis DNA polymerase - an extremely heat stable enzyme with proofreading activity, Nucl. Aci. Res., 1991, 19: 4967.
3. Ausubel, F. M., Brent, R., Kingston, R. E. et al., Short Protocols in Molecular Biology, 3rd ed., Boston: John Willy and Sons, Inc., 1995.
4. Buguess, R. B., Use of polyethylenemine in purification of DNA binding proteins, Meth Enzymol., 1991, 208: 1.
5. Cheng, S., Chang, S. Y., Respess, R., Long PCR, Nature, 1994, 369: 684
6. Uemori, T., Ishhino, Y., Yoh, H. et al., Organization and nucleotide sequence of the DNA polymerase gene from the archaeon Pyrococcus furiosus, Nucl. Aci. Res., 1993, 21: 259.
7. Foord, O. S., Rose, E. A., Long-distance PCR, PCR Methods Appl., 1994, 3(6): S149-61.
8. Gustafson, C. E., Alm, R. A., Trust, T. J., Effect of heat denaturation of target DNA on the PCR amplification, Gene, 1993, 123: 214.
9. Cheng, S., Fockler, C., Barnes, W. M. et al., Effective amplification of long target from cloned inserts and human genomic DNA, Proc. Natl. Acad. Scie. USA, 1994, 91: 5 695.
10. Cheng, S., Higuchi, R., Stoneking, M., Complete mitochondrial genome amplification, Nat. Genet., 1994, 7: 350.