Microstructure of Indirectly and Directly Heated Ultra-high-temperature (UHT) Processed Milk Examined using Transmission Electron Microscopy and Immunogold Labelling

LWT

Volumn 32, Issue 8, 1999, Pages 486-494

  Hillbrick, G C ^a   McMahon, D J ^b   McManus, W R ^a  

^a UTAH STATE UNIVERSITY   (United States)

^b UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Fat globules; homogenization; immunogold labelling; milk; transmission electron microscopy; UHT

Indexed keywords

EID: 0001017748     PISSN: 00236438     EISSN: None     Source Type: Journal    
DOI: 10.1006/fstl.1999.0586     Document Type: Article

References (32)

1. VARNAM A. H., SUTHERLAND J. P. Milk and Milk Products. Technology, Chemistry and Microbiology. 1994;Chapman and Hall, London. p. 42-102.
2. SAWYER W. H. Complex between β -lactoglobulin and κ -casein. A review. Journal of Dairy Science. 52:1969;1347-1355.
3. ZITTLE C. A., THOMPSON M. P., CUSTER J. H., CERBULIS J. κ -Casein-β -lactoglobulin interaction in solution when heated. Journal of Dairy Science. 45:1962;807-810.
4. CORREDIG M., DALGLEISH D. G. Effect of different heat treatments on the strong binding interactions between whey protein and milk fat globules in whole milk. Journal of Dairy Research. 63:1996;441-449.
5. KIM H.-H. Y., JIMENEZ-FLORES R. Heat-induced interactions between the proteins of milk fat globule membrane and skim milk. Journal of Dairy Science. 78:1995;24-35.
6. HOULIHAN A. V., GODDARD P. A., NOTTINGHAM S. M., KITCHEN B. J., MASTERS C. J. Interactions between the bovine milk fat globule membrane and skim milk components on heating whole milk. Journal of Dairy Research. 59:1992;187-185.
7. ZADOW J. G., HARDHAM J. F. The effect of single or triple piston homogenization on the fat globule distribution of milk. Australian Journal of Dairy Technology. 39:1984;36-40.
8. ALLEYNE M. C., MCMAHON D. J., YOUSSEF N. N., HEKMAT S. An apparatus for a new microcube encapsulation of fluid milk in preparation for transmission electron microscopy. Food Structure. 12:1993;21-30.
9. ALLEYNE M. C. Fate of β -lactoglobulin, α -lactalbumin, and casein proteins in ultrafiltered concentrated milk after ultra-high temperature processing: 1994;Utah State University, Logan.
10. HUMBEL B. M., SIBON O. C. M., STIERHOF Y. D., SCHWARZ H. Ultra-small gold particles and silver enhancement as a detection system in immunolabeling and in situ hydridization experiments. Journal of Histochemistry and Cytochemistry. 43:1995;735-737.
11. YOUSSEF N. N. Practical Electron Microscopy. 1985;Utah State UniversityDepartment of Biology, Logan. p. 105-110.
12. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology. 17:1963;208-213.
13. ZADOW J. G. Studies on the ultra-heat treatment of milk. Part 1 - comparison of direct and indirect heating of whole milk. Australian Journal of Dairy Technology. 24:1969;44-49.
14. BENDAYAN M., ZOLLINGER M. Ultrastructural localization of antigenic sites on osmium-fixed tissues applying the protein A-gold technique. Journal of Histochemistry and Cytochemistry. 31:1983;101-109.
15. WALSTA P., OORTWIJN H. Estimating globule-size distribution of oil-in-water emulsions by Coulter counter. Journal of Colloid and Interface Science. 29:1969;424-431.
16. DALGLEISH D. G., TOSH S. M., WEST S. Beyond homogenization: the formation of very small emulsion droplets during the processing of milk by a Microfluidizer. Netherlands Milk & Dairy Journal. 50:1996;135-148.
17. DALGLEISH D. G., WEST S. J., HALLETT F. R. The characterization of small emulsion droplets made from milk proteins and triglyceride oil. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 123-124:1997;145-153.
18. HENSTRA S., SCHMIDT D. G. On the structure of the fat-protein complex in homogenised cow's milk. Netherlands Milk & Dairy Journal. 24:1970;45-51.
19. DARLING D. F., BUTCHER D. W. Milk-fat globule membrane in homogenized cream. Journal of Dairy Research. 45:1978;197-208.
20. DALGLEISH D. G., ROBSON E. W. Centifugal fractionation of homogenized milks. Journal of Dairy Research. 52:1985;539-546.
21. WALSTRA P., JENNESS R. Dairy Chemistry and Physics. 1982;John Wiley & Sons, New York.
22. OGDEN L. V., WALSTRA P., MORRIS H. A. Homogenization-induced clustering of fat globules in cream and model systems. Journal of Dairy Science. 59:1976;1727-1737.
23. OORTWIJN H., WALSTRA P. The membranes of recombined fat globules. 2. Composition. Netherlands Milk & Dairy Journal. 33:1979;134-154.
24. OORTWIJN H., WALSTRA P., MULDER H. The membranes of recombined fat globules. 1. Electron microscopy. Netherlands Milk & Dairy Journal. 31:1977;134-147.
25. MCPHERSON A. V., DASH M. C., KITCHEN B. J. Isolation and composition of milk fat globule membrane material. II. From homogenized and ultra heat treated milks. Journal of Dairy Research. 51:1984;289-297.
26. JANG H. D., SWAISGOOD H. E. Disulfide bond formation between thermally denatured β -lactoglobulin and κ -casein in casein micelles. Journal of Dairy Science. 73:1990;900-904.
27. AOKI T., SUZUKI H., IMAMURA T. Some properties of soluble casein in heated concentrated whey protein-free milk. Milchwissenschaft. 30:1975;30-35.
28. SINGH H., FOX P. F. Heat stability of milk: the mechanism of stabilization by formaldehyde. Journal of Dairy Research. 52:1985;65-76.
29. SINGH H., FOX P. F. Heat stability of milk: role of β -lactoglobulin in the pH-dependent dissociation of micellarκ -casein. Journal of Dairy Research. 54:1987;509-521.
30. DALGLEISH D. G., BANKS J. M. The formation of complexes between serum proteins and fat globules during heating of whole milk. Milchwissenschaft. 46:1991;75-78.
31. WALSTA P., OORTWIJN H. The membranes of recombined fat globules. 3. Mode of formation. Netherlands Milk & Dairy Journal. 36:1982;103-113.
32. SHARMA S. K., DALGLEISH D. G. Interactions between milk serum proteins and synthetic fat globule membrane during heating of homogenized whole milk. Journal of Agricultural and Food Chemistry. 41:1993;1407-1412.