Electronic spectroscopy of gold(I) Pseudomonas aeruginosa azurin derivatives

Inorganic Chemistry

Volumn 35, Issue 11, 1996, Pages 3434-3435

  Mark McCleskey, T ^a   Mizoguchi, Tadashi J ^a   Richards, John H ^a   Gray, Harry B ^a  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0000962341     PISSN: 00201669     EISSN: None     Source Type: Journal    
DOI: 10.1021/ic951055i     Document Type: Article

References (27)

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17. A gold derivative of azurin has been reported previously: Otiko, G.; Sadler, P. J. Biochem. Soc. Trans. 1981, 9, 445-446.
18. Crystal structures of the WT protein have been determined: (a) Adman, E. T., Jensen. L. H. Isr. J. Chem. 1981, 21, 8-12.
19. (b) Nar, H.; Messerschmidt, A.; Huber, R.; van de Kamp, M.: Canters, G. W. J. Mol. Biol. 1991, 221, 765-772.
20. Chang, T. K.; Iverson, S. A.; Rodngues, C. G.; Kiser, C. N.; Lew, A. Y. C.; Germanas, J. P.; Richards, J. H. Proc. Natl. Acad. Sci. U.S.A. 1991, 88, 1325-1329.
21. Karlsson, B. G.; Nordling, M.: Pascher, T.; Tsai, L.-C ; Sjölin, L.; Lundberg, L. G. Protein Eng. 1991, 4, 343-349
22. Di Bilio, A. J.; Chang, T. K.: Malmström, B. G.; Gray, H. B.; Karlsson, B. G.; Nordling, M.; Pascher, T.; Lundberg, L. G. Inorg. Chim. Acta 1992, 198-200, 145-148.
23. Piccioli, M.; Luchinat, C.; Mizoguchi; T. J.; Ramirez, B. E.; Gray, H. B.; Richards, J. H. Inorg. Chem. 1995, 34, 737-742.
24. Bailey, J. A.; Hill, M. G.; Marsh, R. E.; Miskowski, V M.; Schaefer, W. P.; Gray, H. B. Inorg. Chem. 1995, 34, 4591-4599.
25. It is likely that Au(I) binds strongly to the Cys112 sulfur in the active site. Attempts to reconstitute Cys112Asp P. aeruginosa azurin (Mizoguchi, T. J.; Di Bilio, A. J.; Gray, H. B.; Richards, J. H. J. Am. Chem. Soc. 1992, 114, 10076-10078) with Au(I) failed. Samples of Au(I)-treated Cys112Asp azurin exhibited an absorption spectrum indistinguishable from that of the apo protein; and these samples could be reconstituted with Cu(II).
26. 3E″) state of trigonal planar Au(I) is strongly emissive.
27. Emission from a Au(I)-substituted metallothionein has been reported previously: Stillman, M. J.; Zelazowski, A- J.; Szymanska, J.; Gasyna, A. Inorg. Chim. Acta 1989, 161, 275-279.