Partial purification and characterization of a diurnally fluctuating novel endoamylase from Arabidopsis thaliana leaves

Plant Physiology and Biochemistry

Volumn 35, Issue 12, 1997, Pages 907-913

  Kakefuda, Genichi ^a,b   Preiss, Jack ^a  

^a Michigan State University   (United States)

^b PFIZER INC   (United States)

Author keywords

Amylase; Arabidopsis thaliana; Leaf; Mutants; Starch degradation; Transitory starch

Indexed keywords

EID: 0000955301     PISSN: 09819428     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Beck E. and Ziegler P., 1989. Biosynthesis and degradation of starch in higher plants. Annu. Rev. Plant Physiol. Plant Mol. Biol., 40, 95-117.
2. Beutler H.-O., 1981. Starch. In Methods of enzymatic analysis, 6, Bergmeyer ed., 2-10.
3. Caspar T., Lin T.-P., Kakefuda G., Benbow L., Preiss J. and Somerville C., 1991. Mutants of Arabidopsis deficient in starch degradation. Plant Physiol., 95, 1181-1188.
4. Doehlert D. C. and Duke S.H., 1983. Specific determination of cc-amylase activity in crude plant extracts containing β-amylase. Plant Physiol., 71, 229-234.
5. Dunn G., 1974. A model for starch breakdown in higher plants. Phytochemistry, 13, 1341-1346.
6. Hammond J. B. W. and Burton K. S., 1983. Leaf starch metabolism during growth of pepper (Capsicum annuum) plants. Plant Physiol., 73, 61-65.
7. Kakefuda G. and Duke S. H., 1984. Electrophoretic transfer as a technique for the detection and identification of plant amylolytic enzymes in polyacrylamide gels. Plant Physiol., 75, 278-280.
8. Kakefuda G. and Duke S. H., 1989. Characterization of pea chloroplast D-enzyme (4-α-D-glucanotransferase). Plant Physiol., 91, 136-143.
9. Kakefuda G., Duke S. H. and Hostak M. S., 1986. Chloroplast and extrachloroplastic starch-degrading enzymes in Pisum sativum L. Planta, 168, 175-182.
10. Lin T.-P., Spilatro S. R. and Preiss J., 1988. Subcellular localization and characterization of amylases in Arabidopsis leaf. Plant Physiol., 86, 251-259.
11. Marshall J. J. and Whelan W. J., 1971. Detection of endo-acting carbohydrates, particularly in the presence of exoenzymes acting on the same substrate. Anal. Biochem., 43, 316-321.
12. Monroe J. D. and Preiss J., 1990. Purification of a β-amylase that accumulates in Arabidopsis thaliana mutants defective in starch metabolism. Plant Physiol., 94, 1033-1039.
13. Nelson N., 1944. A photometric adaptation of the Somogyi method for the determination of glucose. J. Biol. Chem., 153, 375-380.
14. Pongratz P. and Beck E., 1978. Diurnal oscillation of amylolytic activity in spinach chloroplasts. Plant Physiol., 62, 687-689.
15. Preiss J., 1982. Regulation of the biosynthesis and degradation of starch. Annu. Rev. Plant Physiol., 33, 431-454.
16. Preiss J. and Sivak M. N., 1996. Starch synthesis in sinks and sources. In Photoassimilate distribution in plants and crops: Souce-sink relationships, Zamski E., and Schaffer A.A., ed., Marcel Dekker Inc., New York, 63-96.
17. Rick W. and Stegbauer H. P., 1974. α-Amylase, measurement of reducing groups. Methods Enzym. Anal., 2, 884-890.
18. Steup M., Robenek H. and Melkonian M., 1983. In vitro degradation of starch granules isolated from spinach chloroplasts. Planta, 158, 428-436.
19. Stitt M., Bulpin P. V. and ap Rees T. 1978. Pathway of starch breakdown in photosynthetic tissues of Pisum sativum. Biochim. Biophys. Acta, 544, 200-214.
20. Stitt M. and Heldt H. W., 1981. Physiological rates of starch beakdown in isolated spinach chloroplasts. Plant Physiol., 68: 755-761.
21. Sturgeon R.J., 1980. Enzymic determination of D-glucose, D-fructose, and D-mannose. In Methods in carbohydrate chemistry 8, BeMiller W. ed., 135-137.
22. Sun Z. and Henson C. A., 1990. Degradation of native starch granules by barley α-glucosidases. Plant Physiol., 94, 320-327.
23. Vieweg G. H. and de Fekete M. A. R., 1977. Diurnal fluctuation of amylase activity in Kalanchoe daigremontiana leaves. Z Pflanzenphysiol., 81, 74-79.
24. Ziegler P., 1988. Partial purification and characterization of the major endoamylase of mature pea leaves. Plant Physiol. 86, 659-666.
25. Ziegler P., 1995. Carbohydrate degradation during germination. In Seed development and germination, Kigel J. and Galili G., ed., Dekker M., Inc. New York, 447-474.