Effect of denaturant and cosolvents on the stability of wheat germ lipase

Journal of Agricultural and Food Chemistry

Volumn 44, Issue 3, 1996, Pages 736-740

  Rajeshwara, A N ^a   Prakash, V ^a  

^a CENTRAL FOOD TECHNOLOGICAL RESEARCH INSTITUTE   (India)

Author keywords

Activity; Denaturation; Hydration; Thermal stabilization; Wheat germ lipase

Indexed keywords

TRITICUM AESTIVUM;

EID: 0000895720     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf950335d     Document Type: Article

References (32)

1. Arakawa, T.; Timasheff, S. N. Stabilization of protein structure by sugars. Biochemistry 1982a, 21, 6536-6544.
2. Arakawa, T.; Timasheff, S. N. Preferential interaction of proteins with salts in concentrated solutions. Biochemistry 1982b, 21, 6545-6552.
3. Arakawa, T.; Timasheff, S. N. Theory of protein solubility. Methods Enzymol. 1985, 114, 49-77.
4. Back, J. F.; Okenfull, D.; Smith, M. B. Increased thermal stability of proteins in the presence of sugars and polyols. Biochemistry 1979, 18, 5191-5196.
5. Borgstrom, B.; Brockman, H. L. Lipases; Elsevier Science Publishers: Amsterdam, 1984.
6. Brockerhoff, H.; Jensen, R. Lipolytic Enzymes; Academic Press: New York, 1974.
7. Casassa, E. F.; Eisenberg, H. Partial specific volumes and refractive index increments in multicomponent systems. J. Phys. Chem. 1961, 65, 427-433.
8. Casassa, E. F.; Eisenberg, H. Thennodynamic analysis of multi-component solutions. Adv. Protein Chem. 1964, 19, 287-395.
9. Gekko, K.; Timasheff, S. N. Mechanism of protein stabilization by glycerol: preferential hydration in glycerol-water mixtures. Biochemistry 1981, 20, 4667-4676.
10. Gerlsma, S. Y.; Stuur, E. R. Effect of polyhydric and monohydric alcohols on the heat-induced reversible denaturation of lysozyme and ribonuclease. Int. J. Pept. Protein Res. 1972, 4, 377-383.
11. Huang, A. H. C. Plant Lipases. In Lipases; Borgstrom, B., Brockman, H. L., Eds.; Elsevier Science Publishers: Amsterdam, 1984.
12. Jencks, W. P. Catalysis in Chemistry and Enzymology; McGraw-Hill: New York, 1969.
13. Joly, M. A. Physico-Chemical Approach to the Denaturation of Proteins, Academic Press: London, 1965.
14. Kuntz, I. D. Hydration of macromolecules. III. Hydration of polypeptides. J. Am. Chem. Soc. 1971, 93, 514-516.
15. Lee, J. C.; Timasheff, S. N. Partial specific volumes and interactions with solvent components of proteins in guanidine hydrochloride. Biochemistry 1974a, 13, 257-265.
16. Lee, J. C.; Timasheff, S. N. The calculation of partial specific volumes of proteins in guanidine hydroehloride. Arch. Biochem. Biophys. 1974b, 165, 268-273.
17. Lee, J. C.; Timasheff, S. N. The stabilization of proteins by sucrose. J. Biol. Chem. 1981, 256, 7193-7201.
18. Lee, J. C.; Frignon, R. P.; Timasheff, S. N. Structural stability of calf brain microtubule protein. Ann. N. Y. Acad. Sci. 1975, 253, 284-291.
19. Prakash, V. Partial specific volumes and interactions with solvent components of alpha-globulin from Sesamum indicum L. in urea and guanidine hydrochloride. J. Biosci. 1982, 4, 347-359.
20. Prakash, V.; Timasheff, S. N. The calculation of partial specific volumes of proteins in 8 M urea solution. Anal. Biochem. 1981, 117, 330-335.
21. Prakash, V.; Timasheff, S. N. Calculation of partial specific volumes of proteins in 8 M urea solution. Methods Enzymol. 1985, 117, 53-60.
22. Rajendran, S.; Sudhindra Rao, K.; Prakash, V. Effect of pH in the acidic region on the structural integrity of lipase from wheat germ. Indian J. Biochem. Biophys. 1990, 27, 300-310.
23. Rajeshwara, A. N.; Prakash, V. Interaction of guanidine hydrochloride and guanidine thiocyanate with wheat germ lipase. Indian J. Biochem. Biophys. 1994a, 31, 315-321.
24. Rajeshwara, A. N.; Prakash, V. Structural stability of lipase from wheat germ. Int. J. Peptide Protein Res. 1994b, 44, 435-440.
25. Robinson, P. R.; Jencks, W. P. The effect of compounds of the urea-guanidinium class on the activity coefficients of acetyltetraglycine ethyl ester and related compounds. J. Am. Chem. Soc. 1965, 87, 2462-2470.
26. Scatchard, G. Physical chemistry of protein solutions. I. Derivation of the equations for the osmotic pressure. J. Am. Chem. Soc. 1946, 68, 2315-2319.
27. Simpson, R. B.; Kauzmann, W. Kinetics of protein denaturation. I. The behavior of the optical rotation of ovalbumin in urea solutions. J. Am. Chem. Soc. 1953, 75, 5139-5152.
28. Singer, T. P.; Hofstee, B. H. J. Studies on wheat germ lipase. I. Methods of estimation, purification and general properties of the enzyme. Arch. Biochem. Biophys. 1948, 18, 229-244.
29. Stockmayer, W. H. Light scattering in multicomponent systems. J. Chem. Phys. 1950, 18, 58-61.
30. Sudhindra Rao, K.; Rajendran, S.; Rajeshwara, A. N.; Prakash, V. Structural stability of lipase from wheat germ in alkaline pH. J. Protein Chem. 1991, 10, 291-299.
31. Tanford, C. Protein denaturation. Part A: Characterization of the denatured state, and Part B: the transition from native to denatured state. Adv. Protein Chem. 1968, 23, 121-282.
32. Timasheff, S. N.; Lee, J. C.; Pittz, E. P.; Tweedy, N. J. The interaction of tubulin and other proteins with structure-stabilizing solvents. Colloid Interface Sci. 1976, 55, 658-663.