Effects of high-intensity strength training on steady-state myosin heavy chain isoform Mrna expression

Journal of Exercise Physiology Online

Volumn 3, Issue 4, 2000, Pages 13-25

  Willoughby, Darryn S ^a   Pelsue, Stephen ^b  

^a TEXAS CHRISTIAN UNIVERSITY   (United States)

^b UNIVERSITY OF SOUTHERN MAINE   (United States)

Author keywords

Pre translational; RT PCR; Strength; Translational

Indexed keywords

DNA FRAGMENT; MESSENGER RNA; MUSCLE PROTEIN; MYOSIN HEAVY CHAIN; MYOSIN HEAVY CHAIN TYPE I; MYOSIN HEAVY CHAIN TYPE IIA; MYOSIN HEAVY CHAIN TYPE IIX; UNCLASSIFIED DRUG;

ADULT; ARTICLE; CONTROLLED STUDY; DNA TEMPLATE; GENE EXPRESSION REGULATION; HUMAN; HUMAN EXPERIMENT; HUMAN TISSUE; MALE; MUSCLE CONTRACTION; MUSCLE EXERCISE; MUSCLE MASS; MUSCLE STRENGTH; MUSCLE TRAINING; NORMAL HUMAN; PHYSICAL ACTIVITY; QUANTITATIVE ANALYSIS; RANDOMIZATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SKELETAL MUSCLE; STEADY STATE; VASTUS LATERALIS MUSCLE;

EID: 0000776745     PISSN: 10979751     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Mahdavi V, Izumo S, Nadal-Ginard B. Developmental and hormonal regulation of sarcomeric myosin heavy gene family. Circul Res 1987;60:804-814.
2. Perisamy M, Gregory P, Martin B, Stirewalt W. Regulation of myosin heavy chain gene expression during skeletal muscle hypertrophy. Biochem J 1989;257:691-698.
3. Jankala H, Harjola V, Petersen N, Harkonen M. Myosin heavy chain mRNA transform to faster isoforms in immobilized skeletal muscle: a quantitative PCR study. J Appl Physiol 1997;83:977-982.
4. Booth F, Kirby C. Changes in skeletal muscle gene expression consequent to altered weight bearing. Am J Physiol 1992;262:R329-R332.
5. Wright C, Haddad F, Qin A, Baldwin K. Analysis of myosin heavy chain mRNA expression by RT-PCR. J Appl Physiol 1997;83:1389-1396.
6. Adams G, Hather B, Baldwin K, Dudley G. Skeletal muscle myosin heavy chain composition and resistance training. J Appl Physiol 1993;74:911-915.
7. Jurimae J, Abernethy P, Blake K, McEniery M. Changes in the myosin heavy chain isoform profile of the triceps brachii muscle following 12 weeks of resistance training. Eur J Appl Physiol 1996;74:287-292.
8. Staron R, Karapondo D, Kraemer W, Fry A, Gordon S, Falkel J, Hagerman F, Hikida R. Skeletal muscle adaptations during early phase of heavy-resistance training in men and women. J Appl Physiol 1994;76:1247-1255.
9. Chesley A, MacDougall D, Tarnopolsky M, Atkinson S, Smith K. Changes in human muscle protin synthesis after resistance exercise. J Appl Physiol 1992;73:1383-1388.
10. Yarasheski K, Zachwieja J, Bier D. Acute effects of resistance exercise on muscle protein synthesis rate in young and elderly men and women. Am J Physiol 1993;265:E210-E214.
11. Welle S, Bhatt K, Thornton C. Stimulation of myofibrillar synthesis by exercise is mediated by more efficient translation of mRNA. J Appl Physiol 1999;86:1220-1225.
12. Hortabayagi T, Dempsey L, Fraser D, Zheng D, Hamilton G, Lambert J. Changes in muscle strength, muscle fibre size and myofibrillar gene expression after immobilization and retraining in humans. J Physiol 2000;524:293-304.
13. Willoughby D, Pelsue S. Muscle strength and qualitative myosin heavy chain isoform mRNA expression in the elderly after moderate- and high-intensity weight training. J Aging Phys Act 1998;6:327-339.
14. Stedman H, Satyapriya S. Molecular genetics in basic myology: A rapidly evolving perspective. Muscle Nerve 1988;11:668-682.
15. Willoughby D. The effects of weight training programs involving periodization and partially-equated volumes on upper- and lower-body strength. J Strength Conditioning Res 1993;7:2-8.
16. Willoughby D, Simpson S. The effects of combined electromyostimulation and dynamic muscular contractions on the strength on college basketball players. J Strength Conditioning Res 1996;10:34-39.
17. Andersen P, Saltin B. Maximal perfusion of skeletal muscle in man. J Physiol 1985;366:233-249.
18. Berger R. Optimum repetitions for the development of strength. Res Quart Exer Sport 1962;33:334-338.
19. Fleck S. Kraemer W. Designing Resistance Training Programs (3rd ed.). 1999; Champaingn, IL: Human Kinetics.
20. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidium thiocynate-phenol-chloroform extraction. Anal Biochem 1987;162:156-159.
21. Willoughby D, Priest J, Jennings R. Myosin heavy chain isoform and ubiquitin protease mRNA expression after passive leg cycling in persons with spinal cord injury. Arch Phys Med Rehab In Press.
22. Ennion S, Sant'Ana Pereira J, Sargent A, Young A, Goldspink G. Characterization of human skeletal muscle fibers according to the myosin heavy chains they express. J Mus Res Cell Mot 1995;16:35-43.
23. Wang A, Doyle M, Mark D. Quantitation of mRNA by the polymerase chain reaction. Biochemistry 1989;86:9717-9721.
24. Chomczynski P. A reagent for the single-step simultaneous isolation of RNA, DNA, and proteins from cell and tissue samples. BioTechniques 1993;15:532-537.
25. Willoughby D. Barnes W. Ingalls C. Smith S. Effects of the beta2-agonist clenbuterol and exercise training on muscle protein and performance in frogs. J Strength Conditioning Res 1996;10:228-233.
26. Booth F. Tseng B. Fluck M. Carson J. Molecular and cellular adaptations of muscle in response to physical training. Acta Physiol Scand 1998;162:343-350.
27. Goldspink G. Scutt A. Martindale J. Jaenicke T. Turay L. Gerlach G. Stretch and force generation induce rapid hypertrophy and myosin isoform gene switching in adult skeletal muscle. Biochem Soc Trans 1991;19:368-373.
28. Caiozzo V. Haddad F. Baker M. Baldwin K. Influence of mechanical loading on myosin heavy-chain protein and mRNA isoform expression. J Appl Physiol 1996;80:1503-1512.
29. Staron R. Hikida R. Hagerman F. Dudley G. Murray T. Human skeletal muscle fiber type adaptability to various workloads. J Histochem Cytochem 1984;32:146-152.