Evidence that isolated chloroplasts contain an integrated lipid-synthesizing assembly that channels acetate into long-chain fatty acids

Plant Physiology

Volumn 110, Issue 4, 1996, Pages 1239-1247

  Grattan Roughan, P ^a   Ohlrogge, John B ^b  

^a THE NEW ZEALAND INSTITUTE FOR PLANT AND FOOD RESEARCH LIMITED   (New Zealand)

^b MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SPINACIA OLERACEA;

EID: 0000769566     PISSN: 00320889     EISSN: None     Source Type: Journal    
DOI: 10.1104/pp.110.4.1239     Document Type: Article

References (43)

1. Bagnara AS, Finch LR (1972) Quantitative extraction and estimation of intracellular nucleoside triphosphates of Escherichia coli. Anal Biochem 45: 24-34
2. Cahoon EB, Shanklin J, Ohlrogge JB (1992) Expression of a coriander desaturase results in petroselenic acid in transgenic tobacco. Proc Natl Acad Sci USA 89: 11184-11188
3. Caughey I, Kekwick RGO (1982) The characteristics of some components of the fatty acid synthetase system in the plastids from the mesocarp of avocado (Persea americana) fruit. Eur J Biochem 123: 553-561
4. Cernovic ZG, Plesnicar M (1984) An improved procedure for the isolation of intact chloroplasts of high photosynthetic capacity. Biochem J 223: 543-545
5. 2 evolution by isolated chloroplasts. Biochem Biophys Acta 143: 614-624
6. Crofts AR, Deamer DW, Packer L (1967) Mechanisms of light-induced structural changes in chloroplasts. II. The role of ion movements in volume changes. Biochim Biophys Acta 131: 97-118
7. Douce R, Joyard J (1982) Structure and function of the plastid envelope. Adv Bot Res 7: 1-116
8. Gontero B, Cardenas ML, Ricard J (1988) A functional five-enzyme complex of chloroplasts involved in the Calvin cycle. Eur J Biochem 173: 437-443
9. Heineke D, Riens B, Grosse H, Hoferichter P, Peter U, Flügge U-I, Heldt HW (1991) Redox transfer across the inner chloroplast envelope membrane. Plant Physiol 95: 1131-1137
10. Heintze A, Riedel A, Aydogdu S, Schultz G (1994) Formation of chloroplast isoprenoids from pyruvate and acetate by chloroplasts from young spinach plants. Evidence for a mevalonate pathway in immature chloroplasts. Plant Physiol Biochem 32: 791-797
11. Hoj PB, Mikkelsen JD (1982) Partial separation of individual enzyme activities of an ACP-dependent fatty acid synthetase from barley chloroplasts. Carlsberg Res Commun 47: 119-141
12. Jacobson BS, Jaworski JG, Stumpf PK (1974) Fat metabolism in higher plants. LXII. Stearyl-acyl carrier protein desaturase from spinach chloroplasts. Plant Physiol 54: 484-486
13. Kleinig H (1989) The role of plastids in isoprenoid biosynthesis. Annu Rev Plant Physiol 40: 39-59
14. Kreuz K, Kleinig H (1981) On the compartmentation of isopentyl diphosphate synthesis and utilization in plant cells. Planta 153: 578-581
15. Libertint LJ, Smith S (1978) Purification and properties of a thioesterase from lactating mammary gland which modifies the product specificity of fatty acid synthase. J Biol Chem 253: 1393-1401
16. Lilley RMcC, Fitzgerald MP, Rienits KG, Walker DA (1975) Criteria of intactness and the photosynthetic activity of spinach chloroplast preparations. New Phytol 75: 1-10
17. Mohan SB, Keckwick RGO (1980) Aceryl-coenzyme A carboxylase from avocado (Persea americana) plastids and spinach (Spinacea oleracea) chloroplasts. Biochem J 187: 667-676
18. Murakami S, Strotmann H (1978) Adenylate kinase bound to the envelope membranes of spinach chloroplasts. Arch Biochem Biophys 185: 30-38
19. Murphy DJ, Walker DA (1982) Acetyl-coenzyme A biosynthesis in the chloroplast. What is the physiological precursor? Planta 156: 84-88
20. Ovadi J, Sere PA (1992) Channel your energies. Trends Biochem Sci 17: 445-447
21. Post-Beittenmiller D, Roughan G, Ohlrogge JB (1992) Regulation of plant fatty acid biosynthesis. Analysis of acyl-CoA and acyl-acyl carrier protein substrate pools in spinach and pea chloroplasts. Plant Physiol 100: 923-930
22. Post-Beittenmiller MA, Schmidt KM, Ohlrogge JB (1989) Expression of holo and apo forms of spinach acyl carrier protein-1 in leaves of transgenic plants. Plant Cell 1: 889-899
23. Reeves SG, Hall DO (1973) The stoichiometry (ATP/2e-) of non-cyclic photophosphorylation in isolated spinach chloroplasts. Biochim Biophys Acta 314: 66-78
24. Roughan PG (1987) Fatty acid synthesis and utilization by isolated chloroplasts. Methods Enzymol 148: 327-337
25. 2: labelling in the 1, 2, or 3 position. Biochem J 300: 355-358
26. Roughan PG, Holland R, Slack CR (1979a) On the control of long-chain fatty acid synthesis in isolated intact spinach (Spinacia oleracea) chloroplasts. Biochem J 184: 193-202
27. Roughan PG, Holland R, Slack CR, Mudd JB (1979b) Acetate is the preferred substrate for long-chain fatty synthesis in isolated spinach chloroplasts. Biochem J 184: 565-569
28. Roughan PG, Kagawa T, Beevers H (1980) On the light-dependency of fatty acid synthesis by isolated spinach chloroplasts. Plant Sci Lett 18: 221-228
29. Roughan PG, Ohlrogge JB (1994) On the assay of aceryl-CoA synthetase activity in isolated chloroplasts and leaf extracts. Anal Biochem 216: 77-82
30. Roughan PG, Slack CR (1981) Acyl-CoA may be a neglected product in studies of fatty acid synthesis by isolated chloroplasts. FEBS Lett 135: 182-186
31. Sainis JK, Harris GC (1986) The association of ribulose-1,5-bisphosphate carboxylase-oxygenase with phosphoriboisomerase and phosphoribulokinase. Biochem Biophys Res Commun 139: 947-954
32. Saski Y, Hakamada K, Suama Y, Nagano Y, Furusawa I, Matsuno R (1993) Chloroplast-encoded protein as a subunit of acetyl-CoA carboxylase in pea plants. J Biol Chem 268: 25118-25123
33. Sere PA (1987) Complexes of sequential metabolic enzymes. Annu Rev Biochem 56: 89-124
34. Shimakata T, Stumpf PK (1982) Fatty acid synthase of Spinacia oleracea leaves. Plant Physiol 69: 1257-1262
35. Springer J, Heise K-P (1989) Comparison of acetate- and pyruvate-dependent fatty acid synthesis by spinach chloroplasts. Planta 177: 417-421
36. Stafford HA (1981) Compartmentation in natural product biosynthesis by multienzyme complexes. In EE Conn ed, The Biochemistry of Plants, Vol 7. Academic Press, New York, pp 115-137
37. Stumpf PK (1972) Fat synthesis by spinach chloroplasts. Methods Enzymol 24: 394-397
38. Voelker TA, Worrel AC, Anderson L, Bleibaum J, Fan C, Hawkins DJ, Radke SE, Davies HM (1992) Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants. Science 257: 72-74
39. von Glutz G, Walter P (1975) Compartmentation of acetyl-CoA in rat liver mitochondria. Eur J Biochem 60: 147-152
40. Weaire PJ, Kekwick RGO (1975) The fractionation of fatty acid synthetase activities of avocado mesocarp plastids. Biochem J 146: 439-425
41. West KR, Wiskich JT (1973) Evidence for two phosphorylation sites associated with the electron transport chain of chloroplasts. Biochim Biophys Acta 292: 197-205
42. Winter H, Robinson DG, Heldt HW (1994) Subcellular volumes and metabolic concentrations in spinach leaves. Planta 193: 530-535
43. Wolter FP, Schmidt R, Heinz E (1992) Chilling sensitivity of Arabidopsis thaliana with genetically engineered membrane lipids. EMBO J 11: 4685-4692