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4), concentration, and purification by chromatography on silica gel.
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The logical extrapolation to the use of neat benzene or toluene as solvent is not possible for reasons of solubility.
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4), concentration, and purification by chromatography on silica gel.
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40
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Anomeric configuration is readily assigned in each case by NOE difference spectroscopy and by inspection of the 1,2-coupling constant. It is confirmed, with the exception of 6, which gave only the β-mannoside, in each case by simple application of Hudson's isorotation rule in which β-mannosides are predicted to have less positive/more negative specific rotations than their α-anomers.
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