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The contact angles were measured immediately after placing the droplet onto the substrate, disturbing the system as little as possible. Because the substrates were fairly rough, substantial contact angle hysteresis was expected and observed. The absolute error in this measurement is therefore significantly larger than the relative error and the resulting surface energy scale should be regarded as somewhat qualitative. Time-dependent variations in adsorption kinetics prevent simply using the solution ratio of alcohol to methyl-terminated thiol to set this scale.
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NIH Image is a public domain image analysis program written by Wayne Rasband at the U.S. National Institutes of Health and available from the Internet by anonymous ftp from zippy.nimh.nih.gov or on floppy disk from NTIS, 5285 Port Royal Rd., Springfield, VA 22161, part number PB93-504868. The macro is available from the authors upon request.
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