An 8 kDa Methionine-Rich Protein from Soybean (Glycine max) Cotyledon: Identification, Purification, and N-Terminal Sequence

Journal of Agricultural and Food Chemistry

Volumn 44, Issue 9, 1996, Pages 2930-2935

  Revilleza, M Jamela ^a,b   Galvez, Alfredo F ^a   Krenz, Deanne C ^a   De Lumen, Benito O ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF THE PHILIPPINES LOS BAÑOS   (Philippines)

Author keywords

Methionine rich protein; Soybean (Glycine max)

Indexed keywords

GLYCINE MAX;

EID: 0000697514     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf960063u     Document Type: Article

References (25)

1. Altenbach, S. B.; Simpson, R. B. Manipulation of the methionine-rich protein genes in plant seeds. Trends Biotechnol. 1990, 8, 156-160.
2. Altenbach, S. B.; Pearson, K. W.; Leung, F. W.; Sun, S. M. Cloning and sequence analysis of a cDNA encoding a brazil nut protein exceptionally rich in methionine. Plant Mol. Biol. 1987, 8, 239-250.
3. De Clerq, A.; Vabdewiele, M.; Van Damme, J.; Guerche, P.; Van Montagu, M.; Vandekerchove, J.; Krebbers, E. Stable accumulation of modified 2S albumin seed storage proteins with higher methionine contents in transgenic plants. Plant Physiol. 1990, 94, 970-979.
4. de Lumen, B. O. Molecular approaches to improving the nutritional and functional properties of plant seed as food sources: developments and comments. J. Agric. Food Chem. 1990, 38, 1779-1788.
5. de Lumen, B. O.; Kho, C. J. Identification of methionine containing proteins and quantification of their methionine contents. J. Agric. Food Chem. 1987, 35, 688-691.
6. de Lumen, B. O.; Thompson, S.; Odegard, W. J. Sulfur amino acid-rich proteins in Acha (Digitaria exilis), a promising underutilized African cereal. J. Agric. Food Chem. 1993, 41, 1045-1047.
7. Gayler, K. R.; Kolivas, S.; Macfarlane, A. J.; Lilley, G. G.; Baldi, M.; Blagrove, R. J.; Johnson, E. D. Biosynthesis cDNA and amino acid sequences of a precursor of conglutin o, a sulphur-rich protein from Lupinus angustifolius. Plant Mol. Biol. 1990, 15, 879-893.
8. George, A. A.; de Lumen, B. O. A novel methionine-rich protein in soybean: Identification, amino acid composition and N-terminus sequence. J. Agric. Food Chem. 1991, 39, 224-227.
9. Gupta, Y. P. Chemistry and Biochemistry of Legumes; Arora, S. K., Ed.; Oxford and IBH Publishing: New York, 1983.
10. Hoffman, L. M.; Donaldson, D. D.; Herman, E. M. A modified storage protein is synthesized, processed and degraded in the seeds of transgenic plants. Plant Mol. Biol. 1988, 11, 717-729.
11. Kho, C. J.; de Lumen, B. O. Identification and isolation of methionine and cysteine rich protein fraction in soybean seed. Plant Foods Hum. Nutr. 1988, 38, 287-296.
12. Kirihara, J. A.; Hunsperger, J. P.; Mahoney, W. C.; Messing, J. W. Differential expression of a gene for a methioninerich storage protein in maize. Mol. Gen. Genet. 1988, 211, 477-484.
13. Kortt, A. A.; Caldwell, J. B.; Lilley, G. G.; Higgins, T. J. Amino acid and cDNA sequences of a methionine-rich 2S protein from sunflower seed (Helianthus annuus L.). Eur. J. Biochem. 1991, 195, 329-334.
14. Krebbers, E.; Herdies, L.; De Clerq, A.; Seurinck, J.; Leemans, J.; Van Damme, J.; Segura, M.; Gheysen, G.; Van Montagu, M.; Vandekerckhove, J. Determination of the processing sites of an Arabidopsis 2S albumin and characterization of the complete gene family. Plant Physiol. 1988, 87, 859-866.
15. Kühn, R. M.; Caspar, T.; Dehesh, K.; Quail, P. H. DNA binding factor GT-2 from Arabidopsis. Plant Mol. Biol. 1993, 23, 337-348.
16. Lilley, G. G.; Inglis, A. S. Amino acid sequence of conglutin delta, a sulfur-rich seed protein of Lupinus angustifolius L. FEBS Lett. 1986, 195, 235-241.
17. Nordlee, J. A.; Taylor, S. L.; Townsend, J. A.; Thomas, L. A.; Townsend, R. Investigations of the allergenicity of Brazil nut 2S seed storage protein in transgenic soybean. Presented at the OECD Workshop of New Foods, Oriel College, Oxford, U.K., 1994.
18. Nordlee, J. A.; Taylor, S. L.; Townsend, J. A.; Thomas, L. A.; Bush, R. K. Identification of a Brazil-nut allergen in transgenic soybeans. New Engl. J. Med. 1996, 334, 688-692.
19. O'Farrell, P. H. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 1975, 250, 4007-4021.
20. Ptashne, M. How eukaryotic transcriptional activators work. Nature 1988, 335, 683-689.
21. Rackis, J. Amino acids in soybean hulls and oil meal fractions. J. Agric. Food Chem. 1961, 9, 409-412.
22. Schagger, H.; Von Jagow, G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 1987, 166, 368-379.
23. Shewry, P. R.; Napier, J. A.; Tatham, A. S. Seed storage proteins: structure and biosynthesis. Plant Cell. 1995, 7, 945-956.
24. Townsend, J. A.; Thomas, L. A.; Kulisek, E. S.; Daywalt, M. J.; Winter, K. R. K.; Altenbach, S. B. Improving the quality of seed proteins in soybean. In Proceedings of the 4th Biennial Conference of Molecular and Cellular Biology of Soybean, July 27-29, 1992; Iowa State University: Ames, IA, 1992; p 4.
25. Yamagata, H.; Tamura, K.; Tanaka, K.; Kasai, Z. Cell-free synthesis of rice prolamin. Plant Cell Physiol. 1986, 27, 1419-1422.