Urease Encapsulation in Nanoorganized Microshells

Nano Letters

Volumn 1, Issue 3, 2001, Pages 125-128

  Lvov, Yuri ^a   Antipov, Alexei A ^b   Mamedov, Arif ^c   Möhwald, Helmuth ^b   Sukhorukov, Gleb B ^b  

^a LOUISIANA TECH UNIVERSITY   (United States)

^b MAX PLANCK INSTITUTE OF COLLOIDS AND INTERFACES   (Germany)

^c OKLAHOMA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0000685289     PISSN: 15306984     EISSN: None     Source Type: Journal    
DOI: 10.1021/nl0100015     Document Type: Article

References (29)

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22. 4 shells. Afterwards, we decreased the solution pH to pH = 1, latex-cores were dissolved, and empty shells were washed with water to remove MF-residues. Thus, the hollow capsules were produced.
23. Confocal laser scanning microscopy. Confocal micrographs were taken with Leica TCS SP, equipped with a 100× oil immersion objective. The investigated capsule suspension was placed between glass slide and cover slip glued at the edges. Excitation wavelength 488 nm was chosen accordingly to fluorescein labels.
24. To encapsulate urease, one part of empty capsule suspension was mixed with 2 parts of 96% ethanol, and 1 part of 10 mg/mL urease solution (U-4002, Sigma) was added. After a 10 min incubation period, the capsules were centrifuged and washed with water to remove ethanol.
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