Purification and biochemical characterization of an extracellular endopolygalacturonase from Penicillium frequentons

Journal of Agricultural and Food Chemistry

Volumn 44, Issue 6, 1996, Pages 1616-1620

  Borin, Maria De Fátima ^a   Said, Suraia ^a   Fonseca, Maria José V ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Endopolygalacturonase; Penicillium frequentans

Indexed keywords

PENICILLIUM; PENICILLIUM GLABRUM;

EID: 0000683152     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf9506491     Document Type: Article

References (28)

1. Barnby, F. M.; Morpeth, F. F.; Pyle, D. L. Endopolygalacturonase production from Kluyveromyces marxianus. I. Resolution, purification, and partial characterisation of the enzyme. Enzyme Microb. Technol. 1990, 12, 891-897.
2. Behere, A.; Satyanarayan, V.; Padwal-Desai, S. R. Separation and limited characterization of three polygalacturonases of Aspergillus niger. Enzyme Microb. Technol. 1993, 15, 158-161.
3. Bensadoun, A.; Weinstein, D. Assay of proteins in the presence of interfering materials 1. Anal. Biochem. 1976, 70, 241-250.
4. Cleveland, T. E.; McCormick, S. P. Identification of pectinases produced in Cotton Bolls Infected with Aspergillus flavus. Phytopathology 1987, 77 (11), 1498-1503.
5. Coughlan, M. P. The properties of fungal and bacterial cellulases with comment on their production and application. Biotechnol. Genet. Eng. Rev. 1985, 3, 39-109.
6. Cruickshank, R. H.; Wade, G. C. Detection of pectic enzymes in pectin acrylamide gels. Anal. Biochem. 1980, 107, 177-181.
7. Davis, B. J. Disc electrophoresis. II. Method and application to human serum proteins. Ann. N. Y. Acad. Sci. 1964, 121, 404-427.
8. Dubois, M.; Gilles, K. A.; Hamilton, J. K.; Rebers, P. A.; Smith, F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 1956, 28 (3), 350-356.
9. Fonseca, M. J. V.; Said, S. Sequential production of pectinases by Penicillium frequentans. World J. Microbiol. Biotechnol, 1995, 11, 174-177.
10. Gillespie, A. M.; Cook, K.; Coughlan, M. P. Characterization of an endopolygalacturonase produced by solid-state cultures of the aerobic fungus Penicillium capsulatum. J. Biotechnol. 1990, 13, 279-292.
11. Hahn, M. G.; Bucheli, P.; Cervone, F.; Doares, S. H.; O'Neill, R. A.; Darvill, A.; Albersheim, P. Roles of cell wall constituents in plant-pathogen interactions. In Plant-Microbe Interactions; Kosuge, T., Nester, E. W., Eds.; McGraw-Hill Book: New York, 1989; pp 131-181.
12. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
13. Marcus, L.; Schejter, A. Single step chromatographic purification and characterization of the endopolygalacturonases and pectinesterases of the fungus, Botrytis cinerea Pers. Physiol. Plant Pathol. 1983, 22, 1-13.
14. Miller, G. L. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Biochem. 1959, 31, 426-428.
15. Polizeli, M. L. T. M.; Jorge, J. A.; Terenzi, H. F. Pectinase production by Neurospora crassa: purification and biochemical characterization of extracellular polygalacturonase activity. J. Gen. Microbiol. 1991, 137, 1815-1823.
16. Ried, J. L.; Collmer, A. Activity stain for rapid characterization of pectin enzymes in isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gels. Appl. Environ. Microbiol. 1985, 50 (3), 615-622.
17. Rijssel, M. V.; Gerwig, J. G. J.; Hansen, T. A. Isolation and characterization of an extracellular glycosylated protein complex from Clostridium thermosaccharolyticum with pectin methylesterase and polygalacturonate hydrolase activity. Appl. Environ. Microbiol. 1993, 59 (3), 828-836.
18. Rombouts, F. M.; Pilnik, W. Pectic enzymes. In Microbiol Enzymes and Bioconversions. Economic Microbiology; Rose, A. H., Ed.; Academic Press: New York, 1980; pp 227-282.
19. Rombouts, F. M.; Pilnik, W. Pectinases and other cell-wall degrading enzymes of industrial importance. Symbiosis 1986, 2, 79-89.
20. Said, S.; Fonseca, M. J. V.; Siéssere, V. Pectinase production by Penicillium frequentans. World J. Microbiol. Biotechnol 1991, 7, 607-608.
21. Sakellaris, G.; Nikolaropoulos, S.; Evangelopoulos, A. E. Purification and characterization of an extracellular polygalacturonase from Lactobacillus plantarum strain BA 11. J. Appl. Bacteriol. 1989, 67, 77-85.
22. Shanley, N. A.; van den Broek, L. A. M.; Voragen, A. G. J.; Coughlan, M. P. Physicochemical and catalytic properties of three endopolygalacturonases from Penicillium pinophilum. J. Biotechnol. 1993a, 28, 199-218.
23. Shanley, N. A.; van den Broek, L. A. M.; Voragen, A. G. J.; Coughlan, M. P. Isolation and characterization of an endopolygalacturonase from Phanerochaete chrysosporium. J. Biotechnol. 1993b, 28, 179-197.
24. Siéssere, V.; Said, S. Pectic enzymes production in solid-state fermentation using citrus pulp pellets by Talaromyces flavus, Tubercularia vulgaris and Penicillium charlessi. Biotechnol. Lett. 1989, 11 (5), 343-344.
25. Siéssere, V.; Fonseca, M. J. V.; Said, S. Extracellular polygalacturonases from Penicillium frequentons: separation and regulatory aspects. J. Gen. Microbiol. 1992, 138, 1801-1805.
26. Weber, K; Osborn, M. T. The reliability of molecular weight determination by dodecylsulphate-polyacrylamide gel electrophoresis. J. Biol. Chem. 1969, 244, 4406-4412.
27. Whitaker, J. R. Microbial pectinolytic enzymes. In Microbial Enzymes and Biotechnology, 2nd ed.; Fogarty, W. M., Kelly, C. T., Eds.; Elsevier Applied Science Publishers: London, 1990; pp 133-176.
28. White, C. A.; Kennedy, J. F. The carbohydrate-directed enzymes. In Carbohydrate Chemistry; Kennedy, J. F., Ed.; Clarendon Press: Oxford, U.K., 1988; pp 343-377.