Serine kinase activity of a Bacillus subtilis switch protein is required to transduce environmental stress signals but not to activate its target PP2C phosphatase

Molecular Microbiology

Volumn 30, Issue 1, 1998, Pages 189-196

  Kang, Choong Min ^a   Vijay, Kamni ^a   Price, Chester W ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; BACTERIAL PROTEIN; MUTANT PROTEIN; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN SERINE KINASE; SIGMA FACTOR;

ARTICLE; BACILLUS SUBTILIS; CONTROLLED STUDY; ENVIRONMENTAL FACTOR; ENZYME ACTIVATION; ENZYME ACTIVITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; STRESS;

AMINO ACID SEQUENCE; BACILLUS SUBTILIS; BACTERIAL PROTEINS; ENZYME ACTIVATION; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; HEAT-SHOCK PROTEINS; MOLECULAR SEQUENCE DATA; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN-SERINE-THREONINE KINASES; SACCHAROMYCES CEREVISIAE PROTEINS; SIGMA FACTOR; SIGNAL TRANSDUCTION; TRANSCRIPTION, GENETIC;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 0000681277     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1998.01052.x     Document Type: Article

References (39)

1. Adler, E., Donella-Deana, A., Arigoni, F., Pinna, L.A., and Stragier, P. (1997) Structural relationship between a bacterial developmental protein and eukaryotic PP2C protein phosphatases. Mol Microbiol 23: 57-62.
2. Akbar, S., Kang, C.M., Gaidenko, T.A., and Price, C.W. (1997) Modulator protein RsbR regulates environmental signalling in the general stress pathway of Bacillus subtilis. Mol Microbiol 24: 567-578.
3. Alper, S., Duncan, L., and Losick, R. (1994) An adenosine nucleotide switch controlling the activity of a cell type-specific transcription factor in B. subtilis. Cell 77: 195-205.
4. Alper, S., Dufour, A., Garsin, D.A., Duncan, L., and Losick, R. (1996) Role of adenosine nucleotides in the regulation of a stress response transcription factor in Bacillus subtilis. J Mol Biol 260: 165-177.
5. F during sporulation in Bacillus subtilis. Proc Natl Acad Sci USA 93: 3238-3242.
6. Barford, D., Jia, Z., and Tonks, N.K. (1995) Protein tyrosine phosphatases take off. Nature Struct Biol 2: 1043-1053.
7. B levels and activity in Bacillus subtilis. J Bacteriol 175: 2347-2356.
8. B is regulated by a binding protein (RsbW) that blocks its association with core RNA polymerase. Proc Natl Acad Sci USA 90: 2330-2334.
9. Bork, P., Brown, N.P., Hegyi, H., and Schultz, J. (1996) The protein phosphatase 2C (PP2C) superfamily: detection of bacterial homologues. Prot Sci 5: 1421-1425.
10. B by a regulatory pathway responsive to stationary-phase signals. J Bacteriol 174: 3695-3706.
11. B transcription factor of Bacillus subtilis. J Bacteriol 175: 7931-7937.
12. Cohen, P. (1989) The structure and regulation of protein phosphatases. Annu Rev Biochem 58: 453-508.
13. Das, A.K., Helps, N.R., Cohen, P.T.W., and Barford, D. (1996) Crystal structure of the protein serine/threonine phosphatase 2C at 2.0 Å resolution. EMBO J 15: 6798-6809.
14. Dufour, A., and Haldenwang, W.G. (1994) Interactions between a Bacillus subtilis anti-sigma factor (RsbW) and its antagonist (RsbV). J Bacteriol 176: 1813-1820.
15. Duncan, L., Alper, S., Arigoni, F., Losick, R., and Stragier, P. (1995) Activation of cell-specific transcription by a serine phosphatase at the site of asymmetric division. Science 270: 641-644.
16. Feucht, A., Magnin, T., Yudkin, M.D., and Errington, J. (1996) Bifunctional protein required for asymmetric cell division and cell-specific transcription in Bacillus subtilis. Genes Dev 10: 794-803.
17. Fields, S., and Song, O. (1989) A novel genetic system to detect protein - protein interactions. Nature 340: 245-246.
18. Gaits, F., Shiozaki, K., and Russell, P. (1997) Protein phosphatase 2C acts independently of stress-activated kinase cascade to regulate the stress response in fission yeast. J Biol Chem 272: 17873-17879.
19. Ho, S.N., Hunt, H.D., Horton, R.M., Pollen, J.K., and Pease, L.R. (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77: 51-59.
20. Hunter, T. (1995) Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signalling. Cell 80: 225-236.
21. Igo, M., Lampe, M., Ray, C., Schafer, W., Moran, C.P., and Losick, R. (1987) Genetic studies of a secondary RNA polymerase sigma factor in Bacillus subtilis. J Bacteriol 169: 3464-3469.
22. Kamberov, E.S., Atkinson, M.R., Chandran, P., and Ninfa, A.J. (1994) Effect of mutations in Escherichia coli glnL (ntrB), encoding nitrogen regulator II (NRII or NtrB), on the phosphatase activity involved in bacterial nitrogen regulation. J Biol Chem 269: 28294-28299.
23. B in response to environmental stress. J Bacteriol 178: 3846-3853.
24. Lawson, J.E., Park, S.H., Mattison, A.R., Yan, J., and Reed, L.J. (1997) Cloning, expression, and properties of the regulatory subunit of bovine pyruvate dehydrogenase phosphatase. J Biol Chem 272: 31625-31629.
25. Leung, J., Bouvier-Durand, M., Morris, P.-C., Guerrier, D., Chefdor, F., and Giraudat, J. (1994) Arabidopsis ABA response gene ABI1: features of a calcium-modulated protein phosphatase. Science 264: 1448-1452.
26. Maeda, T., Wurgler-Murphy, S.M., and Saito, H. (1994) A two-component system that regulates an osmosensing MAP kinase cascade in yeast. Nature 369: 242-245.
27. 2+ dependent enzyme. Methods Enzymol 159: 416-426.
28. Meyer, K., Leube, M.P., and Grill, E. (1994) A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana. Science 264: 1452-1455.
29. F, the first compartment-specific transcription factor of B. subtilis, is regulated by an anti-σ factor that is also a protein kinase. Cell 74: 735-742.
30. 37-RNA polymerase and a Bacillus subtilis chromosomal promoter. J Mol Biol 162: 709-713.
31. Perego, M. (1993) Integrational vectors for genetic manipulation in Bacillus subtilis. In Bacillus subtilis and Other Gram Positive Bacteria. Sonenshein, A.L., Hoch, J.L. and Losick, R. (eds). Washington, DC: American Society for Microbiology Press, pp. 615-624.
32. Shenolikar, S. (1994) Protein serine/threonine phosphatases - new avenues for cell regulation. Annu Rev Cell Biol 10: 56-86.
33. Shiozaki, K., and Russell, P. (1995) Counteractive roles of protein phosphatase 2C (PP2C) and a MAP kinase kinase homolog in the osmoregulation of fission yeast. EMBO J 14: 492-502.
34. +, is important in the heat shock response of Schizosaccharomyces pombe. Mol Cell Biol 14: 3742-3751.
35. Stahl, M.L., and Ferrari, E. (1984) Replacement of the Bacillus subtilis subtilisin structural gene with an in vitro-derived deletion mutation. J Bacteriol 158: 411-418.
36. Stragier, P., Bonamy, C., and Karmazyn-Campelli, C. (1988) Processing of a sporulation sigma factor in Bacillus subtilis: how morphological structure could control gene expression. Cell 52: 697-704.
37. B of Bacillus subtilis in response to environmental and metabolic stresses. J Bacteriol 177: 3771-3780.
38. B negative regulator RsbX. J Bacteriol 179: 1980-1984.
39. Yang, X., Kang, C.M., Brody, M.S., and Price, C.W. (1996) Opposing pairs of serine protein kinases and phosphatases transmit signals of environmental stress to activate a bacterial transcription factor. Genes Dev 10: 2265-2275.