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44mer was designed so that IDA moieties of two Is face each other when Is bind to purine tracts on 44mer. The length of the linker that connects two 1s in IDA dimer (Figure 3) was estimated to be 4 base pairs long according to CPK model building. Thus, 4 null base pairs were introduced in the center of 44mer.
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15
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0040377180
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note
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6 However, the melting curve for complex of 1 with 44mer was not affected by the presence of these metal ions under our experimental conditions.
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