Establishment of an experimental system for artificial germination and in vitro pollination with de-exined pollen in Nicotiana tabacum

Acta Botanica Sinica

Volumn 39, Issue 5, 1997, Pages 405-410

  Wang, Jin ^a   Xia, Hui Jun ^a   Zhou, Chang ^a   Yang, Hong Yuan ^a  

^a WUHAN UNIVERSITY   (China)

Author keywords

Artificial pollen germination; De exined pollen; In vitro pollination; Nicotiana tabacum

Indexed keywords

EID: 0000642681     PISSN: 05777496     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (13)

1. Deaprez B, Chupeau Y, Bourgin J F. Preparation and fusion properties of protoplasts from mature pollen of Nicotiana tabacum. Plant Cell Rep, 1995. 14 : 199-203
2. Li X H. Plantlet regeneration from mesophyll protoplasts of Digitalis lanata Ehrh. Theor Appl Genet, 1981. 60 : 345-347
3. Martin F W. Staining and observing pollen tubes in the style by means of fluorescence. Stain Technol, 1959. 34 : 125-128
4. Read S M, Clarke A E, Bacic A. Requirements for division of the generative nucleus in cultured pollen tubes of Nicotiana. Protoplasma, 1993. 174 : 101-115
5. Heslop-Harrison J, Knox R B, Heslop-Harrison Y. Pollen-wall proteins: Exine-held fraction associated with the incompatibility responses. Biol J Linn Soc, 1974. 7 (Suppl 1) : 189-202
6. Dumas C, Russell S D. Plant reproductive biology : Trends. In: Russell S D, Dumas C eds., Sexual Reproduction in Flowering Plants. San Diego: Academic Press, 1992. 565-592
7. Zhang H Q, Croes A F. A new medium for pollen germination in vitro. Acta Bot Neerl, 1982. 31 : 113-119
8. Jahnen W, Lush W M, Clarke A E. Inhibition of in vitro pollen tube growth by isolated S-glycoproteins of Nicotiana alata. Plant Cell, 1989. 1 : 501-510
9. Benito-Moreno R M, Macke F, Alwen A et al. In situ seed production after in vitro matured isolated pollen. Planta, 1988. 1 : 501-510
10. Tupý J, Řihová L, Zórský V. Production of fertile tobacco pollen from microspores in suspension culture and its storage for in situ pollination. Sex Plant Reprod, 1991. 4 : 281-287