Cell damage by UVA radiation of a mercury microscopy lamp probed by autofluorescence modifications, cloning assay, and comet assay

Journal of Biomedical Optics

Volumn 1, Issue 2, 1996, Pages 217-222

  König, Karsten K ^a   Krasieva, Tatjana ^b   Bauer, E ^c   Fiedler, Ursula ^c   Berns, Michael W ^b   Tromberg, Bruce J ^b   Greulich, Karl O ^c  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c FRITZ LIPMANN INSTITUTE   (Germany)

Author keywords

Autofluorescence; Cloning assay; Comet assay; Electrophoresis; NADH; Oxidative stress; UV microscopy; UVA; Vital cell microscopy

Indexed keywords

CRICETINAE; CRICETULUS GRISEUS;

EID: 0000598589     PISSN: 10833668     EISSN: None     Source Type: Journal    
DOI: 10.1117/12.233373     Document Type: Article

References (22)

1. K. König, "Johann Wilhelm Ritter - the discoverer of ultraviolet radiation," Dermatol. Mon.schr. 174, 493-497 (1988).
2. K. König and H. Schneckenburger, "Laser-induced autofluorescence for medical diagnosis," J. Fluorescence 4(1), 17-10 (1994).
3. T. G. Burchuladze, E. G. Sideris, and G. I. Fraikin, "Sensitized NADH formation of single-stranded breaks in plasmid DNA upon the action of near UV radiation," Biofizika. 35, 722-725 (1990).
4. M. L. Cunningham, J. S. Johnson, S. M. Giovanazzi, and M. J. Peak, "Photosensitized production of Superoxide anion by monochromatic (290-405 nm) ultraviolet irradiation of NADH and NADPH coenzymes," Photochem. Photobiol. 42(2), 125-128 (1985).
5. M. Peak, J. Peak, and B. Cames, "Induction of direct and indirect single strand breaks in human cell DNA by far- and near-ultraviolet radiations: Action spectrum and mechanisms," Photochem. Photobiol. 45, 381-387 (1987).
6. R. M. Tyrrell, "The interaction of UVA radiation with cultured cells," J. Photochem. Photobiol. 4, 349-361 (1990).
7. B. Chance and B. Thorell, "Localization and kinetics of reduced pyridine nucleotides in living cells by microfluorimetry," J. Biol. Chem. 234, 3044-3050 (1959).
8. C. Y. Guezennec, F. Lienhard, F. Louisy, G. Renault, M. H. Tusseau, and P. Portero, "In situ NADH laser fluorimetry during muscle contraction in humans," Eur. J. Appl. Physiol. 63, 36-42 (1991).
9. H. Schneckenburger, P. Gessler, and I. Pavenstädt-Grupp, "Measurement of mitochondrial deficiencies in living cells by microspectrofluorometry," J. Histochem. Cytochem. 40(10), 1573-1578 (1992).
10. H. Schneckenburger and K. König, "Fluorescence decay kinetics and imaging of NAD(P)H and flavins as metabolic indicators," Opt Eng. 31(7), 1447-1451 (1992).
11. O. Östling and K. J. Johanson, "Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells," Biochem. Biophys. Res. Comm. 123(1), 291-298 (1984).
12. N. P. Singh, M. T. McCoy, E. E. Tice, E. L. Schneider, "A simple technique for quantitation of low levels of DNA damage in individual cells," Exp. Cell Res. 175, 184-191 (1988).
13. P. L. Olive, J. P. Banath, R. E. Durand, "Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the "Comet" assay," Radiat. Res. 122, 86-94 (1990).
14. R. R. Tice, P. W. Andrews, and N. P. Singh, "The single cell gel assay: A sensitive technique for evaluating intracellular differences in DNA damage and repair," in DNA Damage and Repair in Human Tissues, B. M. Sutherland and A. D. Woodhead, Eds., Plenum Press, New York (1990).
15. C. M. Gedik, S. W. B. Ewen, and A. R. Collins, "Single-cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells," Int. J. Radiat. Biol. 62(3), 313-320 (1992).
16. C. F. Arlett, J. E. Lowe, S. A. Harcourt, A. P. W. Waugh, J. Cole, L. Roza, B. L. Diffey, T. Mori, O. Nikaido, and M. H. L. Green, "Hypersensitivity of human lymphocytes to UV-B and solar irradiation," Cancer Res 53, 609-614 (1993).
17. A. de With, G. Leitz, and K. O. Greulich, "UV-B laser induced DNA damages in lymphocytes observed by single-cell gel electrophoresis," J. Photochem. Photobiol. B 24, 47-53 (1994).
18. A. S. Dvornikov, J. Malkin, and P. M. Retzepis, "Spectroscopy and kinetics of photochromic materials for 3D optical memory devices," J. Phys. Chem. 98, 6746-6752 (1994).
19. R. Lubart, Y. Wollman, H. Friedmann, S. Rochkind, and I. Laulicht, "Effects of visible and near-infrared lasers on cell cultures," J. Photochem. Photobiol. B 12, 305-310 (1992).
20. W. Denk, J. H. Strickler, and W. W. Webb, "Two-photon laser scanning fluorescence microscopy," Science 248, 73-76 (1990).
21. K. König, P. So, W. W. Mantulin, E. Gratton, M. W. Berns, and B. J. Tromberg, "Two-photon excited cellular autofluorescence induced by cw and femtosecond NIR microirradiation," Proc. SPIE 2628, 12-19 (1995).
22. K. König, H. Liang, M. W. Berns, and B. J. Tromberg, "Cell damage by near-IR microbeams," Nature 377, 20-21 (1995).