A method for rapid freeze fixation of plant cells

Protoplasma

Volumn 131, Issue 2, 1986, Pages 153-165

  Lancelle, Susan A ^a   Callaham, D A ^a   Hepler, P K ^a  

^a UNIVERSITY OF MASSACHUSETTS   (United States)

Author keywords

Freeze substitution; Hafnium tetrachloride; Plant cell ultrastructure; Rapid freeze fixation

Indexed keywords

EID: 0000583329     PISSN: 0033183X     EISSN: 16156102     Source Type: Journal    
DOI: 10.1007/BF01285037     Document Type: Article

References (30)

1. The structure of cytoplasm in directly frozen cultured cells. I. Filamentous meshworks and the cytoplasmic ground substance
2. An ultrastructural and cytochemical study of the wall-membrane apparatus using freeze substitution
3. Ultra-rapid freezing of thin biological samples
4. The direct measurement of temperature changes within freeze fracture specimens during rapid quenching in liquid coolants
5. Bacterial mesosomes: method dependent artifacts
6. A freeze substitution and freeze fracture study of bacterial spore structures
7. New instruments which facilitate freezing at 83K and 6K
8. Structure of functional soybean sieve elements
9. The design and use of a simple device for rapid quench-freezing of biological samples
10. Visualization of the cytoskeletal elements in tissue culture cells by bloc-staining with hafnium chloride after rapid freezing and freeze substitution fixation
11. Mitosis in the fungus Zygorhynchus moelleri: evidence for stage specific enhancement of microtubule preservation by freeze substitution
12. The blepharoplast of Marsilea: its de novo formation and spindle association
13. Synaptic vesicle exocytosis captured by quick freezing and correlated with quantal transmitter release
14. Filament organization revealed in platinum replicas of freeze-dried cytoskeletons
15. Ultrastructure of freeze-substituted hyphae of the basidiomycete Laetisaria arvalis
16. Ultrastructural analysis of hyphal tip cell growth in fungi: spitzenkorper, cytoskeleton and endomembranes after freeze substitution
17. Hyphal tip ultrastructure of the fungus Fusarium
18. Ultrastructure of freeze-substituted Frankia strain HFPCcI3, the actinomycete isolated from root nodules of Casuarina cunninghamiana
19. The stabilization of labile configurations of plant cytoplasm by freeze substitution
20. Microtubules around migrating nuclei in conventionally fixed and freeze-substituted cells
21. Monitoring the course of fixation of plant cells
22. Beginning of exocytosis captured by rapid freezing of Limulus amebocytes
23. Cryofixation: a tool in biological ultrastructural research
24. The structure of the cytoplasmic matrix preserved by freeze drying and freeze substitution
25. A new look at some cooling rates using a purpose designed rate meter
26. Ultrastructural studies on tapetum and pollen development
27. Cytoskeleton and integration of cellular function in cells of higher plants
28. Actomyosin structure in contracting muscle detected by rapid freezing
29. Myosin filaments in smooth muscle cells of the guinea pig taenia coli: a freeze substitution study
30. Rapid freezing and electron microscopy for the arrest of physiological processes