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+; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; TTN, total turnover number.
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In some cases, the cell-free extracts can catalyze highly stereoselective reductions. For example, see: (c) Keinan, E.; Seth, K. K.; Lamed, R. J. Am. Chem. Soc. 1986, 108, 3474.
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25
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85034476500
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note
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6a
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31
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35
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85034459715
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note
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Control experiments were done without NADP(H), G6PDH, and G6P in 10 mM phosphate buffer (pH 7.0). Conversions of 5 to 15 in the presence and absence of the reductase (1.5 U with respect to 1) after 4 h were 82% and 54%, respectively.
-
-
-
-
36
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85034478825
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note
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The weight of the reductase used in the preparative reduction was ca. 0.32 mg as determined by the method of Bradford. Because the molecular weight of the reductase is 37 000, the amount of reductase used is 8.6 nmol.
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38
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0029969716
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