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Volumn 63, Issue 15, 1998, Pages 4996-5000

Highly Enantioselective Reduction of Carbonyl Compounds Using a Reductase Purified from Bakers' Yeast

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EID: 0000430160     PISSN: 00223263     EISSN: None     Source Type: Journal    
DOI: 10.1021/jo980165e     Document Type: Article
Times cited : (44)

References (47)
  • 8
    • 85034461028 scopus 로고    scopus 로고
    • note
    • +; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; TTN, total turnover number.
  • 14
    • 33845375365 scopus 로고
    • In some cases, the cell-free extracts can catalyze highly stereoselective reductions. For example, see: (c) Keinan, E.; Seth, K. K.; Lamed, R. J. Am. Chem. Soc. 1986, 108, 3474.
    • (1986) J. Am. Chem. Soc. , vol.108 , pp. 3474
    • Keinan, E.1    Seth, K.K.2    Lamed, R.3
  • 25
    • 85034476500 scopus 로고    scopus 로고
    • note
    • 6a
  • 35
    • 85034459715 scopus 로고    scopus 로고
    • note
    • Control experiments were done without NADP(H), G6PDH, and G6P in 10 mM phosphate buffer (pH 7.0). Conversions of 5 to 15 in the presence and absence of the reductase (1.5 U with respect to 1) after 4 h were 82% and 54%, respectively.
  • 36
    • 85034478825 scopus 로고    scopus 로고
    • note
    • The weight of the reductase used in the preparative reduction was ca. 0.32 mg as determined by the method of Bradford. Because the molecular weight of the reductase is 37 000, the amount of reductase used is 8.6 nmol.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.