Phytohormone-induced GABA production in transformed root cultures of Datura stramonium: An in vivo 15N NMR study

Journal of Experimental Botany

Volumn 47, Issue 299, 1996, Pages 811-818

  Ford, Y Y ^a,c   Ratcliffe, R G ^a   Robins, R J ^b,d  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b INSTITUTE OF FOOD RESEARCH   (United Kingdom)

^c UNIVERSITY OF WARWICK   (United Kingdom)

^d UNIVERSITÉ DE NANTES   (France)

Author keywords

Datura; Nitrogen metabolism; NMR; Root cultures; aminobutyric acid

Indexed keywords

DATURA; DATURA STRAMONIUM;

EID: 0000401339     PISSN: 00220957     EISSN: None     Source Type: Journal    
DOI: 10.1093/jxb/47.6.811     Document Type: Article

References (34)

1. Aird ELH, Hamill JD, Robins RJ, Rhodes MJC. 1988a. Chromosome stability in transformed hairy root cultures and the properties of variant lines of Nicotiana rustica hairy roots. In: Robins RJ, Rhodes MJC, eds. Manipulating secondary metabolism in culture. Cambridge University Press, 137-44.
2. Aird ELH, Hamill JD, Rhodes MJC. 1988b. Cytogenetic analysis of hairy root cultures from a number of species transformed by Agrobacterium rhizogenes. Plant Cell, Tissue and Organ Culture 15, 47-57.
3. 15N-NMR. Journal of Experimental Botany 43, 633-9.
4. Carroll AD, Fox GG, Laurie S, Phillips R, Ratclifle RG, Stewart GR. 1994. Ammonium assimilation and the role of γ-aminobutyric acid in pH homeostasis in carrot cell suspensions. Plant Physiology 106, 513-20.
5. Carroll AD, Stewart GR, Phillips R. 1992. Dynamics of nitrogenous assimilate partitioning between cytoplasmic and vacuolar fractions in carrot cell suspension cultures. Plant Physiology 100, 1808-14.
6. Crawford LA, Bown AW, Breitkreuz KE, Guinel FC. 1994. The synthesis of γ-aminobutyric acid in response to treatments reducing cytosolic pH. Plant Physiology 104, 865-71.
7. Flores HE. 1987. Use of plant cells and organ culture in the production of biological chemicals. In: LeBaron HM, Mumma RO, Honeycutt RC, Duesing JH, eds. Biotechnology in agricultural chemistry. Washington: American Chemical Society, 66-86.
8. Ford Y-Y. 1995. Metabolic studies of transformed roots. D Phil, thesis, University of Oxford.
9. 15N NMR studies of secondary metabolism in transformed root cultures of Datura stramonium and Nicotiana tabacum. Phytochemistry 36, 333-9.
10. Gamborg OL, Miller RA, Ojima K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research 50, 151-8.
11. Joy KW. 1988. Ammonia, glutamine and asparagine: a carbon-nitrogen interface. Canadian Journal of Botany 66, 2103-9.
12. Lea PJ, Blackwell RD, Joy KW. 1992. Ammonia assimilation in higher plants. In: Mengel K, Pilbeam DJ, eds. Nitrogen metabolism of plants. Oxford: Clarendon Press, 153-86.
13. 15N ammonium assimilation. Journal of Experimental Botany 45, 767-77.
14. 14C study of the role of the leaf in the nitrogen nutrition of the seed of Datura stramonium L. Journal of Experimental Botany 26, 361-6.
15. 15N incorporation and glutamine synthetase inhibition studies of nitrogen assimilation in leaves of the nitrophile, Datura stramonium L. New Phylologist 81, 519-26.
16. Ling V, Snedden WA, Shelp BJ, Assmann SM. 1994. Analysis of a soluble calmodulin binding protein from fava bean roots: identification of glutamate decarboxylase as a calmodulin-activated enzyme. The Plant Cell 6, 1135-43.
17. Lipp João KH, Brown TA. 1994. Long-term stability of root cultures of tomato transformed with Agrobacterium rhizogenes R1601. Journal of Experimental Botany 45, 641-7.
18. Nyman S. 1994. Incorporation of arginine, ornithine and phenylalanine into tropane alkaloids in suspension-cultured cells and aseptic roots of intact plants of Atropa belladonna. Journal of Experimental Botany 45, 979-86.
19. Nyman S, Söderman A, Simola LK. 1993. Metabolism of arginine and ornithine in suspension-cultured cells and aseptic roots of intact plants of Atropa belladonna. Journal of Experimental Botany 44, 869-77.
20. Probyn TA, Lewis OAM. 1979. The route of nitrate-nitrogen assimilation in the root of Datura stramonium L. Journal of Experimental Botany 30, 299-305.
21. Raven JA. 1985. pH regulation in plants. Science Progress 69, 495-509.
22. Rhodes MJC, Robins RJ, Aird ELH, Payne J, Parr AJ, Walton NJ. 1989. Regulation of secondary metabolism in transformed root cultures. In: Kurz WGW, ed. Primary and secondary metabolism of plant cell cultures. Berlin: Springer-Verlag, 58-72.
23. Rhodes MJC, Robins RJ, Hamill JD, Parr AJ, Hilton MG, Walton NJ. 1990. Properties of transformed root cultures. In: Charlwood BV, Rhodes MJC, eds. Secondary products from plant tissue culture, Oxford: Clarendon Press, 201-25.
24. Roberts JKM, Hooks MA, Miaullis AP, Edwards S, Webster C. 1992. Contribution of malate and amino acid metabolism to cytoplasmic pH regulation in hypoxic maize root tips studied using nuclear magnetic resonance spectroscopy. Plant Physiology 98, 480-7.
25. Robins RJ, Bent EG, Rhodes MJC. 1991b. Studies on the biosynthesis of tropane alkaloids by Datura stramonium L. transformed root cultures. 3. The relationship between morphological integrity and alkaloid biosynthesis. Planta 185, 385-90.
26. Robins RJ, Parr AJ, Bent EG, Rhodes MJC. 1991a. Studies on the biosynthesis of tropane alkaloids in Datura stramonium L. transformed root cultures. 1. The kinetics of alkaloid production and the influence of feeding intermediate metabolites. Planta 183, 185-95.
27. Robins RJ, Walton NJ. 1993. The biosynthesis of tropane alkaloids. In: Cordell GA, ed. The alkaloids. New York: Academic Press, 116-87.
28. Robinson SA, Slade AP, Fox GG, Phillips R, Ratcliffe RG, Stewart GR. 1991. The role of glutamate dehydrogenase in plant nitrogen metabolism. Plant Physiology 95, 509-16.
29. Snedden WA, Arazi T, Fromm H, Shelp BJ. 1995. Calcium/ calmodulin activation of soybean glutamate decarboxylase. Plant Physiology 108, 543-9.
30. Spickett CM, Smirnoff N, Ratcliffe RG. 1993. An in vivo nuclear magnetic resonance investigation of ion transport in maize (Zea mays) and Spartina anglica roots during exposure to high salt concentration. Plant Physiology 102, 629-38.
31. Tabata M, Yamamoto H, Hiraoka N, Marumoto Y, Konoshima M. 1971. Regulation of nicotine production in tobacco tissue culture by plant growth regulators. Phytochemistry 10, 723-9.
32. Tepfer D. 1990. Genetic transformation using Agrobacterium rhizogenes. Physiologia Plantarum 79, 140-6.
33. 15N nuclear magnetic resonance study of nitrogen metabolism in shoot-forming cultures of white spruce (Picea glauca) buds. Plant Physiology 91, 193-200.
34. Toivonen L. 1993. Utilization of hairy root cultures for production of secondary metabolites. Biotechnology Progress 9, 12-20.