Interaction between bovine serum albumin and saponin as studied by heat stability and protease digestion

Journal of Agricultural and Food Chemistry

Volumn 44, Issue 3, 1996, Pages 792-795

  Ikedo, Shingo ^a   Shimoyamada, Makoto ^a   Watanabe, Kenji ^a  

^a GIFU UNIVERSITY   (Japan)

Author keywords

Bovine serum albumin; ELISA; Heat stability; Immunodiffusion; Interaction; Protease resistance; Soyasaponin

Indexed keywords

BOVINAE;

EID: 0000351667     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf940742+     Document Type: Article

References (13)

1. Chen, Y.-H.; Yang, J, T.; Chau, K. H. Determination of the helix and β form of proteins in aqueous solution by circular dichroism. Biochemistry 1974, 13, 3350-3359.
2. Gumpen, S.; Hegg, P. O.; Martens, H. Thermal stability of fatty acid-serum albumin complexes studied by differential scanning calorimetry. Biochim. Biophys. Acta 1979, 574, 189-196.
3. Hirayama, K.; Akashi, S.; Furuya, M.; Fukuhara, K. Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and FRIT-FAB LCVMS. Biochem. Biophys. Res. Commun. 1990, 173, 639-646.
4. Kato, A.; Fujimoto, K.; Matsudomi, N.; Kobayashi, K. Protein flexibility and functional properties of heat-denatured ovalbmnin and lysozyme. Agric. Biol. Chem. 1986, 50, 417-420.
5. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 1970, 227, 680-685.
6. Lowry, O. H.; Rosebrough, N. J.; Farr, A. L.; Randall, R. J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951, 193, 265-275.
7. Matsudaira, P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J. Biol. Chem. 1987, 262, 10035-10038.
8. Oakenfull, D. Saponins in food - a review. Food Chem. 1981, 6, 19-40.
9. Ouchterlony, O. Antigen-antibody reactions in gels. Acta Pathol. Microbiol. 1949, 26, 507-515.
10. Potter, S. M.; Jimenez-Flores, R.; Pollack, J.; Lone, T. A.; Berber-Jimenez, M. D. Protein-saponin interaction and its influence on blood lipids. J. Agric. Food Chem. 1993, 41, 1287-1291.
11. Price, K. R.; Johnson, I. T.; Fenwick, G. R. The chemistry and biological significance of saponins in foods and feedingstuffs. CRC Crit. Rev. Food Sci. Nutr. 1987, 26, 27-135.
12. Reed, R. G.; Feldhoff, R. C.; Clute, O. L.; Peters, T., Jr. Fragments of bovine serum albumin produced by limited proteolysis. Conformation and ligand binding. Biochemistry 1975, 14, 4578-4583.
13. Sidhu, G. S.; Oakenfull, D. G. A mechanism for the hypocholerterolaemic activity of saponins. Br. J. Nutr. 1986, 55, 643-649.