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note
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Interference of vesicle light scattering on particle absorbance measurements is eliminated by collapsing vesicle compartments with the nonionic detergent octyl β-D-glucopyranoside (OG), prior to scanning.
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85033928185
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Cd.
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85033907621
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Thin aqueous specimens were prepared on 300 mesh, lacey carbon substrate, copper TEM grids (Ted Pella, Redding, CA) inside a temperature-and humidity-controlled environmental chamber at 25 °C. All vesicle solutions were diluted with MilliQ water, to a lipid concentration of approximately 1 mg/mL for specimen preparation. A thin film of liquid was produced by blotting a sample droplet with Whatman chemical-grade filter paper. The specimens were vitrified by plunging into a liquid propane-ethane mixture cooled to -180°C. Vitrified specimens were transferred under liquid nitrogen to a Gatan Model 626 Cryotransfer System (Gatan, Inc., Warrendale, PA) and were inserted into the microscope for imaging at -165°C.
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85033912654
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note
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Images are recorded on Kodak SO163 Electron Imaging Film and developed in a 1:1 Kodak D19 solution for 12 min. The micrograph negatives are scanned electronically with an AGFA Arcus II scanner at 400 dpi and receive minimal contrast adjustment using Adobe Photoshop. All features recognized in the scanned images are distinguished easily in the original micrographs.
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