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Volumn 10, Issue 8, 1998, Pages 2116-2119

Cryo-Transmission Electron Microscopy Confirms Controlled Synthesis of Cadmium Sulfide Nanocrystals within Lecithin Vesicles

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Indexed keywords


EID: 0000315757     PISSN: 08974756     EISSN: None     Source Type: Journal    
DOI: 10.1021/cm970744k     Document Type: Article
Times cited : (25)

References (33)
  • 2
    • 12044256704 scopus 로고
    • Mann, S. Nature 1993, 365, 499-505.
    • (1993) Nature , vol.365 , pp. 499-505
    • Mann, S.1
  • 10
    • 0000739296 scopus 로고
    • Reed, M. Sci. Am. 1993, 268, 118.
    • (1993) Sci. Am. , vol.268 , pp. 118
    • Reed, M.1
  • 25
    • 85033925816 scopus 로고    scopus 로고
    • note
    • Interference of vesicle light scattering on particle absorbance measurements is eliminated by collapsing vesicle compartments with the nonionic detergent octyl β-D-glucopyranoside (OG), prior to scanning.
  • 27
    • 85033928185 scopus 로고    scopus 로고
    • note
    • Cd.
  • 28
    • 85033907621 scopus 로고    scopus 로고
    • note
    • Thin aqueous specimens were prepared on 300 mesh, lacey carbon substrate, copper TEM grids (Ted Pella, Redding, CA) inside a temperature-and humidity-controlled environmental chamber at 25 °C. All vesicle solutions were diluted with MilliQ water, to a lipid concentration of approximately 1 mg/mL for specimen preparation. A thin film of liquid was produced by blotting a sample droplet with Whatman chemical-grade filter paper. The specimens were vitrified by plunging into a liquid propane-ethane mixture cooled to -180°C. Vitrified specimens were transferred under liquid nitrogen to a Gatan Model 626 Cryotransfer System (Gatan, Inc., Warrendale, PA) and were inserted into the microscope for imaging at -165°C.
  • 32
    • 85033912654 scopus 로고    scopus 로고
    • note
    • Images are recorded on Kodak SO163 Electron Imaging Film and developed in a 1:1 Kodak D19 solution for 12 min. The micrograph negatives are scanned electronically with an AGFA Arcus II scanner at 400 dpi and receive minimal contrast adjustment using Adobe Photoshop. All features recognized in the scanned images are distinguished easily in the original micrographs.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.