Substrate specificity of aqualysin I, a bacterial thermophilic alkaline serine protease from thermus aquaticus YT-1: Comparison with proteinase K, subtilisin BPN′ and subtilisin carlsberg

Bioscience, Biotechnology and Biochemistry

Volumn 62, Issue 11, 1998, Pages 2161-2165

  Tanaka, Terumichi ^a,b   Matsuzawa, Hiroshi ^a   Ohta, Takahisa ^a,c  

^a UNIVERSITY OF TOKYO   (Japan)

^b TOYOHASHI UNIVERSITY OF TECHNOLOGY   (Japan)

^c KOGAKUIN UNIVERSITY   (Japan)

Author keywords

Alkaline serine protease; Aqualysin I; Substrate specificity; Subtilisin; Thermus aquaticus YT 1

Indexed keywords

EID: 0000285358     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.62.2161     Document Type: Article

References (18)

1. Matsuzawa, H., Hamaoki, M., and Ohta, T., Production of thermophilic extracellular proteases (aqualysins I and II) by Ther-mus aquaticus YT-1, an extreme thermophile. Agric. Biol. Chem., 47, 25-28 (1983).
2. Matsuzawa, H., Tokugawa, K., Hamaoki, M., Mizoguchi, M., Taguchi, H., Terada, I., Kwon, S-T., and Ohta, T., Purification and characterization of aqualysin I (a thermophilic alkaline serine protease) produced by Thermus aquaticus YT-1. Eur. J. Biochem., 171, 441-447 (1988).
3. Kwon, S-T., Terada, I., Matsuzawa, H., and Ohta, T., Nucleotide sequence of the gene for aqualysin I (a thermophilic alkaline serine protease) of Thermus aquaticus YT-1 and characteristics of the deduced primary structure of the enzyme. Eur. J. Biochem., 173, 491-497 (1988).
4. Kwon, S-T., Matsuzawa, H., and Ohta, T., Determination of the positions of the disulfide bonds in aqualysin I (a thermophilic alkaline serine protease) of Thermus aquaticus YT-1. J. Biochem., 104, 557-559 (1988).
5. 2- and COOH-terminal pro-sequences and its processing in Escherichia coli. J. Biol. Chem., 265, 6576-6581 (1990).
6. 2-terminal pro-sequence in the position of active aqualysin I (a thermophilic serine protease) in Escherichia coli. Agric. Biol. Chem., 55, 3027-3032 (1991).
7. 2-terminal pro-region aids the production of active aqualysin I (a thermophilic protease) without the COOH-terminal pro-se-quence in Escherichia coli. FEMS Microbiol. Lett., 92, 73-78 (1992).
8. Wright, C. S., Alden, R. A., and Kraut, J., Crystal structure of a subtilisin BPN' complex with N-benzoyl-L-arginine. J. Mol. Biol., 66, 283-289 (1972).
9. Robertus, J. D., Alden, R. A., Birktoft, J. J., Kraut, J., Powers, J. C., and Wilcox, P. E., An X-ray crystallographic study of the binding peptide chloromethyl ketone inhibitors to subtilisin BPN'. Biochemistry, 11, 2439-2449 (1972).
10. Matthews, D. A., Alden, R. A., Birktoft, J. J., Freer, S. T., and Kraut, J., X-ray crystallographic study of boronic acid adducts with subtilisin BPN' (Novo). J. Biol. Chem., 250, 7120-7126 (1975).
11. Bode, W., Papamokos, E., and Musil, D., The high-resolution X-ray crystal structure of the complex formed between subtilisin Carlsberg and eglin c, an elastase inhibitor from the leech Hiru-do medicinalis. Eur. J. Biochem., 166, 673-692 (1987).
12. Betzel, C., Pal, G. P., and Saenger, W., Three-dimensional structure of proteinase K at 0.15-nm resolution. Eur. J. Biochem., 178, 155-171 (1988).
13. Morihara, K., and Tsuzuki, H., Specificity of proteinase K from Tritirachium album Limber for synthetic peptides. Agric. Biol. Chem., 39, 1489-1492 (1975).
14. Morihara, K., Oka, T., and Tsuzuki, H., Comparative study of various serine alkaline proteinases from microorganisms. Archi. Biochemi. Biophys., 165, 72-79 (1974).
15. Vidals, F. J., Bernabeu, C., and Ballesta, P. G., Peptidyl transfer ribonucleic acid hydrolase activity of proteinase K. Biochemistry, 18, 4155-4158 (1979).
16. Grøn, H., Meldal, M., and Breddam, K., Extensive comparison of the substrate preferences of two subtilisins as determined with peptide substrates which are based on the principle of intramolecular quenching. Biochemistry, 31, 6011-6018 (1992).
17. Tanaka, T., Matsuzawa, H., and Ohta, T., Stability of thermostable enzyme, aqualysin I; a subtilisin-type serine protease from Thermus aquaticus YT-1. Biosci. Biotechnol. Biochem., 62, 1806-1808 (1998).
18. Morihara, K., and Oka, T., Effect of secondary interaction on the enzymatic activity of subtilisin BPN': comparison with a-chymotrypsin, trypsin, and elastase. FEBS Lett., 33, 54-56 (1973).